2 research outputs found
New Horizons of Covalent Complex of Plant-Derived Recombinant Human Lactoferrin (OsrhLF) Combined with Different Polyphenols: Formation, Physicochemical Properties, and Gastrointestinal Fate
Four typical dietary polyphenols ((−)-epigallocatechin
gallate (EGCG), quinic acid (QA), caffeic acid (CA), and ferulic acid
(FA)) were covalently prepared with rice recombinant human lactoferrin
(OsrhLF) and bovine lactoferrin (bLF), and their structure and physicochemical
properties were investigated, different lycopene emulsions were made
by ultrasonic emulsification to analyze gastrointestinal fate. The
results indicated that the covalent modification polyphenols changed
the secondary/tertiary structure of LF, significantly improving the
surface hydrophilicity, thermal stability, and antioxidant activity
of LF. Compared with the bLF group, the OsrhLF group was more hydrophilic
and the thermal denaturation temperature of the OsrhLF-CA reached
104.4 °C. LF–polyphenol emulsions significantly enhanced
the photochemical stability and bioavailability of lycopene and achieved
effective encapsulation and protection of lycopene compared to free
lycopene, and the OsrhLF–EGCG reached 58.94% lycopene bioavailability.
In short, OsrhLF does not differ much from bLF in terms of physicochemical
properties and has a strong potential in the field of dietary supplements
MOESM1 of Dynamic and specific immune responses against multiple tumor antigens were elicited in patients with hepatocellular carcinoma after cell-based immunotherapy
Additional file 1. Additional figures and tables