Repeatability of a Short Food Frequency Questionnaire to Assess Calcium Intake in Older Australians

Objective. To assess the repeatability of the validated Flinders Calcium Food Frequency Questionnaire (FFQCA) for estimating dietary calcium intake in a sample of community dwelling older Australians. Methods. A test-retest repeatability study involving 100 subjects (≥65 years) living independently in metropolitan Adelaide, Australia. Estimates of daily calcium intake from the first (FFQCA1) and repeated administration (FFQCA2) were calculated from two versions (35-item and 15-item) of the FFQCA. The intraclass correlation coefficient (ICC) was used to assess the repeatability. Results. Moderate and comparable ICC values (r = 0.5, r = 0.6) were found across the two versions of the FFQCA. Conclusion. Both FFQCA versions demonstrated moderate repeatability, supporting the results of previous studies

Public perception of e-learning in Singapore.

This project explores the general public perception of E-learning in Singapore

Information access

Objective. To assess the repeatability of the validated Flinders Calcium Food Frequency Questionnaire (FFQ CA ) for estimating dietary calcium intake in a sample of community dwelling older Australians. Methods. A test-retest repeatability study involving 100 subjects (≥65 years) living independently in metropolitan Adelaide, Australia. Estimates of daily calcium intake from the first (FFQ CA1 ) and repeated administration (FFQ CA2 ) were calculated from two versions (35-item and 15-item) of the FFQ CA . The intraclass correlation coefficient (ICC) was used to assess the repeatability. Results. Moderate and comparable ICC values (r = 0.5, r = 0.6) were found across the two versions of the FFQ CA . Conclusion. Both FFQ CA versions demonstrated moderate repeatability, supporting the results of previous studies

Prevalence and incidence of stroke, white matter hyperintensities, and silent brain infarcts in patients with chronic heart failure: A systematic review, meta-analysis, and meta-regression

10.3389/fcvm.2022.967197FRONTIERS IN CARDIOVASCULAR MEDICINE

Deterministic Restriction on Pluripotent State Dissolution by Cell-Cycle Pathways

SummaryDuring differentiation, human embryonic stem cells (hESCs) shut down the regulatory network conferring pluripotency in a process we designated pluripotent state dissolution (PSD). In a high-throughput RNAi screen using an inclusive set of differentiation conditions, we identify centrally important and context-dependent processes regulating PSD in hESCs, including histone acetylation, chromatin remodeling, RNA splicing, and signaling pathways. Strikingly, we detected a strong and specific enrichment of cell-cycle genes involved in DNA replication and G2 phase progression. Genetic and chemical perturbation studies demonstrate that the S and G2 phases attenuate PSD because they possess an intrinsic propensity toward the pluripotent state that is independent of G1 phase. Our data therefore functionally establish that pluripotency control is hardwired to the cell-cycle machinery, where S and G2 phase-specific pathways deterministically restrict PSD, whereas the absence of such pathways in G1 phase potentially permits the initiation of differentiation

Klf2 Is an Essential Factor that Sustains Ground State Pluripotency

The maintenance of mouse embryonic stem cells (mESCs) requires LIF and serum. However, a pluripotent “ground state,” bearing resemblance to preimplantation mouse epiblasts, can be established through dual inhibition (2i) of both prodifferentiation Mek/Erk and Gsk3/Tcf3 pathways. While Gsk3 inhibition has been attributed to the transcriptional derepression of Esrrb, the molecular mechanism mediated by Mek inhibition remains unclear. In this study, we show that Krüppel-like factor 2 (Klf2) is phosphorylated by Erk2 and that phospho-Klf2 is proteosomally degraded. Mek inhibition hence prevents Klf2 protein phosphodegradation to sustain pluripotency. Indeed, while Klf2-null mESCs can survive under LIF/Serum, they are not viable under 2i, demonstrating that Klf2 is essential for ground state pluripotency. Importantly, we also show that ectopic Klf2 expression can replace Mek inhibition in mESCs, allowing the culture of Klf2-null mESCs under Gsk3 inhibition alone. Collectively, our study defines the Mek/Erk/Klf2 axis that cooperates with the Gsk3/Tcf3/Esrrb pathway in mediating ground state pluripotency.ASTAR (Agency for Sci., Tech. and Research, S’pore

dvdres-oct-2017-00153-File005 – Supplemental material for The association of serum creatinine and estimated glomerular filtration rate variability with diabetic retinopathy in Asians with type 2 diabetes: A nested case–control study

<p>Supplemental material, dvdres-oct-2017-00153-File005 for The association of serum creatinine and estimated glomerular filtration rate variability with diabetic retinopathy in Asians with type 2 diabetes: A nested case–control study by Xiao Zhang, Neelam Kumari, Serena Low, Keven Ang, Darren Yeo, Lee Ying Yeoh, Allen Liu, Pek Yee Kwan, Wern Ee Tang, Subramaniam Tavintharan, Chee Fang Sum and Su Chi Lim in Diabetes & Vascular Disease Research</p

Prognostic Outcomes in Acute Myocardial Infarction Patients Without Standard Modifiable Risk Factors: A Multiethnic Study of 8,680 Asian Patients

10.3389/fcvm.2022.869168FRONTIERS IN CARDIOVASCULAR MEDICINE

Effects of Sodium-Glucose Cotransporter 2 on Amputation Events: A Systematic Review and Meta-Analysis of Randomized-Controlled Trials.

10.1159/000520903Pharmacology107123-13

One-year outcomes of patients with ST-segment elevation myocardial infarction during the COVID-19 pandemic

10.1007/s11239-021-02557-6JOURNAL OF THROMBOSIS AND THROMBOLYSI