Examples of disruption of genes in human cell lines by ULtiMATE-engineered TALENs.

<p>(<b>A</b>, <b>D</b> and <b>G</b>) Partial sequences of <i>HBEGF</i>, <i>ANTXR1</i>, and <i>LRP1</i> genes in genome containing TALENs binding regions (overlined for TALEN^L and underlined for TALEN^R). Restriction enzyme cutting sites are highlighted in yellow. (<b>B</b>, <b>E</b> and <b>H</b>) Measurement of indel rates in TALENs-treated HeLa and HEK293T cells by restriction enzyme digestion. The uncleaved bands indicate potential indels. Both wild type and cells treated by TALENs targeting <i>ANTXR2</i> gene are used as controls. The percentage of uncleaved band (indicated by red arrow) was measured using ImageJ (<a href="http://rsbweb.nih.gov/ij/" target="_blank">http://rsbweb.nih.gov/ij/</a>). (<b>C</b>, <b>F</b> and <b>I</b>) Sequencing analysis of mutated alleles from 4-6 randomly selected TALENs clones (in HeLa cells). The TALENs binding sites (underlined) and restriction enzyme cutting sites (in yellow) are highlighted. Dashes and red letters indicate deletions and insertions, respectively.</p

Survival rate of septic mice after injection with baicalin.

<p>Baicalin improves survival of polymicrobial sepsis in CLP mice. In the bai group, mice were treated with intra-peritoneal baicalin (100 mg/kg) at 1 h, 6 h and 12 h after CLP. Normal saline of equal volume was administered in sham and CLP groups. Bai, baicalin group; CLP, CLP group. Survival analyses with log-rank test. ** <i>P</i><0.01.</p

The number of neutrophils in peritoneum in septic mice after administration of baicalin.

<p>Baicalin reduced the number of neutrophils in peritoneum. Peritoneal lavage fluid were harvested 24 h after CLP. Bai, baicalin group; CLP, CLP group. Data analyses with one-way ANOVA and Newman-Keuls. * <i>P</i><0.05.</p

Bacterial clearance in blood (A) and peritoneum (B) in septic mice after baicalin treatment.

<p>Baicalin enhanced bacterial clearance in blood (A) and peritoneum (B). Blood and peritoneal lavage fluid were harvested 24 h after CLP. Bai, baicalin group; CLP, CLP group. Data analyses with one-way ANOVA and Newman-Keuls. ** <i>P</i><0.01.</p

Histopathological changs of septic mice after baicalin treatment.

<p>The tissues were harvested 24 h after CLP for histopathologic examination using hematoxylin and eosin staining. Representative images from six animals per group were shown. Histopathological tests showed milder impairment in lung and liver after baicalin administration. A, After baicalin treatment, lung showed less neutrophil accumulation and alveolar destruction. B, Hepatocytes were protected and hepatic sinusoid was also preserved in baicalin treated mice. C, the severity of lung injury was scored as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0035523#s2" target="_blank">Materials and methods</a>. D, the severity of liver injury was scored as above. ** <i>P</i><0.01 vs. sham. ## <i>P</i><0.01 vs. CLP. Bai, baicalin group; CLP, CLP group.</p

Chest radiographic findings and progression of pneumonia in 6 patients with H7N9 infection.

<p>Case 1: Panels A, B, and C; Case 4: Panels D, E, and F; Case 2: Panels G, H, and I. Early stage (panels A, D, and G): Chest computed tomography and radiograph show progressive consolidation in the lungs; Progressing stage (panels B, E, and H): Chest computed tomography and radiograph show bilateral ground-glass opacity and consolidation; Final stage (panels C, F, and I): Chest computed tomography and radiograph show diffuse lesions and bilateral involvement of the lungs (white lungs) in panels C and F, subcutaneous emphysema with pneumomediastinum in panel F, and diffuse lesions are obviously seen in panel I.</p

A map of patient residences and the 2 neighboring markets where H7N9 was detected in poultry.

<p>A map of patient residences and the 2 neighboring markets where H7N9 was detected in poultry.</p