935 research outputs found
Structured illumination microscopy with unknown patterns and a statistical prior
Structured illumination microscopy (SIM) improves resolution by
down-modulating high-frequency information of an object to fit within the
passband of the optical system. Generally, the reconstruction process requires
prior knowledge of the illumination patterns, which implies a well-calibrated
and aberration-free system. Here, we propose a new \textit{algorithmic
self-calibration} strategy for SIM that does not need to know the exact
patterns {\it a priori}, but only their covariance. The algorithm, termed
PE-SIMS, includes a Pattern-Estimation (PE) step requiring the uniformity of
the sum of the illumination patterns and a SIM reconstruction procedure using a
Statistical prior (SIMS). Additionally, we perform a pixel reassignment process
(SIMS-PR) to enhance the reconstruction quality. We achieve 2 better
resolution than a conventional widefield microscope, while remaining
insensitive to aberration-induced pattern distortion and robust against
parameter tuning
2D and 3D structured illumination microscopy with unknown patterns and a statistical prior
Structured illumination microscopy (SIM) is one of the most widely applied super-resolution microscopy techniques in bioimaging. It improves resolution by down-modulating a sample’s high spatial frequency information to fit within the passband of the optical system. Normally, the reconstruction process requires prior knowledge of the illumination patterns. Aberrations from the optical system or from the sample itself will distort the patterns and degrade performance. Here, we propose a new algorithmic self-calibration strategy for both 2D and 3D SIM that does not need to know the exact patterns a priori, but only their covariance. The algorithm, termed PE-SIMS, includes a pattern-estimation (PE) step requiring the uniformity of the sum of the illumination patterns and a SIM reconstruction procedure using a statistical prior (SIMS). We achieve 2x better resolution than a conventional widefield microscope, without needing to know the illumination patterns and while remaining insensitive to aberration-induced pattern distortion.
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Computational illumination for high-speed in vitro Fourier ptychographic microscopy
We demonstrate a new computational illumination technique that achieves large
space-bandwidth-time product, for quantitative phase imaging of unstained live
samples in vitro. Microscope lenses can have either large field of view (FOV)
or high resolution, not both. Fourier ptychographic microscopy (FPM) is a new
computational imaging technique that circumvents this limit by fusing
information from multiple images taken with different illumination angles. The
result is a gigapixel-scale image having both wide FOV and high resolution,
i.e. large space-bandwidth product (SBP). FPM has enormous potential for
revolutionizing microscopy and has already found application in digital
pathology. However, it suffers from long acquisition times (on the order of
minutes), limiting throughput. Faster capture times would not only improve
imaging speed, but also allow studies of live samples, where motion artifacts
degrade results. In contrast to fixed (e.g. pathology) slides, live samples are
continuously evolving at various spatial and temporal scales. Here, we present
a new source coding scheme, along with real-time hardware control, to achieve
0.8 NA resolution across a 4x FOV with sub-second capture times. We propose an
improved algorithm and new initialization scheme, which allow robust phase
reconstruction over long time-lapse experiments. We present the first FPM
results for both growing and confluent in vitro cell cultures, capturing videos
of subcellular dynamical phenomena in popular cell lines undergoing division
and migration. Our method opens up FPM to applications with live samples, for
observing rare events in both space and time
p38α MAPK regulates proliferation and differentiation of osteoclast progenitors and bone remodeling in an aging-dependent manner.
Bone mass is determined by the balance between bone formation, carried out by mesenchymal stem cell-derived osteoblasts, and bone resorption, carried out by monocyte-derived osteoclasts. Here we investigated the potential roles of p38 MAPKs, which are activated by growth factors and cytokines including RANKL and BMPs, in osteoclastogenesis and bone resorption by ablating p38α MAPK in LysM+monocytes. p38α deficiency promoted monocyte proliferation but regulated monocyte osteoclastic differentiation in a cell-density dependent manner, with proliferating p38α-/- cultures showing increased differentiation. While young mutant mice showed minor increase in bone mass, 6-month-old mutant mice developed osteoporosis, associated with an increase in osteoclastogenesis and bone resorption and an increase in the pool of monocytes. Moreover, monocyte-specific p38α ablation resulted in a decrease in bone formation and the number of bone marrow mesenchymal stem/stromal cells, likely due to decreased expression of PDGF-AA and BMP2. The expression of PDGF-AA and BMP2 was positively regulated by the p38 MAPK-Creb axis in osteoclasts, with the promoters of PDGF-AA and BMP2 having Creb binding sites. These findings uncovered the molecular mechanisms by which p38α MAPK regulates osteoclastogenesis and coordinates osteoclastogenesis and osteoblastogenesis
Dynamic Structured Illumination Microscopy with a Neural Space-time Model
Structured illumination microscopy (SIM) reconstructs a super-resolved image
from multiple raw images captured with different illumination patterns; hence,
acquisition speed is limited, making it unsuitable for dynamic scenes. We
propose a new method, Speckle Flow SIM, that uses static patterned illumination
with moving samples and models the sample motion during data capture in order
to reconstruct the dynamic scene with super-resolution. Speckle Flow SIM relies
on sample motion to capture a sequence of raw images. The spatio-temporal
relationship of the dynamic scene is modeled using a neural space-time model
with coordinate-based multi-layer perceptrons (MLPs), and the motion dynamics
and the super-resolved scene are jointly recovered. We validate Speckle Flow
SIM for coherent imaging in simulation and build a simple, inexpensive
experimental setup with off-the-shelf components. We demonstrate that Speckle
Flow SIM can reconstruct a dynamic scene with deformable motion and 1.88x the
diffraction-limited resolution in experiment
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Shape-controlled single-crystal growth of InP at low temperatures down to 220 °C.
III-V compound semiconductors are widely used for electronic and optoelectronic applications. However, interfacing III-Vs with other materials has been fundamentally limited by the high growth temperatures and lattice-match requirements of traditional deposition processes. Recently, we developed the templated liquid-phase (TLP) crystal growth method for enabling direct growth of shape-controlled single-crystal III-Vs on amorphous substrates. Although in theory, the lowest temperature for TLP growth is that of the melting point of the group III metal (e.g., 156.6 °C for indium), previous experiments required a minimum growth temperature of 500 °C, thus being incompatible with many application-specific substrates. Here, we demonstrate low-temperature TLP (LT-TLP) growth of single-crystalline InP patterns at substrate temperatures down to 220 °C by first activating the precursor, thus enabling the direct growth of InP even on low thermal budget substrates such as plastics and indium-tin-oxide (ITO)-coated glass. Importantly, the material exhibits high electron mobilities and good optoelectronic properties as demonstrated by the fabrication of high-performance transistors and light-emitting devices. Furthermore, this work may enable integration of III-Vs with silicon complementary metal-oxide-semiconductor (CMOS) processing for monolithic 3D integrated circuits and/or back-end electronics
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