Species distribution of unigene BLASTX results against the NCBI-NR protein database with a cutoff E value <10⁻⁵.

<p>Different colors represent different species. Species with proportions of more than 1% are shown.</p

Expression profiles of genes involved in the primary metabolism pathway of viruliferous <i>Sogatella furcifera</i>.

<p>Expression profiles of genes involved in the primary metabolism pathway of viruliferous <i>Sogatella furcifera</i>.</p

The complete chloroplast genome sequence of <i>Zygophyllum brachypterum</i> (Zygophyllaceae) reveals its distinctive characteristics and evolutionary implication

Zygophyllum brachypterum Karelin & Kirilov belongs to Zygophyllaceae and is mainly distributed in the desert regions of Central Asia, Mongolia, and Northwest China. The species is valuable in exploring the adaptations of Zygophyllaceae plants to salt stress in ecological environments. In this study, we report the complete chloroplast (cp) genome of Z. brachypterum. The entire cp genome was 104590 bp in length, with a large single-copy region (LSC, 79170 bp), a small single-copy region (SSC, 16778 bp), and two inverted repeats (IRa/IRb) of 4321 bp each. A total of 106 genes were detected, among which seven were located in the IRs, and 65, 30, and 4 were protein-coding, tRNA, and rRNA genes, respectively. Notably, eleven genes encoding the subunits of NAD(P)H dehydrogenase complex (NDH) were absent. Phylogenetic analysis indicated that Z. brachypterum belonged to Zygophylloideae (Zygophyllaceae). Furthermore, it was closely related to Z. fabago and Z. kansuense.</p

Expression profiles of genes in the immune response pathway.

<p>Expression profiles of genes in the immune response pathway.</p

RT-PCR analysis and yeast two-hybrid screen of five virus-binding proteins.

<p>(A) Electrophoresis of gene amplified products was in 1% agarose gel. DNA markers were indicated on the left and genes on the top. (B) Yeast two-hybrid assay of protein-protein interactions between RSV-NCP and five virus-binding proteins (GAPDH3, RACK, RPL8, RPL5 and RPL7a). Yeast cotransformants were incubated on the selective medium (SD/–Ade/–His/–Leu/–Trp plus X-α-Gal) at 28°C for 4 d. 1: positive control; 2: negative control; 3: GAPDH3 & RSV-NCP; 4: RACK & RSV-NCP; 5: RPL8 & RSV-NCP; 6: RPL5 & RSV-NCP; 7: RPL7a & RSV-NCP.</p

Frequency of microsatellite classes in the transcriptome of <i>Sogatella furcifera</i>.

<p>(A) Summary of SSR analysis resulting from MISA software prediction. (B) The mono- to hexanucleotide microsatellite distributions in the transcriptome sequence of <i>S. furcifera</i>.</p

SDS-PAGE analysis and binding experiments with RSV particles of expression products of virus-binding proteins.

<p>(A) Expression products were separated by 12% SDS-PAGE. (B) Binding experiments with purified RSV particles by far-Western blot. (C) Binding experiments with RSV particles by DIBA. Lane 1, 8: relative molecular weight markers; lane 2, 9, 16: pET32a vector; lane 3, 10, 17: pET-GAPDH3; lane 4, 11, 18: pET-RACK; lane 5, 12, 19: pET-RPL8; lane 6, 13, 20: pET-RPL5; lane 7, 14, 21: pET-RPL7a; lane 15: <i>E. coli</i> BL21 (wt).</p

Expression profiles of genes in the ubiquitin-proteasome and cell cycle pathways.

<p>Expression profiles of genes in the ubiquitin-proteasome and cell cycle pathways.</p

Summary of differentially expressed genes between the two libraries.

<p>(A) Summary of the percent of differentially expressed genes in the Southern rice black-streaked virus infected <i>Sogatella furcifera</i>. (B) Fold change distribution of differentially expressed genes.</p

Summary statistics for <i>Sogatella furcifera</i> genes resulting from Illumina deep sequencing.

<p>Summary statistics for <i>Sogatella furcifera</i> genes resulting from Illumina deep sequencing.</p