Large-scale analysis of full-length cDNAs from the tomato (Solanum lycopersicum) cultivar Micro-Tom, a reference system for the Solanaceae genomics

<p>Abstract</p> <p>Background</p> <p>The Solanaceae family includes several economically important vegetable crops. The tomato (<it>Solanum lycopersicum</it>) is regarded as a model plant of the Solanaceae family. Recently, a number of tomato resources have been developed in parallel with the ongoing tomato genome sequencing project. In particular, a miniature cultivar, Micro-Tom, is regarded as a model system in tomato genomics, and a number of genomics resources in the Micro-Tom-background, such as ESTs and mutagenized lines, have been established by an international alliance.</p> <p>Results</p> <p>To accelerate the progress in tomato genomics, we developed a collection of fully-sequenced 13,227 Micro-Tom full-length cDNAs. By checking redundant sequences, coding sequences, and chimeric sequences, a set of 11,502 non-redundant full-length cDNAs (nrFLcDNAs) was generated. Analysis of untranslated regions demonstrated that tomato has longer 5'- and 3'-untranslated regions than most other plants but rice. Classification of functions of proteins predicted from the coding sequences demonstrated that nrFLcDNAs covered a broad range of functions. A comparison of nrFLcDNAs with genes of sixteen plants facilitated the identification of tomato genes that are not found in other plants, most of which did not have known protein domains. Mapping of the nrFLcDNAs onto currently available tomato genome sequences facilitated prediction of exon-intron structure. Introns of tomato genes were longer than those of Arabidopsis and rice. According to a comparison of exon sequences between the nrFLcDNAs and the tomato genome sequences, the frequency of nucleotide mismatch in exons between Micro-Tom and the genome-sequencing cultivar (Heinz 1706) was estimated to be 0.061%.</p> <p>Conclusion</p> <p>The collection of Micro-Tom nrFLcDNAs generated in this study will serve as a valuable genomic tool for plant biologists to bridge the gap between basic and applied studies. The nrFLcDNA sequences will help annotation of the tomato whole-genome sequence and aid in tomato functional genomics and molecular breeding. Full-length cDNA sequences and their annotations are provided in the database KaFTom <url>http://www.pgb.kazusa.or.jp/kaftom/</url> via the website of the National Bioresource Project Tomato <url>http://tomato.nbrp.jp</url>.</p

トウガラシ属の種子発芽にみられる変温要求性(農学部門)

トウガラシ属の5種10品種を供試し, 種子発芽における好適温度および変温要求性について検討した。日本の栽培品種は一定温度で高い発芽率を示し, 発芽の好適温度は25℃から35℃の広い範囲に及んだ。しかし, 中央アメリカおよび南アメリカで採取された品種は, 品種ごとに発芽の好適温度は異なり, 20℃以下では発芽が抑制された。供試した10品種のうち3品種は, 一定温度では発芽しなかったが, 変温処理を行なうとただちに発芽した。The present experiment was conducted to study the optimum temperature range and the requirement of alternating temperature for seed germination of 10 cultivars representing 5 species of Capsicum. Japanese cultivars showed high percentages of seed germination at constant temperatures of a wide range from 20℃ to 35℃. Cultivars native to Central or South America showed differing optimum temperatures for seed germination, which was inhibited at temperatures below 20℃. Seeds of 3 wild species of Capsicum did not germinate at constant temperatures, but immediately did when subjected to alternating temperatures

Study on high efficiency milling of steam turbine blade

In response to global environmental issues, higher efficiency and improved operational reliability are increasingly being requested for steam turbines, essential equipment for thermal power generation. Its material is stainless steel, which is difficult to cut because of high work-hardening trend and high resistance to heat. As its complex shape and high accuracy requirement, conventional methods such as using a ball end mill have obstacles of achieve a high accuracy coexist with high efficiency. The main objectives of this study are to propose a new method called Linear Interpolation Milling(LIM) using a taper end mill and verify its validity both in theory and practice.1st International Conference on Renewable Energy Research and Applications, ICRERA 2012; Nagasaki; Japan; 11 November 2012 ～ 14 November 201