53 research outputs found
Selective Fluorescence Detection of Cysteine over Homocysteine and Glutathione Based on a Cysteine-Triggered Dual Michael Addition/Retro-aza-aldol Cascade Reaction
In this work, a cysteine (Cys)-triggered
dual Michael addition/retro-aza-aldol
cascade reaction has been exploited and utilized to construct a fluorescent
probe for Cys for the first time. The resulting fluorescent probe
8-alkynylBodipy <b>1</b> contains an activated alkynyl unit
as Michael receptor and a Bodipy dye as fluorescence reporter and
can highly selectively detect Cys over homocysteine (Hcy)/glutathione
(GSH) as well as other amino acids with a significant fluorescence
off–on response (∼4500-fold) and an ultralow detection
limit (0.38 nM). The high selectivity of <b>1</b> for Cys could
be attributed to a kinetically favored five-membered cyclic intermediate
produced by the dual Michael addition of Cys with the activated alkynyl
unit of <b>1</b>. The big fluorescence off–on response
is due to the subsequent retro-aza-aldol reaction of the five-membered
cyclic intermediate that results in the release of a highly fluorescent
8-methylBodipy dye <b>2</b>. The probe has been successfully
used to detect and image Cys in serum and cells, respectively
Funnel plot with 95% confidence intervals (CI) to assess for evidence of publication bias.
<p>Funnel plot with 95% confidence intervals (CI) to assess for evidence of publication bias.</p
Characteristics of included studies-continued.
<p>Statin = statin-treated group (high-dose);Control = control group (low-dose or non-statin);CAG = coronary angiography;PCI = percutaneous coronary intervention;CrCl = creatinine clearance;Scr = serum creatinine;CRP = C-reactive protein;eGFR = estimated glomerular filtration rate;NAC = N-acetylcysteine;NS = 0.9% sodium chloride; NS = not specified or available.</p
Characteristics of included studies.
<p>Statin = statin-treated group(high-dose);Control = control group(low-dose or non-statin);CAG = coronary angiography;PCI = percutaneous coronary intervention;CrCl = creatinine clearance;Scr = serum creatinine;eGFR = estimated glomerular filtration rate;NAC = N-acetylcysteine;NS = 0.9% sodium chloride.</p
Forest plot of risk ratios and 95% confidence intervals (CI) for the incidence of CIN among patients assigned to short-term high-dose statin treatment versus low-dose or non-statin.
<p>Forest plot of risk ratios and 95% confidence intervals (CI) for the incidence of CIN among patients assigned to short-term high-dose statin treatment versus low-dose or non-statin.</p
Forest plot of risk ratios and 95% confidence intervals (CI) for the incidence of CIN among patients assigned to statin therapy versus control according to renal function.
<p>Forest plot of risk ratios and 95% confidence intervals (CI) for the incidence of CIN among patients assigned to statin therapy versus control according to renal function.</p
Forest plot of risk ratios and 95% confidence intervals (CI) for the incidence of CIN among patients assigned to statin therapy versus control according to Jadad score.
<p>Forest plot of risk ratios and 95% confidence intervals (CI) for the incidence of CIN among patients assigned to statin therapy versus control according to Jadad score.</p
Forest plot of risk ratios and 95% confidence intervals (CI) for the incidence of CIN among patients assigned to statin therapy versus control with NAC using or not.
<p>Forest plot of risk ratios and 95% confidence intervals (CI) for the incidence of CIN among patients assigned to statin therapy versus control with NAC using or not.</p
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