Клинико-морфологические особенности клеточной лейомиомы тела матки

Analysis of clinical-anamnestic and morphological features of a cellular and simple leiomyoma of a uterus is leading. Features of a leiomyoma of a uterus with cellular texture of the site are determined: young age, monthly since 15 years ((20,0 ± 4,6)%), often use ВМК ((38,7 ± 5,6)%), an infrequent combination to infringement of genesial function. Analysis of СD31, Ki-67, and Ag-NORproteins expression in the greater and smaller tumour which have been taken away from one macropreparation was carried out.Проведен анализ клинико-анамнестических и морфологических особенностей клеточной и простой лейомиомы матки. Определены особенности лейомиомы матки с клеточным строением узла: позднее наступление менархе ((20,0 ± 4,6)%), частое использование внутриматочных контрацептивов ((38,7 ± 5,6)%), редкое сочетание с нарушением репродуктивной функции. Проведен сравнительный анализ экспрессии молекулярно-биологических маркеров СD31, Ki-67 и аргирофильных областей ядрышкообразующих районов белков в большей и меньшей опухоли, забранных из одного макропрепарата

ROLE OF GENE POLYMORFISM OF PLASMINOGEN ACTIVATOR INGIBITOR TYPE I AS A RISK FACTOR FOR PREMATURE RUPTURE OF MEMBRANE AT TERM PREGNANCY

The retrospective study was designed to identify association of premature rupture of the fetal membranes (PROM) with carrying polymorphisms in genes encoding folate metabolism and hemostasis in 717 women. More than one hundred potential predictors were analyzed including carriage of thrombogenic genes polymorphisms and genes encoding folate metabolism: FV[Arg506Gln], F II [20210 G/A], MTHFR [Ala222Val], (PAI-I)[-675 5G/4G]. Study revealed that plasminogen activator ingibitor-1 gene polymorphism increases significantly the risk of premature rupture of the fetal membranes in term pregnancy (PROM): heterozygous plasminogen activator ingibitor-1 gene polymorphism is associated with 3.6-fold (95% CI 2.4–5.4; p < 0.001), homozygous plasminogen activator ingibitor-1 gene polymorphism – with 1.7-fold (95% CI 1.1–2.6; p = 0.01) risk rise of PROM

Clinical and morphological features uterin corpus cellular leiomyoma

Analysis of clinical-anamnestic and morphological features of a cellular and simple leiomyoma of a uterus is leading. Features of a leiomyoma of a uterus with cellular texture of the site are determined: young age, monthly since 15 years ((20,0 ± 4,6)%), often use ВМК ((38,7 ± 5,6)%), an infrequent combination to infringement of genesial function. Analysis of СD31, Ki-67, and Ag-NORproteins expression in the greater and smaller tumour which have been taken away from one macropreparation was carried out

Role of disorders related to idiotypic and anti-idiotypic interactions in slowing down the achievement of negative serologic reactions in patients with early onset forms of syphilis after specific therapy

Goal. To study the humoral immunity state in patients with the slowed down achievement of negative serologic reactions after the treatment of early onset forms of syphilis by means of examining the blood serum level of idiotypic and anti-idiotypic antibodies to cardiolipin and p17 Treponema Pallidum antigenic protein. Materials and methods. The study involved 324 patients (39.5% male and 60.5% female) with the slowed down achievement of negative serologic reactions. Primary (idiotypic) antibodies to cardiolipin and p17 protein were obtained using immunochromatographic assays with the help of the Bio Logik LP system. Purified antibodies were concentrated using the ultrafiltration technique with the aid of the XM-100А membrane. To obtain the rabbit antiserum to p17 Treponema Pallidum protein, chinchilla rabbits were immunized using the commercial recombinant p17 protein. To determine anti-cardiolipin idiotypic antibodies in the blood serum, the ELISa method optimized for detecting anti-cardiolipin antibodies was applied. To determine anti-cardiolipin anti-idiotypic antibodies as well as idiotypic and anti-idiotypic antibodies to p17 Treponema Pallidum protein, the standard ELISA method was applied. The following antigens were used to process the pads: F(ab)2 fragments of anti-cardiolipin antibodies (5 μg/mL), recombinant р17 T. pallidum protein (5 μg/mL) and F(ab)2 fragments of antibodies to р17 T. pallidum protein (10 μg/mL). The level of antibodies was assessed based on the absorbancy and expressed in conventional activity units using the K coefficient being the absorbancy of the serum under examination to the mean absorbancy of control serums ratio. The K value exceeding 1.5 conventional units indicated the increased level of antibodies. Results. Patients with the slowed down achievement of negative serologic reactions demonstrated a selective increase in the level of anti-idiotypic antibodies (AIAB) relative to T. pallidum antigens, cardiolipin and p17 protein, vs. first-order antibodies, which points at abnormal mutual regulation between idiotypic antibodies (IAB) and AIAB; the discovered phenomenon lays the immunochemical basis for the formation of a self-sustaining “vicious circle” contributing to the induction of high levels of antibodies to treponema antigens even when the pathogen was destroyed

A study of the level of circulating cytokines in patients with atopic dermatitis

Goal of the study. To study the role of pro-inflammatory and anti-inflammatory cytokines for the course of atopic dermatitis (AD). Materials and methods. The study involved 72 patients aged 25-55 suffering from atopic dermatitis for 2-30 years and with at least two relapses a year. The AD patients were assigned to the following groups depending on the SCORAD (iSc) index: Group 1: light form (iSc 50 points). The control group comprised 23 practically healthy subjects aged 25-35. The level of cytokines (IL-1 β, -4, -6, -8, -10, -12, -13, TNF-α and IFN-γ) in blood serum was determined based on the immune-enzyme assay method. Results. A trend to the increased production of anti-inflammatory cytokines (IL-4, -6, -10, -13) at the exacerbation stage and increased synthesis of pro-inflammatory ones (IFN-γ, TNF-α and IL-12) at the remission stage was observed in patients with atopic dermatitis, which confirms the dysregulation of reciprocal relationships between Th1 and Th2 lymphocyte subpopulations during the disease