Interactive effects of light, leaf temperature, CO 2 and O 2 on photosynthesis in soybean

A biochemical model of C 3 photosynthesis has been developed by G.D. Farquhar et al. (1980, Planta 149, 78–90) based on Michaelis-Menten kinetics of ribulose-1,5-bisphosphate (RuBP) carboxylase-oxygenase, with a potential RuBP limitation imposed via the Calvin cycle and rates of electron transport. The model presented here is slightly modified so that parameters may be estimated from whole-leaf gas-exchange measurements. Carbon-dioxide response curves of net photosynthesis obtained using soybean plants ( Glycine max (L.) Merr.) at four partial pressures of oxygen and five leaf temperatures are presented, and a method for estimating the kinetic parameters of RuBP carboxylase-oxygenase, as manifested in vivo, is discussed. The kinetic parameters so obtained compare well with kinetic parameters obtained in vitro, and the model fits to the measured data give r 2 values ranging from 0.87 to 0.98. In addition, equations developed by J.D. Tenhunen et al. (1976, Oecologia 26, 89–100, 101–109) to describe the light and temperature responses of measured CO 2 -saturated photosynthetic rates are applied to data collected on soybean. Combining these equations with those describing the kinetics of RuBP carboxylase-oxygenase allows one to model successfully the interactive effects of incident irradiance, leaf temperature, CO 2 and O 2 on whole-leaf photosynthesis. This analytical model may become a useful tool for plant ecologists interested in comparing photosynthetic responses of different C 3 plants or of a single species grown in contrasting environments.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/47469/1/425_2004_Article_BF00395048.pd

Reducing the environmental impact of surgery on a global scale: systematic review and co-prioritization with healthcare workers in 132 countries

Background Healthcare cannot achieve net-zero carbon without addressing operating theatres. The aim of this study was to prioritize feasible interventions to reduce the environmental impact of operating theatres. Methods This study adopted a four-phase Delphi consensus co-prioritization methodology. In phase 1, a systematic review of published interventions and global consultation of perioperative healthcare professionals were used to longlist interventions. In phase 2, iterative thematic analysis consolidated comparable interventions into a shortlist. In phase 3, the shortlist was co-prioritized based on patient and clinician views on acceptability, feasibility, and safety. In phase 4, ranked lists of interventions were presented by their relevance to high-income countries and low–middle-income countries. Results In phase 1, 43 interventions were identified, which had low uptake in practice according to 3042 professionals globally. In phase 2, a shortlist of 15 intervention domains was generated. In phase 3, interventions were deemed acceptable for more than 90 per cent of patients except for reducing general anaesthesia (84 per cent) and re-sterilization of ‘single-use’ consumables (86 per cent). In phase 4, the top three shortlisted interventions for high-income countries were: introducing recycling; reducing use of anaesthetic gases; and appropriate clinical waste processing. In phase 4, the top three shortlisted interventions for low–middle-income countries were: introducing reusable surgical devices; reducing use of consumables; and reducing the use of general anaesthesia. Conclusion This is a step toward environmentally sustainable operating environments with actionable interventions applicable to both high– and low–middle–income countries

Sustained-release ganciclovir implant as prophylaxis for cytomegalovirus retinitis in a child undergoing bone marrow transplantation

10.1038/eye.2013.81Eye (Basingstoke)277890-891EYEE

Real-time quantitative polymerase chain reaction (RO-PCR) using the LightCycler: a rapid, high-throughput method for detecting and quantifying fusion transcripts in childhood leukaemias for disease stratification and prognostication.

Annals of the Academy of Medicine, Singapore325 Supp

In vitro drug sensitivity and expression profiling for disease prognostication in childhood acute lymphoblastic leukaemia (ALL): an exploratory model using cell lines.

Annals of the Academy of Medicine, Singapore335 Supp

Ambulatory care of central venous Hickman's catheters can be successfully and safely carried out by parents at home: results of a patient education programme.

Annals of the Academy of Medicine, Singapore325 Supp

Simple rules underlying gene expression profiles of more than six subtypes of acute lymphoblastic leukemia (ALL) patients

10.1093/bioinformatics/19.1.71Bioinformatics19171-78BOIN

Double deletions of glutathione S-transferase genes (GSTM1 and GSTT1) reduce the risk of early relapse in childhood B-lineage acute lymphoblastic leukaemia (ALL).

Annals of the Academy of Medicine, Singapore335 Supp

Both gram positive and gram negative organisms complicate neutropaenic sepsis in children with leukemia. A unit audit study and recommendation.

Annals of the Academy of Medicine, Singapore325 SupplS37-3

Escherichia coli-asparaginase (Elspar) is superior to Erwinia-asparaginase (Erwinase) in childhood acute lymphoblastic leukaemia (ALL) induction--an early response study using minimal residual disease (MRD) markers.

Annals of the Academy of Medicine, Singapore335 Supp