1 research outputs found
Tunable Physicomechanical and Drug Release Properties of In Situ Forming Thermoresponsive Elastin-like Polypeptide Hydrogels
With the continued advancement in the design and engineering
of
hydrogels for biomedical applications, there is a growing interest
in imparting stimuli-responsiveness to the hydrogels in order to control
their physicomechanical properties in a more programmable manner.
In this study, an in situ forming hydrogel is developed by cross-linking
alginate with an elastin-like polypeptide (ELP). Lysine-rich ELP synthesized
by recombinant DNA technology is reacted with alginate presenting
an aldehyde via Schiff base formation, resulting in facile hydrogel
formation under physiological conditions. The physicomechanical properties
of alginateāELP hydrogels can be controlled in a wide range
by the concentrations of alginate and ELP. Owing to the thermoresponsive
properties of the ELP, the alginateāELP hydrogels undergo swelling/deswelling
near the physiological temperature. Taking advantage of these highly
attractive properties of alginateāELP, drug release kinetics
were measured to evaluate their potential as a thermoresponsive drug
delivery system. Furthermore, an ex vivo model was used to demonstrate
the minimally invasive tissue injectability