Ultraviolet-Visible (UV) Absorption Spectrum of BC-EDTA.

<p>Ultraviolet-Visible (UV) Absorption Spectrum of BC-EDTA.</p

Outline of labeling bare particles with BC-EDTA.

<p>Outline of labeling bare particles with BC-EDTA.</p

Regression analysis result of BC-EDTA/Eu³⁺-nanosphere based TrFIA and DELFIA.

<p>Regression analysis result of BC-EDTA/Eu³⁺-nanosphere based TrFIA and DELFIA.</p

Functional sensitivity of BC-EDTA/Eu³⁺-nanosphere.

<p>^a Mean value of net fluorescence counts (n = 10).</p><p>Functional sensitivity of BC-EDTA/Eu³⁺-nanosphere.</p

Relationship between fluorescence intensity of nanosphere and coating times.

<p>BC-EDTA/Eu³⁺-nanosphere was diluted in 0.05 mol/L carbonate buffer, pH9.5, with the concentration of 0.01%.</p

Synthesis of BC-EDTA.

<p>Synthesis of BC-EDTA.</p

Calibration curves for HBsAg.

<p>Calibration curves for HBsAg.</p

Ab Initio Emulsion and Miniemulsion Polymerization of Styrene Mediated by a Cyclohexenyl-Functionalized Amphiphilic RAFT Agent

We designed and used a cyclohexenyl-functionalized amphiphilic macromolecular reversible addition–fragmentation chain transfer (macro-RAFT) agent as surfactant and polymerization mediator for (mini)­emulsion polymerization of styrene (St), aiming at synthesizing nanoparticles with vinyl-enriched surface and well-defined polymer chains. Unexpectedly, the pendent cyclohexenyl units in the macro-RAFT agent were found to undergo significant copolymerization with St exclusively during the nucleation period in the ab initio emulsion polymerization, leading to the consumption of cyclohexenyl groups, positive deviation of molecular weights, and broad molecular weight distributions. By contrast, in miniemulsion polymerization, the radical polymerization exhibited superb selectivity toward St monomers relative to the pendent cyclohexene vinyl units just as in the solution polymerization. The obtained polymer chains retained designed molecular weights, low polydispersity indexes, and quantitative cyclohexenyl functionalities confined to the particle surface. Thus, PSt particles with vinyl-enriched surface were obtained via miniemulsion polymerization

DataSheet7_Integrative proteomics and metabolomics approach to identify the key roles of icariin-mediated protective effects against cyclophosphamide-induced spermatogenesis dysfunction in mice.CSV

The alkylating antineoplastic agent cyclophosphamide (CP) is known to be toxic to the male reproductive system, but there are no effective prevention or treatment options. The flavonoid icariin (ICA), which is used in Chinese medicine, has been shown to have a number of biological functions, including testicular protection. The current study looked into the protective effects of ICA in preventing CP-induced spermatogenesis dysfunction. The current study looked into the role of ICA in preventing testicular dysfunction caused by CP. For 5 days, healthy adult mice were given saline or a single dose of CP (50 mg/kg) intraperitoneally (i.p). For the next 30 days, mice were given ICA (80 mg/kg) by gavage. Animals were euthanized 12 h after receiving ICA, and testes were removed for biochemical, histopathological, sperm evaluation, and transmission electron microscope analysis (TEM). We also investigated the potential biological effects of ICA on CP-induced spermatogenesis dysfunction in mice using an integrated proteomic and metabolomic approach. The levels of 8309 proteins and 600 metabolites were measured. The majority of the differential proteins and metabolites were found to be enriched in a variety of metabolic pathways, including the PI3K-Akt signaling pathway, necroptosis, the mTOR signaling pathway, glycerophospholipid metabolism, and ABC transporters, implying that ICA may have molecular mechanisms that contribute to CP-induced spermatogenesis dysfunction in the testis. Taken together, these findings show that ICA effectively reduces testis injury, implying that ICA may have a role in male infertility preservation.</p

DataSheet5_Integrative proteomics and metabolomics approach to identify the key roles of icariin-mediated protective effects against cyclophosphamide-induced spermatogenesis dysfunction in mice.XLS

The alkylating antineoplastic agent cyclophosphamide (CP) is known to be toxic to the male reproductive system, but there are no effective prevention or treatment options. The flavonoid icariin (ICA), which is used in Chinese medicine, has been shown to have a number of biological functions, including testicular protection. The current study looked into the protective effects of ICA in preventing CP-induced spermatogenesis dysfunction. The current study looked into the role of ICA in preventing testicular dysfunction caused by CP. For 5 days, healthy adult mice were given saline or a single dose of CP (50 mg/kg) intraperitoneally (i.p). For the next 30 days, mice were given ICA (80 mg/kg) by gavage. Animals were euthanized 12 h after receiving ICA, and testes were removed for biochemical, histopathological, sperm evaluation, and transmission electron microscope analysis (TEM). We also investigated the potential biological effects of ICA on CP-induced spermatogenesis dysfunction in mice using an integrated proteomic and metabolomic approach. The levels of 8309 proteins and 600 metabolites were measured. The majority of the differential proteins and metabolites were found to be enriched in a variety of metabolic pathways, including the PI3K-Akt signaling pathway, necroptosis, the mTOR signaling pathway, glycerophospholipid metabolism, and ABC transporters, implying that ICA may have molecular mechanisms that contribute to CP-induced spermatogenesis dysfunction in the testis. Taken together, these findings show that ICA effectively reduces testis injury, implying that ICA may have a role in male infertility preservation.</p