The three bio-markers expression by immunohistochemistry.

<p>(A) High levels of COX-2 expression in hypopharyngeal squamous cell carcinoma: diffuse cytoplasmic staining. (B) Low levels of COX-2 expression in epithelium adjacent to carcinoma. (C) High levels of CD44v6 expression in hypopharyngeal squamous cell carcinoma: cell membrane staining. (D) High levels of CD44v6 expression in epithelium adjacent to carcinoma as well as staining in carcinoma tissues. (E) High levels of CD147 expression in hypopharyngeal squamous cell carcinoma: diffuse cytoplasmic staining. (F) Low level of CD147 expression in epithelium adjacent to carcinoma.</p

The three bio-markers expression in well, moderate and pooly differentiated tumor tissues.

<p>(G) COX-2 expression in well differentiated tumor tissues. (H) COX-2 expression in moderate differentiated tumor tissues. (I) COX-2 expression in poorly differentiated tumor tissues. (J) CD44v6 expression in well differentiated tumor tissues. (K) CD44v6 expression in moderate differentiated tumor tissues. (L) CD44v6 expression in poorly differentiated tumor tissues. (M) CD147 expression in well differentiated tumor tissues. (N) CD147 expression in moderate differentiated tumor tissues. (O) CD147 expression in poorly differentiated tumor tissues.</p

Correlation of clinicopathologic characteristics of the patients with immunohistochemistry results.

*<p><i>P</i><0.001.</p

The three bio-markers expression in carcinoma and adjacent epithelium.

*<p><i>P</i><0.001.</p

The three bio-markers expression in tumor tissues with and without lymph node metastasis.

<p>(P) High levels of COX-2 expression in tumor tissue with lymph node metastasis. (Q) Low levels of COX-2 expression in tumor tissue without lymph node metastasis. (R) High levels of CD44v6 expression in tumor tissue with lymph node metastasis. (S) Low levels of CD44v6 expression in tumor tissue without lymph node metastasis. (T) High levels of CD147 expression in tumor tissue with lymph node metastasis. (U) Low levels of CD147 expression in tumor tissue without lymph node metastasis.</p

Kaplan-Meier curve for the overall survival of the patients with hypopharyngeal carcinoma, according to the expression of P₅₀ of COX-2.

<p>Kaplan-Meier curve for the overall survival of the patients with hypopharyngeal carcinoma, according to the expression of P₅₀ of COX-2.</p

Kaplan-Meier survival analysis by level of the three bio-markers expression.

*<p>log rank <i>P</i><0.05.</p><p>n₁ is the number of total patients of relative percentile;</p><p>n (%) is the number of dead patients of relative percentile (n/n₁*100%).</p

MOESM1 of Knockdown of CLIC4 enhances ATP-induced HN4 cell apoptosis through mitochondrial and endoplasmic reticulum pathways

Additional file 1: Figure S1. Effect of CLIC4 or scrambled siRNA on CLIC4 expression. Effect of CLIC4 or scrambled siRNA on CLIC4 expression. Summarized data showing the expression level of CLIC4. HN4 cells were transfected with CLIC4 or scrambled siRNA, and then were treated with (ATP-Con, ATP-CLIC4) or without (Con, Con-CLIC4) 100 μmol/L ATP for 3 h. β-Tubulin was used as a loading control. Values are shown as the mean ± SE. n = 3. *P < 0.05. vs. the control (Con) group,# P < 0.05 vs. the ATP control (ATP-Con) group