Detection of eight different tospovirus species by a monoclonal antibody against the common epitope of NSs protein

Rabbit antisera against the nucleocapsid protein (NP) have been commonly used for detection of tospoviruses and classification into serogroups or serotypes. Mouse monoclonal antibodies (MAbs) with high specificity to the NPs have also been widely used to identify tospovirus species. Recently, a serogroup-specific MAb against the NSs protein of Watermelon silver mottle virus (WSMoV) was produced by our laboratory to react with five members of WSMoV serogroup, i.e., WSMoV, Capsicum chlorosis virus (CaCV), Calla lily chlorotic spot virus (CCSV), Peanut bud necrosis virus (PBNV) and Watermelon bud necrosis virus (WBNV). The epitope recognized by the NSs MAb was determined and the comparison with the reported sequences of tospoviral NSs proteins revealed that the epitope is highly conserved at the N-terminal region of NSs proteins among members of WSMoV and Iris yellow spot virus (IYSV) serogroups, and Melon yellow spot virus (MYSV) serotype. When the NSs MAb was further used to react with the crude antigens of MYSV serotype, IYSV and Tomato yellow ring virus (TYRV) of IYSV serogroup, strong serological reactions, both in ELISA and western blotting, were observed. Thus, our results indicated that the NSs MAb is a useful and convenient tool for detection of the eight tospovirus species. It is also suggested that these eight Asian-type tospoviruses, i.e., WSMoV, CaCV, CCSV, PBNV, WBNV, MYSV, IYSV and TYRV, may share a common evolutionary ancesto

Helicity conservation and factorization-suppressed charmless B decays

Toward the goal of extracting the weak angle alpha, the decay B^0/B^0-bar to a_0^{+/-}pi^{-/+} was recently measured. The decay B^0 to a_0^+pi^- is not only forbidden in the factorization limit of the tree interaction, but also strongly suppressed for the penguin interaction if short-distance QCD dominates. This makes extraction of alpha very difficult from a^{+/-}\pi^{-/+}. We examine the simlar factorization-suppressed decays, in particular, B^0\to b_1^+pi^-. The prospect of obtaining alpha is even less promising with b_1^{+/-}pi^{-/+}. To probe how well the short-distance dominance works, we emphasize importance of testing helicity conservation in the charmless B decays with spins.Comment: The version to appear in Phys. Rev. D after minor alteration

Helicity conservation in inclusive nonleptonic decay B to VX: Test of long-distance final state interaction

The polarization measurement in the inclusive B decay provides us with a simple test of how much the long-distance final-state interaction takes place as the energy of the observed meson varies in the final state. We give the expectation of the perturbative QCD for the energy dependence of the helicity fractions in a semiquantitative form. Experiment will tell us for which decay processes the perturbative calculation should be applicable.Comment: 15 pages in Revtex with 3 figures embedde

Final-state interaction and s-quark helicity conservation in B -> J/psi K*

The Section of charm quark spin conservation is deleted since it involves more dynamical assumptions than previously stated. A few comments are added in view of new experimental results.Comment: To replace the earlier version of hep-ph/0106354. Minor additions and one deletion with no change in the main argument nor the conclusio

Perturbative QCD analysis of B→ϕK∗B \to \phi K^* decays

We study the first observed charmless $B\to VV$ modes, the $B\to\phi K^*$ decays, in perturbative QCD formalism. The obtained branching ratios $B(B\to\phi K^*)\sim 15 \times 10^{-6}$ are larger than $\sim 9\times 10^{-6}$ from QCD factorization. The comparison of the predicted magnitudes and phases of the different helicity amplitudes, and branching ratios with experimental data can test the power counting rules, the evaluation of annihilation contributions, and the mechanism of dynamical penguin enhancement in perturbative QCD, respectively.Comment: 14 pages, 2 tables, brief disscussion on hard sacle added, version to appear in PR

kTk_T factorization of exclusive processes

We prove $k_T$ factorization theorem in perturbative QCD (PQCD) for exclusive processes by considering $\pi\gamma^*\to \gamma(\pi)$ and $B\to\gamma(\pi) l\bar\nu$. The relevant form factors are expressed as the convolution of hard amplitudes with two-parton meson wave functions in the impact parameter $b$ space, $b$ being conjugate to the parton transverse momenta $k_T$. The point is that on-shell valence partons carry longitudinal momenta initially, and acquire $k_T$ through collinear gluon exchanges. The $b$-dependent two-parton wave functions with an appropriate path for the Wilson links are gauge-invariant. The hard amplitudes, defined as the difference between the parton-level diagrams of on-shell external particles and their collinear approximation, are also gauge-invariant. We compare the predictions for two-body nonleptonic $B$ meson decays derived from $k_T$ factorization (the PQCD approach) and from collinear factorization (the QCD factorization approach).Comment: 11 pages, REVTEX, 5 figure

Study of Bc --> J/psi pi, etac pi decays with perturbative QCD approach

The Bc --> J/psi pi, etac pi decays are studied with the perturbative QCD approach. It is found that form factors and branching ratios are sensitive to the parameters w, v, f_J/psi and f_etac, where w and v are the parameters of the charmonium wave functions for Coulomb potential and harmonic oscillator potential, respectively, f_J/psi and f_etac are the decay constants of the J/psi and etac mesons, respectively. The large branching ratios and the clear signals of the final states make the Bc --> J/psi pi, etac pi decays to be the prospective channels for measurements at the hadron collidersComment: 21 pages, revtex

Restoration of kTk_T factorization for low pTp_T hadron hadroproduction

We discuss the applicability of the $k_T$ factorization theorem to low-$p_T$ hadron production in hadron-hadron collision in a simple toy model, which involves only scalar particles and gluons. It has been shown that the $k_T$ factorization for high-$p_T$ hadron hadroproduction is broken by soft gluons in the Glauber region, which are exchanged among a transverse-momentum-dependent (TMD) parton density and other subprocesses of the collision. We explain that the contour of a loop momentum can be deformed away from the Glauber region at low $p_T$, so the above residual infrared divergence is factorized by means of the standard eikonal approximation. The $k_T$ factorization is then restored in the sense that a TMD parton density maintains its universality. Because the resultant Glauber factor is independent of hadron flavors, experimental constraints on its behavior are possible. The $k_T$ factorization can also be restored for the transverse single-spin asymmetry in hadron-hadron collision at low $p_T$ in a similar way, with the residual infrared divergence being factorized into the same Glauber factor.Comment: 12 pages, 2 figures, version to appear in EPJ

C-reactive protein, sodium azide, and endothelial connexin43 gap junctions

We investigated the effect of C-reactive protein (CRP) and sodium azide (NaN(3)) on endothelial Cx43 gap junctions. Human aortic endothelial cells (HAEC) were treated with (a) detoxified CRP, (b) detoxified dialyzed CRP, (c) detoxified dialyzed CRP plus NaN(3), (d) NaN(3), or (e) dialyzed NaN(3). The concentration of CRP in all preparations was fixed to 25 mu g/ml and that of NaN(3) in the preparations of (c) to (e) was equivalent to that contained in the 25 mu g/ml CRP purchased commercially. The results showed that both the expression of Cx43 protein and gap junctional communication function post-48-h incubation were reduced and inhibited by the detoxified CRP, NaN(3), or detoxified dialyzed CRP plus NaN(3), but not by the detoxified dialyzed CRP or dialyzed NaN(3). Reverse transcription-polymerase chain reaction analysis of cells treated for 72 h also showed a pattern of transcriptional regulation essentially the same as that for the proteins. We concluded that CRP does not have a significant effect on Cx43 gap junctions of HAEC, but NaN(3) inhibited the viability of cells and downregulate their junctions

Nonfactorizable contributions to B→D(∗)MB \to D^{(*)} M decays

While the factorization assumption works well for many two-body nonleptonic $B$ meson decay modes, the recent measurement of $\bar B\to D^{(*)0}M^0$ with $M=\pi$, $\rho$ and $\omega$ shows large deviation from this assumption. We analyze the $B\to D^{(*)}M$ decays in the perturbative QCD approach based on $k_T$ factorization theorem, in which both factorizable and nonfactorizable contributions can be calculated in the same framework. Our predictions for the Bauer-Stech-Wirbel parameters, $|a_2/a_1|= 0.43\pm 0.04$ and $Arg(a_2/a_1)\sim -42^\circ$ and $|a_2/a_1|= 0.47\pm 0.05$ and $Arg(a_2/a_1)\sim -41^\circ$, are consistent with the observed $B\to D\pi$ and $B\to D^*\pi$ branching ratios, respectively. It is found that the large magnitude $|a_2|$ and the large relative phase between $a_2$ and $a_1$ come from color-suppressed nonfactorizable amplitudes. Our predictions for the ${\bar B}^0\to D^{(*)0}\rho^0$, $D^{(*)0}\omega$ branching ratios can be confronted with future experimental data.Comment: 25 pages with Latex, axodraw.sty, 6 figures and 5 tables, Version published in PRD, Added new section 5 and reference