Residues at the hydrophobic interface between AscE and AscG_1–61.

<p>Ribbon representation of the AscE-AscG_1–61 crystal structure showing side chains of residues involved in the hydrophobic interface between AscE (blue) and AscG_1–61 (red), at two different views.</p

Thermal denaturation of AscE-AscG, AscE-AscG_1–61, AscE-AscG-AscF and AscE-AscG-AscF_53–87 complexes monitored by Far-UV CD.

<p>Thermal denaturation of the AscE-AscG_1–61 (closed circle), AscE-AscG full length (open circle), AscE-AscG-AscF_53–87 (closed square) and AscE-AscG-AscF full length (open square) complexes monitored by Far-UV CD at 220 nm from 10°C to 85°C.</p

Superposition of the structure AscE-AscG_1–61 with PscE-PscF-PscG and YscE-YscF-YscG.

<p>The crystal structure of AscE-AscG_1–61 was superpositioned with (A) PscE-PscF-PscG or (B) YscE-YscF-YscG at two different views. The AscE and AscG_1–61 proteins are colored blue and red, respectively. The E, F and G proteins in the PscE-PscF-PscG or YscE-YscF-YscG complexes are colored magenta, green and cyan, respectively.</p

Surface diagram of the AscE-AscG_1–61 complex and predicted AscF interacting residues.

<p>Surface diagram of the AscE-AscG_1–61 complex showing the tight interaction between AscE (blue) and AscG_1–61 (white), at two different views. The residues on AscG_1–61 predicted to interact with AscF based on the crystal structures of PscE-PscF-PscG and YscE-YscF-YscG are colored red. The surface diagrams were generated using the software Chimera <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0019208#pone.0019208-Pettersen1" target="_blank">[44]</a>.</p

Crystallographic data and Refinement Statistics.

a<p>R_sym = |I_i−<i>|/|I_i| where I_i is the intensity of the i^th measurement, and <i> is the mean intensity for that reflection.</i></i></p><i><i>b<p>R_work = |F_obs−F_calc|/|F_obs| where F_calc and F_obs are the calculated and observed structure factor amplitudes respectively.</p>c<p>R_free = as for R_work, but for 10.0% of the total reflections chosen at random and omitted from refinement.</p></i></i