DataSheet_1_The relationship between frequent premature ventricular complexes and epicardial adipose tissue volume.docx

BackgroundEpicardial adipose tissue (EAT) is related to atrial fibrillation. The association between EAT volume and premature ventricular complexes (PVCs) remains unclear. Our study aimed to investigate the effect of EAT volume on the risk of frequent PVCs and burden levels of PVCs.MethodsThis observational study retrospectively recruited consecutive patients who had consultation between 2019 and 2021 at the First Affiliated Hospital of Zhengzhou University. Frequent PVC patients (n = 402) and control patients (n = 402) undergoing non-contrast computed tomography (CT) were enrolled. We selected evaluation criteria for the conduct of a 1:1 propensity score matching (PSM) analysis. Multivariable logistic analysis was used to investigate factors related to frequent PVCs. Furthermore, the determinants of EAT volume and the burden levels of PVCs were evaluated.ResultsPatients with PVCs had a significantly larger EAT volume than control patients. EAT volume was significantly larger in male PVC patients with BMI ≥24 kg/m2, diabetes mellitus, and E/A ratio ConclusionsEAT volume was larger in frequent PVC patients than in control patients, regardless of other confounding factors. A large EAT volume was independently associated with high burden levels of PVCs. EAT volume may be a new mechanism to explain the pathogenesis of PVCs.</p

Appendix_Tables_1-4 – Supplemental material for Observation of local cardiac electrophysiological changes during off-pump coronary artery bypass grafting using epicardial mapping

<p>Supplemental material, Appendix_Tables_1-4 for Observation of local cardiac electrophysiological changes during off-pump coronary artery bypass grafting using epicardial mapping by Qin Li, Haiming Li, Liangshan Wang, Changcheng Liu, Songnan Li, Yingwei Chen, Yafei Zhang and Chengxiong Gu in Perfusion</p

Generation of p125 phage sub-libraries.

§<p><i>different residues of the 12mer p125 were randomly substituted with 20 aa to generate the successive</i> phage libaries.</p><p>*In addition, residues found to exhibit restricted substitution were specifically substituted in generation of the subsequent libraries.</p>Δ<p>8H5 reactive phage clones were identified; the peptides were sequenced.</p

8H5 reactivity and dissociation constants of p125 and related 12mer peptides.

†<p>Residues which could be randomly substituted shown in parenthesis.</p>‡<p> <i>The 8H5 reactivity of phage bearing p125 and the related 12mer peptides was determined by titration as in </i><a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0024144#pone-0024144-g001" target="_blank"><i>Figure 1</i></a><i> and expressed in titer defined as the phage dilution yielding an OD value of 1.5 (T_od1.5).</i></p><p>*<i>8H5 reactivity of the respective peptides was determined as in </i><a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0024144#pone-0024144-g002" target="_blank"><i>Figure 2</i></a><i> and expressed in blocking dose, defined as amount of the peptide that block binding of 8H5 to the indicated strain of the H5N1 avian influenza virus by 50%.</i></p>§<p> <i>Affinity of the peptides for 8H5 was determined by surface plasma resonance using BiaCore and expressed as dissociation constant (Kd).</i></p

Additional file 1 of Non-insulin-based insulin resistance indexes in predicting atrial fibrillation recurrence following ablation: a retrospective study

Additional file: Figure S1. Receiver operating curve for the use of four insulin resistance indexes in the detection of AF recurrence following ablation. Figure S2. Kaplan–Meier estimated event rates of AF recurrence following ablation according to tertiles of insulin resistance indexes. Figure S3. Kapla-Meier estimated event rates of AF recurrence following ablation according to cut-off values of ROC curves for insulin resistance indexes Figure S4. The calibration plots for the adjusted model predicting AF recurrence. Figure S5. Receiver operating curve for risk factors in the detection of AF recurrence following ablation. Figure S6. Association between TyG index (per 1 unit increase) and AF recurrence following ablation in different subgroups. Figure S7. Association between TG/HDL-C ratio (per 1 unit increase) and AF recurrence following ablation in different subgroups. Figure S8. Restricted cubic spline curves for AF recurrence by METS-IR ans TyG-BMI index after covariate adjustment in DM and non-DM patients. Figure S9. Restricted cubic spline curves for AF recurrence by METS-IR and TyG-BMI index after covariates adjustment in duration of AF ≥ 24 and < 24 months patients. Additional file: Table S1. Association between non-insulin-based IR indexes and AF recurrence after ablation stratified by the statins medication at admission. Table S2. Association between METS-IR or TyG-BMI index with DM or non-DM and AF recurrence after ablation. Table S3. Added predictive ability and reclassification statistics of METS-IR and TyG-BMI index in DM and non-DM patients. Table S4. Association between METS-IR or TyG-BMI index with duration of AF ≥ 24 or < 24months and AF recurrence after ablation. Table S5. Added predictive ability and reclassification statistics of METS-IR and TyG-BMI index in duration of AF ≥ 24 and < 24 months patients

Titration of phage displaying p125 and related 12mer peptides.

<p>10¹³ phage displaying the p125 or the related 12mer peptides, V-1b, II-1c, III-2a, IV-1b, and IV-48 was serially diluted and allowed to react with microplates previously coated with 8H5 for 30 min. The plates were in turn reacted with anti-phage. The 8H5 binding activity of the phage is determined from the titration curves and expressed as the phage dilution yielding an OD value of 1.5 (T_OD1.5).</p

Amino acid substitution of 8H5-reactive 12mer peptides.

<p> <i>133 distinct 12mer peptides reactive with 8H5 were identified from 5 sub-libraries of p125 as described in </i><a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0024144#pone-0024144-t001" target="_blank"><i>Table 1</i></a><i>.</i></p

Evaluation of ELISA produced with the 8H5 reactive12mer peptide (V-1b).

<p>In the top panel, microplates were coated with V-1b-HB core antigen (HBc-V-1b) were reacted with monoclonal antibodies specific for H5 molecule of H5N1 avian influenza virus (H5 mAbs), hepatitis E virus (HEV mAbs), hepatitis C virus (HCV mAb) and monoclonal antibodies specific for the HBV core antigen (HBV mAbs). In the bottom panel, these and also microplates coated with HBc alone were reacted with serum samples from 29 chicken infected with the H5N1 avian influenza virus and 8 control uninfected animals.</p

Data_Sheet_1_Impact of advanced liver fibrosis on atrial fibrillation recurrence after ablation in non-alcoholic fatty liver disease patients.docx

AimAdvanced liver fibrosis is independently associated with new onset of atrial fibrillation (AF). Non-invasive liver fibrosis scores are considered an effective strategy for assessing liver fibrosis. This study aimed to investigate the association between advanced liver fibrosis and AF recurrence after ablation in patients with non-alcoholic fatty liver disease (NAFLD).Materials and methodsA total of 345 AF patients with NAFLD who underwent de novo ablation between 2019 and 2020 at two large hospitals in China were included in this study. AF recurrence was defined as the occurrence of atrial arrhythmia for more than 30 s by electrocardiogram or 24 h Holter monitoring after the first 3 months of ablation. Predictive values of non-alcoholic fatty liver disease fibrosis score (NFS) and Fibrosis-4 (FIB-4) scores for AF burden and recurrence after ablation were assessed.ResultsAt the 1 year follow-up after ablation, 38.8% of patients showed recurrence. Patients with recurrence who had higher FIB-4 and NFS scores were more likely to have persistent AF and a duration of AF ≥ 3 years. In Kaplan–Meier analysis, patients with intermediate and high NFS and FIB-4 risk categories had a higher risk of AF recurrence. Compared to patients with the low risk, intermediate and high NFS, and FIB-4 risk were independently associated with AF recurrence in multivariate Cox regression analysis (high risk: NFS, hazard ratio (HR): 3.11, 95% confidence interval (CI): 1.68∼5.76, p ConclusionNFS and FIB-4 scores for advanced liver fibrosis are associated with AF burden. Advanced liver fibrosis is independently associated with AF recurrence following ablation. Advanced liver fibrosis might be meaningful in risk classification for patients after AF ablation.</p

The abnormal level of β-catenin affected AVBW closure.

<p>(A and C) a whole-mount view of the abdomen revealed that in severely affected Msx2-cre; β-catenin^Δex3 mutants, AVBW closure had not been completed at E16.5, at which stage the AVBW of normal littermates has already closed at the abdominal midline (black arrowheads). (B and D) Sagittal view of the abdominal center. Yellow arrowheads indicate the intestines. (E–H) At E14.5, when obvious umbilical ring structures were present in the abdomen of normal embryos, the AVBW was closed in severely affected Msx2-cre; β-catenin mutants (black arrowheads), as revealed by the enclosure of the intestines by the body wall (F and H). Green arrowheads indicate the body wall.</p