Investigation into the Diversity of the Asia-Pacific Region and Scope for Collaboration Report on Research Project

千葉大学人文社会科学研究科研究プロジェクト報告書第200

Elegant Chemistry to Directly Anchor Intact Saccharides on Solid Surfaces Used for the Fabrication of Bioactivity-Conserved Saccharide Microarrays

An easy chemical strategy was proposed and used to establish a method for direct anchoring of intact saccharides on solid surfaces with well conserved bioaffinity. The anchoring was achieved by temperature-modulated stepwise reactions with cyanuric chloride as a key linker, and was successfully applied to the fabrication of saccharide chips. To demonstrate, 15 intact reducing and nonreducing saccharides with various molecular sizes were dotted on a cyanuric-chloride-modified chip (1.0 × 1.0 cm²) and made to react with lectins. As expected, the anchored saccharides were capable of recognizing their target lectins, and more exciting were the perfect conservation of the specific recognizing ability of the anchored monosaccharides such as mannose, glucose, and even fructose (interacting only weakly with concanavalin A). This conservation was ascribed to the maintenance of the original structure (especially the anomeric configuration) of saccharides after immobilization and to the allowance of the anchored saccharides to rotate with and/or on the scaffold of cyanuric chloride, which makes them easily adapt to the recognition-preferred spatial position. The expected linkage of saccharides on cyanuric chloride and the maintenance of their anomeric configuration were characterized by mass spectrometry and nuclear magnetic resonance, respectively. The new method can be highlighted not only by its conservation of saccharide bioaffinity and universal applicability but also by its merits of easy manipulation or facile control of the reactions and cost-effectiveness due to the use of extremely cheap cyanuric chloride

Physical Activity and Risks of Esophageal and Gastric Cancers: A Meta-Analysis

<div><p>Background</p><p>The incidence of esophageal and gastric cancer has been increasing rapidly worldwide in recent years, although the reason for this increase is unclear. Here, a statistical synthesis of studies that evaluated the association between physical activity, a well-known protecting factor against death and other chronic diseases, and the risk of esophageal and gastric cancer was performed.</p><p>Methods</p><p>Potentially suitable studies were identified using Medline and Embase. The reference lists of all included articles and those of several recent reviews were searched manually. Studies were included if they (1) were published as case-control or cohort studies evaluating the association between physical activity and risk of esophageal or gastric cancer; and (2) reported point estimates (i.e., risk ratios, odds ratios) and measures of variability (i.e., 95% confidence intervals [CIs]) for physical activity and risk of esophageal or gastric cancer.</p><p>Results</p><p>Fifteen studies were identified (7 cohorts, 8 case-controls; 984 esophageal and 7,087 gastric cancers). Collectively, they indicated that the risk of gastric cancer was 13% lower among the most physically active people than among the least active people (RR = 0.87, 95% confidence interval [CI] = 0.78 to 0.97) and that of esophageal cancer was 27% lower (RR = 0.73, 95% CI = 0.56 to 0.97).</p><p>Conclusions</p><p>Pooled results from observational studies support a protective effect of physical activity against both esophageal and gastric cancer.</p></div

Surface Plasmon Resonance Imaging Detection of Sub-femtomolar MicroRNA

MicroRNA (miRNA) is a promising new type of biomarkers but at a low fM level and hard to be analyzed. Herein proposed is an innovated surface plasmon resonance imaging (SPRi) method merged with a novel in-plane and vertical signal amplification strategy, that is, orthogonal signal amplification to enable a direct determination of sub-fM miRNA-15a (a multiple tumor diagnostic biomarker). The core idea is to add more mass on a target sample spot first along the surficial direction, then upward from the surface. In detection of miRNA, this was realized by coupling a miRNA-initiated surficial cyclic DNA–DNA hybridization reaction with a DNA-initiated upward cyclic polymerization reaction. A perfect SPRi sensing chip with isolated gold islands bordered by hydrophobic CYTOP was fabricated and used to obtain high-quality chip with low fabrication difficulty. As a result, SPRi contrast largely increases, able to reach a limit of detection and limit of quantification down to 0.56 and 5fM for miRNA-15a, about 10⁷-fold improvement of sensitivity compared with a common SPRi detection. The method could quantify standard miRNA-15a spiked in human serum with an ideal recovery ranging from 98.6% to 104.9% and was validated to be applicable to the direct determination of miRNA-15a in healthy and cancer human serums. The orderly and controllable in situ sensitizing strategy is powerful and readily extendable to detection of other miRNAs

The behaviors of bat swarms.

<p>The behaviors of bat swarms.</p

Main characteristics of the studies in the primary meta-analysis that have investigated the association between PA and GC and EC.

<p>Main characteristics of the studies in the primary meta-analysis that have investigated the association between PA and GC and EC.</p

Fitness curves of mmAP

<p><b> mmVAR over the simulation.</b></p

Stepwise Deprotonation of Magnetite-Supported Gallic Acid Modulates Oxidation State and Adsorption-Assisted Translocation of Hexavalent Chromium

Recently, a synergistic strategy involving reduction of carcinogenic Cr­(VI) into less toxic Cr­(III) followed by Cr­(III) adsorption and subsequent separation by surface-engineered magnetite nanoparticles has emerged as a promising alternative to address the environmental hazards associated with Cr­(VI)-contaminated water. Despite several previous attempts exploiting this synergy, modulating the oxidation state and translocation of Cr­(VI) with high spatiotemporal precision remains a major challenge. Here, we report how Cr­(VI) responds accordingly in a well-defined manner to deprotonation of gallic acid covalently immobilized on magnetite nanoparticles, which proceeds through a fixed spatial sequence of distinct stages. To the best of our knowledge, this proof-of-principle study, for the first time, demonstrates that accurate spatiotemporal control over the cascading reduction–adsorption process of Cr­(VI) by magnetic adsorbents is feasible, which provides guidance for rational design of more exquisite, magnetite-supported surfaces, where a predictable, and hence controllable, synergy can manifest for Cr­(VI) detoxification

Impacts of five coupled variables on in 3D figures.

<p>Impacts of five coupled variables on in 3D figures.</p

Framework of computational intelligence-aided design.

<p>Framework of computational intelligence-aided design.</p