8925174 Raw data Biomed Research Inte.rar

miR-504 antagomir up-regulates NIS expression and improves the effect of 131 I therapy in thyroid cance

Comparison of 128-Slice Low-Dose Prospective ECG-Gated CT Scanning and Trans-Thoracic Echocardiography for the Diagnosis of Complex Congenital Heart Disease

<div><p>Objective</p><p>To compare prospective ECG-gated multi-slice computed tomography (MSCT) and trans-thoracic echocardiography (TTE) in the diagnosis of complex congenital heart disease (CHD).</p><p>Methods</p><p>This was a prospective study of consecutive patients with complex CHD (age <7 years) treated at a tertiary hospital between May 2013 and May 2015. All patients were imaged with TTE and prospective ECG-gated 128-slice spiral CT in the week before surgery. Effective radiation dose (ED) was calculated from volume CT dose index (CTDIvol) and dose length product (DLP). Image quality (5-point scale) was assessed independently by two radiologists. Using surgical findings as the reference, the diagnostic capabilities of MSCT and TTE were compared.</p><p>Results</p><p>Thirty-five patients (19 males) aged 1.59±1.58 years (range, 3 days to 74 months) were included. CTDIvol, DLP and ED were 0.90±0.24 mGy, 12.9±4.7 mGy∙cm and 0.64±0.21 mSv (range, 0.358–1.196 mSv), respectively. Image quality score was 4.3±0.5, and all images met the diagnostic requirements. The sensitivity, specificity, positive predictive value, and negative predictive value for diagnosing CHD were 97.2%, 99.8%, 99.0%, and 99.5%, respectively, for MSCT, and 90.6%, 99.8%, 99.0%, and 98.4%, respectively, for TTE. MSCT not only had a higher sensitivity than TTE overall (97.2% vs. 90.6%; P<0.05), but was much more sensitive for the diagnosis of extracardiac vascular abnormalities (92.0% vs. 68.0%; P<0.05).</p><p>Conclusion</p><p>128-slice low-dose prospective ECG-gated CT scanning has important clinical value in the diagnosis of complex CHD in children, complementing and extending the findings of TTE.</p></div

Construction and expression of a two-gene vector containing <i>NIS</i> and <i>EGFP</i>.

<p>(A) Schematic representation of the functional NIS gene and EGFP (green fluorescent protein reporter) gene in Lv-EF1α-NIS-IRES-EGFP. (B) Immunofluorescence showing that NIS and EGFP were strongly expressed in the CNE-2Z-NIS cells. (a: green color shows EGFP expression; b: red color shows NIS expression; c: a merged picture) (magnification, 400×). (C) qPCR analysis of NIS expression in CNE-2Z and CNE-2Z-NIS cells. NIS mRNA expression normalized to the GAPDH endogenous reference was detected by qRT-PCR. Results are expressed as means ± SD of three independent experiments. (D) Western blot analysis of NIS expression in CNE-2Z and CNE-2Z-NIS cells. NIS protein (~90 kDa) expression in CNE-2Z-NIS but not in CNE-2Z cells was analyzed by Western blot. GAPDH (~36 kDa) was used as an internal control (a: CNE-2Z-NIS; b: CNE-2Z). All experiments were performed in triplicate.</p

Baseline characteristics of the study participants and radiation dose analysis.

<p>Baseline characteristics of the study participants and radiation dose analysis.</p

Immunohistochemical staining.

<p>(A) Expression of NIS, caspase-3 and Ki-67 in CNE-2Z and CNE-2Z-NIS xenograft tumors treated with ¹³¹I or PBS (magnification, 200×). (B) Immunohistochemical analysis of protein NIS, caspase3 and Ki67. High expression of NIS protein was observed in the cells of the CNE-2Z-NIS xenografts with ¹³¹I or PBS treatment compared with the cells of the CNE-2Z xenografts with ¹³¹I or PBS treatment (<i>p</i><0.001). High expression of Caspase3 protein was observed in the cells of the CNE-2Z-NIS xenografts treated with ¹³¹I compared with the other three groups (<i>p</i><0.001), but low expression of Ki67 protein in the cells of the CNE-2Z-NIS xenografts treated with ¹³¹I was observed compared with the other three groups (<i>p</i><0.001). Data are present as means ± SD.</p

Comparison of multi-slice spiral computed tomography and trans-thoracic echocardiography for the diagnosis of congenital heart malformations identified by surgery.

<p>Comparison of multi-slice spiral computed tomography and trans-thoracic echocardiography for the diagnosis of congenital heart malformations identified by surgery.</p

Effect of Lv-EF1α-NIS-IRES-EGFP on the proliferation of CNE-2Z cells.

<p>CNE-2Z cells and CNE-2Z-NIS cells were subjected to the CCK-8 assay at 12, 24, 36, 48 and 72 h. Results are means ± SD of three independent experiments.</p

Representative multi-slice spiral computed tomography images from a male patient aged 3 years.

<p>A. Scanning parameters. CTDIvol, volume computed tomography dose index; DLP, dose length product (DLP). The effective radiation dose was calculated to be 0.018×2.3×25 = 1.035 mSv. B. Multiplanar reconstructed image showing a single ventricle, tricuspid valve atresia (white arrow), and a large atrial septal defect (black arrow). C. Maximum intensity projection showing the connection between the superior vena cava and right pulmonary artery (after surgery with the Glenn procedure), and primary pulmonary artery atresia (red arrow). D. Volume rendered image showing the features seen in C. LA, left atrium; PA, pulmonary artery; RA, right atrium; SV, single ventricle; SVC, superior vena cava.</p

Quantitative analysis of ¹²⁵I uptake <i>in vivo</i>.

<p>Conc values (μCi/mm³) of the xenograft tumors and organs after injection of ¹²⁵I (same time points as in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0116531#pone.0116531.g004" target="_blank">Fig. 4</a>).</p

Representative multi-slice spiral computed tomography images from a female patient aged 4 months.

<p>A. Scanning parameters. CTDIvol, volume computed tomography dose index; DLP, dose length product (DLP). The effective radiation dose was calculated to be 0.026 × 2.3 × 8 = 0.478 mSv. B-D. Multiplanar reconstructed images showing subvalvular aortic stenosis, with one side of the ventricular septum protruding into the left ventricular outflow tract (B, red arrow). It was observed in the aortic isthmus that one tubular channel was connected to the aorta (C, white arrow), and there was an atrial septal defect (D, black arrow). AO, aorta; DAO, descending aorta; LA, left atrium; LV, left ventricle; PA, pulmonary artery; RA, right atrium; RV, right ventricle.</p