14 research outputs found
Hierarchical Porous Carbons from Poly(methyl methacrylate)/Bacterial Cellulose Composite Monolith for High-Performance Supercapacitor Electrodes
This study
deals with hierarchical porous carbons from bacterial cellulose (BC),
having a layered structure for high-performance application, such
as supercapacitor electrodes, fabricated from a composite monolith
with unique microscopic/macroscopic morphology. A polyÂ(methyl methacrylate)
(PMMA)/BC composite monolith was first synthesized by thermally induced
phase separation using ethanol and deionized water as solvents, where
BC acts as the main carbon source as well as matrix and PMMA acts
as the activator source producing the necessary activation material.
Scanning electron microscopy analysis showed that a monolithic skeleton
of PMMA was loaded uniformly on the nanofibers of BC to form a three-dimensional
entangled structure of the PMMA skeleton and BC nanofibers, as observed
in the microscopic view. Furthermore, the macroscopic two-dimensional
layered structure of BC remained in the as-obtained composite. The
specific surface area, structural features, and thermal stability
were investigated by Brunauer–Emmett–Teller, X-ray diffraction,
and thermogravimetric analysis studies. The resulting PMMA/BC composite
was carbonized and activated by KOH at 850 °C. The electrochemical
properties were characterized by cyclic voltammetry, galvanostatic
charge–discharge, and electrochemical impedance spectroscopy
showing that the carbonization product of the composite displayed
a high specific capacitance of 266 F g<sup>–1</sup> at a current
density of 0.50 A g<sup>–1</sup> and the energy density reached
a maximum of 23.6 W h kg<sup>–1</sup> at a power density of
200 W kg<sup>–1</sup>. Moreover, 95% of the capacitance was
retained after 10,000 charge–discharge cycles, which implies
exceptionally high cyclic stability. This compatible and excellent
electrochemical performance of the composite, in terms of the energy
density and capacitance retention, can be contributed to the characteristic
porous structure of the precursor composite monolith. The present
research delineates a new approach to fabricate high-performance supercapacitor
materials and low-cost energy storage devices from inexpensive bioresources
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Two-Dimensional MoS<sub>2</sub>‑Based Zwitterionic Hydrophilic Interaction Liquid Chromatography Material for the Specific Enrichment of Glycopeptides
Mass spectrometry
(MS)-based glycoproteomics research requires
highly efficient sample preparation to eliminate interference from
non-glycopeptides and to improve the efficiency of glycopeptide detection.
In this work, a novel MoS<sub>2</sub>/Au-NP (gold nanoparticle)–L-cysteine
nanocomposite was prepared for glycopeptide enrichment. The two-dimensional
(2D) structured MoS<sub>2</sub> nanosheets served as a matrix that
could provide a large surface area for immobilizing hydrophilic groups
(such as L-cysteine) with low steric hindrance between the materials
and the glycopeptides. As a result, the novel nanomaterial possessed
an excellent ability to capture glycopeptides. Compared to commercial
zwitterionic hydrophilic interaction liquid chromatography (ZIC-HILIC)
materials, the novel nanomaterials exhibited excellent enrichment
performance with ultrahigh selectivity and sensitivity (approximately
10 fmol), high binding capacity (120 mg g<sup>–1</sup>), high
enrichment recovery (more than 93%), satisfying batch-to-batch reproducibility,
and good universality for glycopeptide enrichment. In addition, its
outstanding specificity and efficiency for glycopeptide enrichment
was confirmed by the detection of glycopeptides from an human serum
immunoglobulin G (IgG) tryptic digest in quantities as low as a 1:1250
molar ratio of IgG tryptic digest to bovine serum albumin tryptic
digest. The novel nanocomposites were further used for the analysis
of complex samples, and 1920 glycopeptide backbones from 775 glycoproteins
were identified in three replicate analyses of 50 μg of proteins
extracted from HeLa cell exosomes. The resulting highly informative
mass spectra indicated that this multifunctional nanomaterial-based
enrichment method could be used as a promising tool for the in-depth
and comprehensive characterization of glycoproteomes in MS-based glycoproteomics
Two-Dimensional MoS<sub>2</sub>‑Based Zwitterionic Hydrophilic Interaction Liquid Chromatography Material for the Specific Enrichment of Glycopeptides
Mass spectrometry
(MS)-based glycoproteomics research requires
highly efficient sample preparation to eliminate interference from
non-glycopeptides and to improve the efficiency of glycopeptide detection.
In this work, a novel MoS<sub>2</sub>/Au-NP (gold nanoparticle)–L-cysteine
nanocomposite was prepared for glycopeptide enrichment. The two-dimensional
(2D) structured MoS<sub>2</sub> nanosheets served as a matrix that
could provide a large surface area for immobilizing hydrophilic groups
(such as L-cysteine) with low steric hindrance between the materials
and the glycopeptides. As a result, the novel nanomaterial possessed
an excellent ability to capture glycopeptides. Compared to commercial
zwitterionic hydrophilic interaction liquid chromatography (ZIC-HILIC)
materials, the novel nanomaterials exhibited excellent enrichment
performance with ultrahigh selectivity and sensitivity (approximately
10 fmol), high binding capacity (120 mg g<sup>–1</sup>), high
enrichment recovery (more than 93%), satisfying batch-to-batch reproducibility,
and good universality for glycopeptide enrichment. In addition, its
outstanding specificity and efficiency for glycopeptide enrichment
was confirmed by the detection of glycopeptides from an human serum
immunoglobulin G (IgG) tryptic digest in quantities as low as a 1:1250
molar ratio of IgG tryptic digest to bovine serum albumin tryptic
digest. The novel nanocomposites were further used for the analysis
of complex samples, and 1920 glycopeptide backbones from 775 glycoproteins
were identified in three replicate analyses of 50 μg of proteins
extracted from HeLa cell exosomes. The resulting highly informative
mass spectra indicated that this multifunctional nanomaterial-based
enrichment method could be used as a promising tool for the in-depth
and comprehensive characterization of glycoproteomes in MS-based glycoproteomics
Two-Dimensional MoS<sub>2</sub>‑Based Zwitterionic Hydrophilic Interaction Liquid Chromatography Material for the Specific Enrichment of Glycopeptides
Mass spectrometry
(MS)-based glycoproteomics research requires
highly efficient sample preparation to eliminate interference from
non-glycopeptides and to improve the efficiency of glycopeptide detection.
In this work, a novel MoS<sub>2</sub>/Au-NP (gold nanoparticle)–L-cysteine
nanocomposite was prepared for glycopeptide enrichment. The two-dimensional
(2D) structured MoS<sub>2</sub> nanosheets served as a matrix that
could provide a large surface area for immobilizing hydrophilic groups
(such as L-cysteine) with low steric hindrance between the materials
and the glycopeptides. As a result, the novel nanomaterial possessed
an excellent ability to capture glycopeptides. Compared to commercial
zwitterionic hydrophilic interaction liquid chromatography (ZIC-HILIC)
materials, the novel nanomaterials exhibited excellent enrichment
performance with ultrahigh selectivity and sensitivity (approximately
10 fmol), high binding capacity (120 mg g<sup>–1</sup>), high
enrichment recovery (more than 93%), satisfying batch-to-batch reproducibility,
and good universality for glycopeptide enrichment. In addition, its
outstanding specificity and efficiency for glycopeptide enrichment
was confirmed by the detection of glycopeptides from an human serum
immunoglobulin G (IgG) tryptic digest in quantities as low as a 1:1250
molar ratio of IgG tryptic digest to bovine serum albumin tryptic
digest. The novel nanocomposites were further used for the analysis
of complex samples, and 1920 glycopeptide backbones from 775 glycoproteins
were identified in three replicate analyses of 50 μg of proteins
extracted from HeLa cell exosomes. The resulting highly informative
mass spectra indicated that this multifunctional nanomaterial-based
enrichment method could be used as a promising tool for the in-depth
and comprehensive characterization of glycoproteomes in MS-based glycoproteomics
Two-Dimensional MoS<sub>2</sub>‑Based Zwitterionic Hydrophilic Interaction Liquid Chromatography Material for the Specific Enrichment of Glycopeptides
Mass spectrometry
(MS)-based glycoproteomics research requires
highly efficient sample preparation to eliminate interference from
non-glycopeptides and to improve the efficiency of glycopeptide detection.
In this work, a novel MoS<sub>2</sub>/Au-NP (gold nanoparticle)–L-cysteine
nanocomposite was prepared for glycopeptide enrichment. The two-dimensional
(2D) structured MoS<sub>2</sub> nanosheets served as a matrix that
could provide a large surface area for immobilizing hydrophilic groups
(such as L-cysteine) with low steric hindrance between the materials
and the glycopeptides. As a result, the novel nanomaterial possessed
an excellent ability to capture glycopeptides. Compared to commercial
zwitterionic hydrophilic interaction liquid chromatography (ZIC-HILIC)
materials, the novel nanomaterials exhibited excellent enrichment
performance with ultrahigh selectivity and sensitivity (approximately
10 fmol), high binding capacity (120 mg g<sup>–1</sup>), high
enrichment recovery (more than 93%), satisfying batch-to-batch reproducibility,
and good universality for glycopeptide enrichment. In addition, its
outstanding specificity and efficiency for glycopeptide enrichment
was confirmed by the detection of glycopeptides from an human serum
immunoglobulin G (IgG) tryptic digest in quantities as low as a 1:1250
molar ratio of IgG tryptic digest to bovine serum albumin tryptic
digest. The novel nanocomposites were further used for the analysis
of complex samples, and 1920 glycopeptide backbones from 775 glycoproteins
were identified in three replicate analyses of 50 μg of proteins
extracted from HeLa cell exosomes. The resulting highly informative
mass spectra indicated that this multifunctional nanomaterial-based
enrichment method could be used as a promising tool for the in-depth
and comprehensive characterization of glycoproteomes in MS-based glycoproteomics
Two-Dimensional MoS<sub>2</sub>‑Based Zwitterionic Hydrophilic Interaction Liquid Chromatography Material for the Specific Enrichment of Glycopeptides
Mass spectrometry
(MS)-based glycoproteomics research requires
highly efficient sample preparation to eliminate interference from
non-glycopeptides and to improve the efficiency of glycopeptide detection.
In this work, a novel MoS<sub>2</sub>/Au-NP (gold nanoparticle)–L-cysteine
nanocomposite was prepared for glycopeptide enrichment. The two-dimensional
(2D) structured MoS<sub>2</sub> nanosheets served as a matrix that
could provide a large surface area for immobilizing hydrophilic groups
(such as L-cysteine) with low steric hindrance between the materials
and the glycopeptides. As a result, the novel nanomaterial possessed
an excellent ability to capture glycopeptides. Compared to commercial
zwitterionic hydrophilic interaction liquid chromatography (ZIC-HILIC)
materials, the novel nanomaterials exhibited excellent enrichment
performance with ultrahigh selectivity and sensitivity (approximately
10 fmol), high binding capacity (120 mg g<sup>–1</sup>), high
enrichment recovery (more than 93%), satisfying batch-to-batch reproducibility,
and good universality for glycopeptide enrichment. In addition, its
outstanding specificity and efficiency for glycopeptide enrichment
was confirmed by the detection of glycopeptides from an human serum
immunoglobulin G (IgG) tryptic digest in quantities as low as a 1:1250
molar ratio of IgG tryptic digest to bovine serum albumin tryptic
digest. The novel nanocomposites were further used for the analysis
of complex samples, and 1920 glycopeptide backbones from 775 glycoproteins
were identified in three replicate analyses of 50 μg of proteins
extracted from HeLa cell exosomes. The resulting highly informative
mass spectra indicated that this multifunctional nanomaterial-based
enrichment method could be used as a promising tool for the in-depth
and comprehensive characterization of glycoproteomes in MS-based glycoproteomics
Two-Dimensional MoS<sub>2</sub>‑Based Zwitterionic Hydrophilic Interaction Liquid Chromatography Material for the Specific Enrichment of Glycopeptides
Mass spectrometry
(MS)-based glycoproteomics research requires
highly efficient sample preparation to eliminate interference from
non-glycopeptides and to improve the efficiency of glycopeptide detection.
In this work, a novel MoS<sub>2</sub>/Au-NP (gold nanoparticle)–L-cysteine
nanocomposite was prepared for glycopeptide enrichment. The two-dimensional
(2D) structured MoS<sub>2</sub> nanosheets served as a matrix that
could provide a large surface area for immobilizing hydrophilic groups
(such as L-cysteine) with low steric hindrance between the materials
and the glycopeptides. As a result, the novel nanomaterial possessed
an excellent ability to capture glycopeptides. Compared to commercial
zwitterionic hydrophilic interaction liquid chromatography (ZIC-HILIC)
materials, the novel nanomaterials exhibited excellent enrichment
performance with ultrahigh selectivity and sensitivity (approximately
10 fmol), high binding capacity (120 mg g<sup>–1</sup>), high
enrichment recovery (more than 93%), satisfying batch-to-batch reproducibility,
and good universality for glycopeptide enrichment. In addition, its
outstanding specificity and efficiency for glycopeptide enrichment
was confirmed by the detection of glycopeptides from an human serum
immunoglobulin G (IgG) tryptic digest in quantities as low as a 1:1250
molar ratio of IgG tryptic digest to bovine serum albumin tryptic
digest. The novel nanocomposites were further used for the analysis
of complex samples, and 1920 glycopeptide backbones from 775 glycoproteins
were identified in three replicate analyses of 50 μg of proteins
extracted from HeLa cell exosomes. The resulting highly informative
mass spectra indicated that this multifunctional nanomaterial-based
enrichment method could be used as a promising tool for the in-depth
and comprehensive characterization of glycoproteomes in MS-based glycoproteomics
Two-Dimensional MoS<sub>2</sub>‑Based Zwitterionic Hydrophilic Interaction Liquid Chromatography Material for the Specific Enrichment of Glycopeptides
Mass spectrometry
(MS)-based glycoproteomics research requires
highly efficient sample preparation to eliminate interference from
non-glycopeptides and to improve the efficiency of glycopeptide detection.
In this work, a novel MoS<sub>2</sub>/Au-NP (gold nanoparticle)–L-cysteine
nanocomposite was prepared for glycopeptide enrichment. The two-dimensional
(2D) structured MoS<sub>2</sub> nanosheets served as a matrix that
could provide a large surface area for immobilizing hydrophilic groups
(such as L-cysteine) with low steric hindrance between the materials
and the glycopeptides. As a result, the novel nanomaterial possessed
an excellent ability to capture glycopeptides. Compared to commercial
zwitterionic hydrophilic interaction liquid chromatography (ZIC-HILIC)
materials, the novel nanomaterials exhibited excellent enrichment
performance with ultrahigh selectivity and sensitivity (approximately
10 fmol), high binding capacity (120 mg g<sup>–1</sup>), high
enrichment recovery (more than 93%), satisfying batch-to-batch reproducibility,
and good universality for glycopeptide enrichment. In addition, its
outstanding specificity and efficiency for glycopeptide enrichment
was confirmed by the detection of glycopeptides from an human serum
immunoglobulin G (IgG) tryptic digest in quantities as low as a 1:1250
molar ratio of IgG tryptic digest to bovine serum albumin tryptic
digest. The novel nanocomposites were further used for the analysis
of complex samples, and 1920 glycopeptide backbones from 775 glycoproteins
were identified in three replicate analyses of 50 μg of proteins
extracted from HeLa cell exosomes. The resulting highly informative
mass spectra indicated that this multifunctional nanomaterial-based
enrichment method could be used as a promising tool for the in-depth
and comprehensive characterization of glycoproteomes in MS-based glycoproteomics
Ultrasmall Pt Nanoclusters as Robust Peroxidase Mimics for Colorimetric Detection of Glucose in Human Serum
In this work, a new type of ultrasmall
Pt nanoclusters (Pt NCs)
was prepared via a facile one-pot approach by using yeast extract
as the reductant and stabilizer. Besides their excellent water solubility,
these yeast extract-stabilized Pt NCs also possess attractive peroxidase
mimicking property. They can efficiently catalyze the oxidation of
3,3,5,5-tetramethylbenzidine (TMB) in the coexistence of hydrogen
peroxide (H<sub>2</sub>O<sub>2</sub>). Catalytic mechanism analysis
suggested that the peroxidase mimicking activity of these Pt NCs might
originate from their characteristic of accelerating electron transfer
between TMB and H<sub>2</sub>O<sub>2</sub>, and their enzymatic kinetics
followed typical Michaelis–Menten theory. On the basis of these
findings, we developed a new highly sensitive colorimetric method
for glucose detection, and the limit of detection was calculated as
low as 0.28 μM (S/N = 3). Further application of the present
system for glucose detection in human serum has been successfully
demonstrated, suggesting its promising utilization as robust peroxidase
mimics in the clinical diagnosis, pharmaceutical, and environmental
chemistry fields