1 research outputs found
Digital Single Virus Electrochemical Enzyme-Linked Immunoassay for Ultrasensitive H7N9 Avian Influenza Virus Counting
Electrochemistry has been widely
used to explore fundamental properties
of single molecules due to its fast response and high specificity.
However, the lack of efficient signal amplification strategies and
quantitative method limit its clinical application. Here, we proposed
a digital single virus electrochemical enzyme-linked immunoassay (digital
ELISA) for H7N9 avian influenza virus (H7N9 AIV) counting by integration
of digital analysis, bifunctional fluorescence magnetic nanospheres
(bi-FMNs) with monolayer gold nanoparticles (Au NPs) modified microelectrode
array (MA). Bi-FMNs are fabricated by coimmobilizing polyclonal antibody
(pAb) and alkaline phosphatase (ALP). At most, one target will be
captured per bi-FMNs by controlling the proportion of bi-FMNs to target
concentrations (≥5:1). The introduction of digital analysis
can solve signal fluctuation and the reliability of single virus detection,
enabling the digital ELISA to be sensitively and accurately applied
for H7N9 AIV detection with a low detection limit of 7.8 fg/mL, which
is greatly promising in single biomolecular detection, early diagnosis
of disease, and practical application