2 research outputs found
Diagnosis–Therapy Integrative Systems Based on Magnetic RNA Nanoflowers for Co-drug Delivery and Targeted Therapy
This study was to develop a codrug
delivery system for targeting
cancer therapy based on magnetic RNA nanoflowers (RNA NF). Compared
with traditional nucleic acid structure, convenient separation can
be achieved by introducing magnetic nanoparticle (MNP) into RNA NF.
Folic acid (FA) modified MNP/RNA NF (FA/MNP/RNA NF) was used as a
targeting nanocarrier with excellent biocompatibility to overcome
the nonselectivity of MNP/RNA NF. And then, anticancer drug doxorubicin
(DOX) and photosensitizer 5, 10, 15, 20-tetrakis (1-methylpyridinium-4-yl)
porphyrin (TMPyP<sub>4</sub>) binding with RNA NF were used as codrug
cargo models. RNA NF was first used for codrug delivery. So, imaging
fluorescent tags, target recognition element, and drug molecules were
all assembled together on the surface of MNP/RNA NF. The experimental
results suggested that the treatment efficacy of codrug delivery platform
(FA/MNP/RNA NF/D/T) was better than single-drug delivery platform
(FA/MNP/RNA NF/D). Besides, the FA/MNP/RNA NF was used as a probe
for cancer cell detection. The limit of detection was 50 HeLa cells.
In conclusion, the codrug delivery platform based on FA/MNP/RNA NF
was a promising approach for the intracellular quantification of other
biomolecules, as well as a diagnosis–therapy integrative system
Ti<sub>3</sub>C<sub>2</sub> MXene Nanosheet-Based Dual-Enzyme Cascade Reaction to Facilitate Dual-Stimulation-Mediated Breast Cancer Therapy
Starvation therapy mediated by glucose oxidase is a widely
used
therapeutic approach for tumor treatment, but it is limited by the
simultaneous drawbacks of weak therapeutic efficacy, nonspecificity,
and systemic toxicity. Thus, combination therapy was used to complement
the widely used therapeutic strategy for anticancer therapy. On the
basis of starvation therapy, we designed a catalytic model of nanosheets
with biological cascade enzymes synergizing with anticancer drugs.
In short, two cascade enzymes (glucose oxidase and horseradish peroxidase)
are covalently immobilized on Ti3C2 MXene nanosheet
and a cascade enzyme nanoreactor is formed by electrostatically adsorbing
positive charged DOX. Finally, the outer layer is coated with hyaluronic
acid. By combining glucose oxidase-mediated starvation therapy, photothermal
therapy, and chemotherapy, we have achieved the therapeutic effect
of “killing three birds with one stone” by combining
the dual stimulation response of endogenous and exogenous sources
to the tumor site. This method not only achieves the targeting of
cancer cells but also improves the systemic toxicity and reduced efficacy
of biological enzymes and realizes synergistic cancer therapy with
enhanced cascade reactions. It opens up a new path for the research
of nanomedicine