DD5-ablation sample video

Processed video showing DD5-ablated animal (with segmentation) freely-moving on a bacterial lawn for 15 minutes

PDB ablations_1 feature files and segmented movies

Processed videos of mock- and PDB-ablated animals (with segmentation) freely-moving on a bacterial lawn for 15 minutes, and Matlab files containing data for features extracted from these videos, for replicate 1 of the PDB-ablation experiment

Mock-ablation (PDB) sample video

Processed video showing mock-ablated animal (with segmentation) freely-moving on a bacterial lawn for 15 minutes

DD2 DD5 ablations_2 raw videos (mock)

Raw videos of mock-ablated animals freely-moving on a bacterial lawn for 15 minutes for replicate 2 of the DD2/DD5-ablation experiment

PDB ablations_2 raw videos (mock/PDB)

Raw videos of mock- and PDB-ablated animals freely-moving on a bacterial lawn for 15 minutes, for replicate 2 of the PDB-ablation experiment

DD3 DD4 ablations raw videos (mock/DD3/DD4)

Raw videos of mock-, DD3- and DD4-ablated animals freely-moving on a bacterial lawn for 15 minutes, for the DD3/DD4-ablation experiment

PDB-ablation sample video 1

Processed video showing PDB-ablated animal (with segmentation) freely-moving on a bacterial lawn for 15 minutes

Modes of interaction between connectome layers.

<p>Shown are overrepresented and underrepresented multilink motifs for 3-layer networks consisting of synaptic, gap junction and monoamine (aggregate or individual MA) layers. (A) Multilink motif IDs. These correspond to all possible configurations of links between two neurons allowing for: no connection of a given type (dotted line), directed extrasynaptic monoamine links (Ext, represented as arrows on the top), bidirectional gap junctions (represented as bars in the middle) and synapses (represented as inverted arrowheads on the bottom line). (B-C) Motif <i>z</i>-scores for aggregate monoamines (B), dopamine (C), serotonin (D) or octopamine (E) 3-layer multilink. Plots for tyramine and <i>dop-5/6</i>-containing monoamine multilink are in <a href="http://www.ploscompbiol.org/article/info:doi/10.1371/journal.pcbi.1005283#pcbi.1005283.s003" target="_blank">S3 Fig</a>. Over-represented motifs are represented by red upward-pointing triangles. Under-represented motifs are represented by blue downward-pointing triangles. Non-significant motifs are shown by black squares. Values for randomized null model networks are shown as grey crosses. Asterisks report the significance level using the z-test, with Bonferroni-adjusted p-values: * indicates <i>p</i> ≤ 0.05; ** indicates <i>p</i> ≤ 0.01; *** indicates <i>p</i> ≤ 0.001; **** indicates <i>p</i> ≤ 0.0001. Observed and expected multilink frequencies are in <a href="http://www.ploscompbiol.org/article/info:doi/10.1371/journal.pcbi.1005283#pcbi.1005283.t005" target="_blank">Table 5</a>. Examples of monoamine <i>motif 10</i> are listed in <a href="http://www.ploscompbiol.org/article/info:doi/10.1371/journal.pcbi.1005283#pcbi.1005283.t006" target="_blank">Table 6</a>.</p

Multilayer hub neurons for 3-layer connectomes.

<p>The normalized degree product (<i>k</i>_norm) showing the neurons with the highest degree rank across all of the layers. Rich-club neurons are indicated with ⋆</p

Neuropeptide networks.

<p>(A) Adjacency matrix showing the synaptic (magenta) and neuropeptide (green) networks. (B) Multilayer reducibility dendrograms for individual neuropeptide networks. Layers close on the dendrogram have more overlapping edges and are more reducible. Branching height gives the Jensen-Shannon distance between the layers. Wired and monoamine layers are italicized and indicated with green (MA), blue (gap junction), or magenta (synaptic) boxes. (C) Multilayer expansion of wired, monoamine, and neuropeptide networks. Node positions are the same in all layers.</p