Forming Sustainable Communities under Increasing Environmental Constraint and Decreasing Population

Additional file 6: Fig. S5. Contact angle measurement of E. coli knockout strains (A) JM109 (control), (B) △yghW, and (C) △yibT. Three biological replicates were performed

Cell growth of recombinant <i>E. coli</i> JM109 strains in the presence of 4% (v/v) cyclohexane.

<p>The recombinant strains were cultured at 37°C and cyclohexane (CH) was added when OD₆₆₀ reached 0.2 (indicated by the arrow). □, pQE-80L (as the control); ▾, pQE-<i>mmsB</i>; •, pQE- <i>tsf</i>; △, pQE-<i>PSEEN0851</i>.</p

SDS-PAGE analysis of expression of <i>mmsB</i>, <i>PSEEN0851</i>, and <i>tsf</i> in <i>E. coli</i> JM109.

<p>The transformants were grown in LB at 37°C and induced by 1 mM IPTG. Lanes: M, molecular mass marker; 1, pQE-80L (as control); 2, pQE-<i>mmsB</i>; 3, pQE-<i>PSEEN0851</i>; 4, pQE-<i>tsf</i>. Arrowheads indicate the locations of recombinant proteins.</p

Effect of organic solvents on cell growth of <i>P. putida</i> JUCT1 (open bar) and its parent strain JUCS (gray bar).

<p>The strains were initially grown in nutrient medium at 37°C till OD₆₆₀ reached 0.2, and then 60% (v/v) organic solvent was added for further incubation of 5 h.</p

Colony formation of <i>E. coli</i> JM109 strains over-expressing <i>mmsB</i>, <i>tsf</i>, and <i>PSEEN0851</i> on LBGMg agar overlaid with decalin after 24 h incubation at 37°C. JM109 carrying empty pQE-80L was used as the control.

<p>Colony formation of <i>E. coli</i> JM109 strains over-expressing <i>mmsB</i>, <i>tsf</i>, and <i>PSEEN0851</i> on LBGMg agar overlaid with decalin after 24 h incubation at 37°C. JM109 carrying empty pQE-80L was used as the control.</p

MALDI-TOF/TOF analysis of peptides from 5 high-abundance proteins.

a<p>Number of peptides identified for individual protein in MALDI-TOF/TOF analysis.</p>b<p>Number of unique peptides identified for individual protein.</p>c<p>Total amino acid sequence of peptides identified for individual protein.</p>d<p>Percentage of amino acid sequence covered by peptides of individual protein.</p

The oxidation of cyclohexane by <i>E. coli</i> strains.

<p>Incubation condition: 37°C for 8 h with cyclohexane (1%, w/v).</p

Five high-abundance protein spots identified by MALDI-TOF/TOF.

a<p>The expression level of <i>P. putida</i> JUCT1 grown in nutrient medium without solvent.</p>b<p>The expression level of <i>P. putida</i> JUCT1 grown in the presence of 60% (v/v) cyclohexane.</p

MOESM3 of Significantly improved solvent tolerance of Escherichia coli by global transcription machinery engineering

Additional file 3: Figure S3. Recombinant expression of pepB, gapA, sdhB and yfgM in E. coli JM109 and their corresponding knockout strains

MOESM1 of Significantly improved solvent tolerance of Escherichia coli by global transcription machinery engineering

Additional file 1: Figure S1. 2D-PAGE of total proteins of E. coli JM109/pHACM-rpoD WT without solvent