Rapid and Sensitive Determination of Timosaponin AIII in Rat Plasma by LC-MS/MS and Its Pharmacokinetic Application

A rapid sensitive and selective liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed for determination of timosaponin AIII (TA-III) in rat plasma, using ginsenoside Re as an internal standard (IS). TA-III and the IS were detected in MRM mode with a negative ionization electrospray mass spectrometer. The calibration curves were linear over the concentration ranges from 11.14 to 1114 ng/mL and the lower limit of quantification (LLOQ) was 11.14 ng/mL. Intra-day and inter-day precisions (RSD) were within 10%, and accuracy ranged from 6.4% to 9.1%. The extraction recovery at three concentrations ranged from 92.3% to 95.5%. The validated method was successfully applied to monitor the concentrations of TA-III in rat plasma after intragastric administration. The best fit pharmacokinetic model to estimate the pharmacokinetic parameters was a single compartment model with weight of 1/x2 for oral administration groups of rats for TA-III

Additional file 1: Table S1. of Identification and functional characterization of the sulfate transporter gene GmSULTR1;2b in soybean

Information concerning the 28 soybean SULTR genes. Table S2. Accession numbers of putative SULTR protein sequences. Figure S1. In silico expression profiling of 28 soybean SULTR genes in 10 soybean tissues. Figure S2. Alignment of SULTR protein sequences. Figure S3. Subcellular localization of GmSULTR1;2b. Figure S4. RT-PCR assay of GmSULTR1;2b in different tissues of GmSULTR1;2b-overexpressing tobacco plants. Figure S5. Chlorophyll content of the leaves of GmSULTR1;2b-overexpressing and control tobacco plants grown under +S conditions. (DOCX 909 kb

Additional file 2: Table S3. of Identification and functional characterization of the sulfate transporter gene GmSULTR1;2b in soybean

Annotations of 227 genes with changes in expression levels greater than 2-fold. (XLSX 30 kb