Application of hyperbaric oxygen in bone tissue engineering : effect of hyperbaric oxygen treatment on bone marrow stem cells

and non-union of bony fractures has been proposed since 1966, little has been known about the effect of HBOT on bone marrow stem cells (BMSC). The aim of this study is to investigate the effect of HBO treatment on osteogenetic differentiation of BMSC and potential application in bone tissue engineering. Adhesive stromal cells harvested from bone marrow were characterized by mesenchymal differentiation potential, cell surface markers and their proliferation capacity. Mesenchymal stem cells, which demonstrated osteogenic, chondrogenic and adipogenic differentiation potential and expressed positively for CD 29, CD 44, CD 73, CD 90, CD 105, CD 166 and negatively for CD34 and CD 45, were selected and treated in a laboratory-scale HBO chamber using different oxygen pressures and exposure times. No obvious effect of HBO treatment on BMSC proliferation was noticed. However, cytotoxic effects of HBO were considerably less pronounced when cells were cultured in medium supplemented with 10% FBS in comparison to medium supplemented with 2% FCS, as was evaluated by WST-1 assay. Under HBO treatment, bone nodules were formed in three days, which was clearly revealed by Von Kossa staining. In contrasts, without HBO treatment, bone nodules were not detected until 9-12 days using the same inducing culture media. Calcium deposition was also significantly increased after three days of HBO treatments compared to no HBO treatment. In addition it was also found that oxygen played a direct role in the enhancement of BMSC osteogenic differentiation, which was independent of the effect of air pressure

Planck Constraints on Holographic Dark Energy

We perform a detailed investigation on the cosmological constraints on the holographic dark energy (HDE) model by using the Planck data. HDE can provide a good fit to Planck high-l (l>40) temperature power spectrum, while the discrepancy at l=20-40 found in LCDM remains unsolved in HDE. The Planck data alone can lead to strong and reliable constraint on the HDE parameter c. At 68% CL, we get c=0.508+-0.207 with Planck+WP+lensing, favoring the present phantom HDE at > 2sigma CL. Comparably, by using WMAP9 alone we cannot get interesting constraint on c. By combining Planck+WP with the BAO measurements from 6dFGS+SDSS DR7(R)+BOSS DR9, the H0 measurement from HST, the SNLS3 and Union2.1 SNIa data sets, we get 68% CL constraints c=0.484+-0.070, 0.474+-0.049, 0.594+-0.051 and 0.642+-0.066. Constraints can be improved by 2%-15% if we further add the Planck lensing data. Compared with the WMAP9 results, the Planck results reduce the error by 30%-60%, and prefer a phantom-like HDE at higher CL. We find no evident tension between Planck and BAO/HST. Especially, the strong correlation between Omegam h^3 and dark energy parameters is helpful in relieving the tension between Planck and HST. The residual chi^2_{Planck+WP+HST}-chi^2_{Planck+WP} is 7.8 in LCDM, and is reduced to 1.0 or 0.3 if we switch dark energy to the w model or the holographic model. We find SNLS3 is in tension with all other data sets; for Planck+WP, WMAP9 and BAO+HST, the corresponding Delta chi^2 is 6.4, 3.5 and 4.1, respectively. Comparably, Union2.1 is consistent with these data sets, but the combination Union2.1+BAO+HST is in tension with Planck+WP+lensing, corresponding to a Delta chi^2 8.6 (1.4% probability). Thus, it is not reasonable to perform an all-combined (CMB+SNIa+BAO+HST) analysis for HDE when using the Planck data. Our tightest self-consistent constraint is c=0.495+-0.039 obtained from Planck+WP+BAO+HST+lensing.Comment: 29 pages, 11 figures, 3 tables; version accepted for publication in JCA

Laspeyres Decomposition of Energy Intensity including Household-energy Factors

AbstractIn the previous study papers the change of the total energy intensity is decomposed to the structure factor and the efficiency factor, not considering the household energy factor, because GDP are not made only by household energy. Household energy is about ten percents in the total energy consumption and the household energy of unit GDP are always being decreased. In this paper the change of the total energy intensity is decomposed to the structure adjustment factor, the efficiency factor and the household energy factor. The result of decomposition shows the efficiency factor contribute most to the reduction of total energy intensity from 1985 to 2007, the household energy factor the next, and structure adjustment factor the last. The result of decomposition also explains the causation of the abnormal ascending of total energy intensity from 2002 to 2005

Diverse Consequences in Liver Injury in Mice with Different Autophagy Functional Status Treated with Alcohol

Alcoholic fatty liver disease is often complicated by other pathologic insults, such as viral infection or high-fat diet. Autophagy plays a homeostatic role in the liver but can be compromised by alcohol, high-fat diet, or viral infection, which in turn affects the disease process caused by these etiologies. To understand the full impact of autophagy modulation on alcohol-induced liver injury, several genetic models of autophagy deficiency, which have different levels of functional alterations, were examined after acute binge or chronic-plus-binge treatment. Mice given alcohol with either mode and induced with deficiency in liver-specific autophagy-related protein (Atg)-7 shortly after the induction of Atg7 deletion had elevated liver injury, indicating the protective role of autophagy. Constitutive hepatic Atg7–deficient mice, in which Atg7 was deleted in embryos, were more susceptible with chronic-plus-binge but not with acute alcohol treatment. Constitutive hepatic Atg5–deficient mice, in which Atg5 was deleted in embryos, were more susceptible with acute alcohol treatment, but liver injury was unexpectedly improved with the chronic-plus-binge regimen. A prolonged Atg deficiency may complicate the hepatic response to alcohol treatment, likely in part due to endogenous liver injury. The complexity of the relationship between autophagy deficiency and alcohol-induced liver injury can thus be affected by the timing of autophagy dysfunction, the exact autophagy gene being affected, and the alcohol treatment regimen

Protective Effect of FTY720 on Several Markers of Liver Injury Induced by Concanavalin A in Mice

AbstractBackground2-Amino-2-[2-(4-octylphenyl)ethyl] propane-1,3-diol hydrochloride (FTY720) is a novel agent with protective effect on several markers of liver injury. It is a chemical substance derived by modifying myriocin from the ascomycete Isaria sinclairii. It has been reported that FTY720 is able to treat autoimmune encephalomyelitis, renal cancer, asthma, and multiple sclerosis. More potent clinical applications of FTY720 need to be investigated.ObjectiveThe aim of this study was to evaluate the protective effect of FTY720 on several markers of experimental liver injury and to investigate the possible mechanism of action.MethodsConcanavalin A (Con A) at a dose of 15 mg/kg was intravenously. injected in mice, and 10 days before the Con A challenge, 1 mg/kg, 3 mg/kg, and 6 mg/kg of FTY720 were administered to mice. The liver injury was monitored biochemically by measuring serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) and tumor necrosis factor-α (TNF-α) levels. TNF-α and nuclear factor-κB (NF-κB) in liver tissue were detected by Western blot analysis.ResultsFTY720, when administered intragastrically for 10 days in mice with Con A–induced liver injury, dose-dependently reduced serum ALT and AST and TNF-α levels. The differences were statistically significant (P ≤ 0.05). It was also found that FTY720 decreases TNF-α and NF-κB protein expression in liver tissue.ConclusionsFTY720 is able to improve several markers of Con A–induced liver injury in mice, including serum ALT, serum AST, TNF-α, and NF-κB, which might be at least in part related to its ability to reduce TNF-α/NF-κB cascade activity

利培酮合并舒眠胶囊治疗精神分裂症的疗效及对患者糖、脂代谢的影响

Objective: To explore the curative effect and influence on glucose and lipid metabolism of risperidone with Shu Mian capsules in patients with schizophrenia .Methods: 128patients with chronic schizophrenia were randomly divided into the combined group (crisperidone combined with shu mian capsules group) and the single group (risperidone group ), each group was assessed the positive and negative syndrome scale (PANSS),the body mass index (BMI ) was measured and the blood glucose and blood lipid were detected before treatment and after 2,4,8 weekends respectively. After 4,8 weeks ,both the two groups were assessed by Wesconsin card sorting test ( WCST) curative effect and their cognitive function was evaluated .Results: The reacting time in the two groups were of no significantly difference , but compared with condition before treatment, cognitive function of the patients with schizophrenia was improved and their level of total cholesterol was significantly increased (P < 0.05) in the combined group. BMI, blood glucose and blood lipid were significantly increased in single group after 8weeks’ treatment (P < 0.05 or P < 0.01). Conclusion: Shu Mian capsules can lower negative symptoms and improve cognitive function of schizophrenia ,and the dverse reactions is mild. With therapeutic dose range, risperidone in combined group or single group can make equal reacting time, curative effect and affecting blood lipid metabolism in patients with schizophrenia. Single group shows more obvious influence on blood sugat and blood fat metabolismcan.目的  探讨利培酮合并舒眠胶囊治疗慢性精神分裂症的疗效及对患者糖、脂代谢的影响。方法  128例精神分裂症患者采用系统随机分为联合组（利培酮合并舒眠胶囊组）和单用组（利培酮组）。每组各64例分别于治疗前及治疗2、4、8周末采用阳性与阴性症状量表（PANSS）进行评分，并检测体质量指数（BMI）、血糖及血脂；4、8周末评价认知能力及疗效。结果  联合组和单用组起效时间比较差异无统计学意义；联合组能部分改善精神分裂症患者的认知能力，与治疗前相比8周未联合组总胆固醇明显增高（P＜0.05）；单用组BMI、血糖、总胆固醇和甘油三酯明显增高（P＜0.05 或P＜0.01）。结论  舒眠胶囊对精神分裂症阴性症状和认知功能有一定改善作用，不良反应轻微。利培酮治疗精神分裂症的剂量范围内联合组和单用组起效时间相当，但均可影响脂代谢；单用组对血糖、血脂代谢的影响更明显。 

Constraints on the primordial gravitational waves with variable sound speed from current CMB data

We make a comprehensive investigation of the observational effect of the inflation consistency relation. We focus on the general single-field inflation model with the consistency relation $r=-8c_s n_t$, and investigate the observational constraints of sound speed $c_s$ by using the Seven-Year WMAP data, the BICEP tensor power spectrum data, and the constraints on $f_{\rm NL}^{\rm equil.}$ and $f_{\rm NL}^{\rm orth.}$ from the Five-Year WMAP observations. We find that the constraints on the tensor-to-scalar ratio $r$ is much tighter if $c_s$ is small, since a large tilt $n_t$ is strongly constrained by the observations. We obtain $r<0.37, 0.27$ and 0.09 ($dn_s/d\ln k=0$) for $c_s$=1, 0.1 and 0.01 models at 95.4% confidence level. When taking smaller values of $c_s$, the positive correlation between $r$ and $n_s$ also leads to slightly tighter constraint on the upper bound of $n_s$, while the running of scalar spectral index $dn_s/d\ln k$ is generally unaffected. For the sound speed $c_s$, it is not well constrained if only the CMB power spectrum data is used, while the constraints are obtainable by taking $f_{\rm NL}^{\rm equil.}$ and $f_{\rm NL}^{\rm orth.}$ priors into account. With the constraining data of $f_{\rm NL}^{\rm equil.}$ and $f_{\rm NL}^{\rm orth.}$, we find that, $c_s\lesssim 0.01$ region is excluded at 99.7% CL, and the $c_s=1$ case (the single-field slow-roll inflation) is slightly disfavored at 68.3% CL. In addition, the inclusion of $f_{\rm NL}^{\rm equil.}$ and $f_{\rm NL}^{\rm orth.}$ into the analysis can improve the constraints on $r$ and $n_s$. We further discuss the implications of our constraints on the test of inflation models.Comment: 15 pages, 7 figures, updated versio