Extended Thermal X-ray Emission from the Spiral-Dominant Group of Galaxies HCG 57

We observed a group of galaxies, HCG 57, with ASCA. Regardless that their member galaxies are dominated by spiral galaxies, we detected extended thermal X-ray emission that is attributed to hot gas with a temperature of $1.04\pm0.10$ keV. This is the second clear detection of thermal X-ray emission from a spiral-dominant group of galaxies after HCG 92. The luminosity of the thermal emission is about $5\times10^{41}$ erg s$^{-1}$ in the 0.5--10 keV band, which is higher than that of HCG 92, but relatively less luminous among groups of galaxies. The X-ray emission is extended over several member galaxies, and is thus associated with the group rather than an individual galaxy. The metal abundance cannot be well constrained with a lower limit of 0.08 solar. The gas-to-stellar mass ratio is $\sim0.3$. Although this is relatively low among groups, the hot gas is also a significant component even in the spiral-dominant group. We suggest that the X-ray faintness of spiral-dominant groups is due to the low surface brightness and somewhat low gas mass, at least in the case of HCG 57.Comment: 12 pages, 4 figures, to appear in PASJ 54 No.

Chandra X-Ray Spectral Analysis of Cooling Flow Clusters, 2A 0335+096 and Abell 2199

We report on a spatially resolved analysis of Chandra X-ray data on a nearby typical cooling flow cluster of galaxies 2A 0335+096, together with A 2199 for a comparison. As recently found in the cores of other clusters, the temperature around the central part of 2A 0335+096 is 1.3--1.5 keV, which is higher than that inferred from the cooling flow picture. Furthermore, the absorption column density is almost constant against the radius in 2A 0335+096; there is no evidence of excess absorption up to 200--250 kpc. This indicates that no significant amount of cold material, which has cooled down, is present. These properties are similar to those of A 2199. Since the cooling time in the central part is much shorter than the age of the clusters, a heating mechanism, which weakens the effect of radiative cooling, is expected to be present in the central part of both clusters of galaxies. Both 2A 0335+096 and A 2199 have radio jets associated with their cD galaxy. We discuss the possibility of heating processes caused by these radio jets by considering the thermal conduction and the sound velocity together with the observed disturbance of the ICM temperature and density. We conclude that the observed radio jets can produce local heating and/or cooling, but do not sufficiently reduce the overall radiative cooling. This implies that much more violent jets, whose emission has now decayed, heated up the cooling gas $>10^9$ years ago.Comment: 13 pages, 7 figures, to appear in PASJ 55 No.

Formation of Nanometer-Thick Water Layer at High Humidity on Dynamic Crystalline Material Composed of Multi-Interactive Molecules

Crystalline powders self-assembled from interactive discrete molecules reversibly transformed from a porous structure to a 2D one with a nanometer-thick H2O layer by hydration/dehydration. Multi-point weak intermolecular interactions contributed to maintenance of each phase. This structure transformation induced a humidity-dependent ion conductivity change from insulator to 3.4 x 10(-3) S cm(-1).open1122sciescopu

Hard X-ray Properties of the Merging Cluster Abell 3667 as Observed with Suzaku

Wide-band Suzaku data on the merging cluster Abell 3667 were examined for hard X-ray emission in excess to the known thermal component. Suzaku detected X-ray signals in the wide energy band from 0.5 to 40 keV. The hard X-ray (> 10 keV) flux observed by the HXD around the cluster center cannot be explained by a simple extension of the thermal emission with average temperature of ~7 keV. The emission is most likely an emission from a very hot (kT > 13.2 keV) thermal component around the cluster center, produced via a strong heating process in the merger. In the north-west radio relic, no signature of non-thermal emission was observed. Using the HXD, the overall upper-limit flux within a 34'x34' field-of-view around the relic is derived to be 5.3e-12 erg s-1 cm-2 in the 10-40 keV band, after subtracting the ICM contribution estimated using the XIS or the XMM-Newton spectra. Directly on the relic region, the upper limit is further tightened by the XIS data to be less than 7.3e-13 erg s-1 cm-2, when converted into the 10--40 keV band. The latter value suggest that the average magnetic field within the relic is higher than 1.6 uG. The non-thermal pressure due to magnetic fields and relativistic electrons may be as large as ~20% of the thermal pressure in the region.Comment: 18 pages, 13 figures, to be appeared in PASJ 200

Development of high-throughput quantitative analytical method for l-cysteine-containing dipeptides by LC–MS/MS toward its fermentative production

l-Cysteine (Cys) is metabolically fundamental sulfur compound and important components in various cellular fac-tors. Interestingly, free-form Cys itself as a simple monomeric amino acid was recently shown to function in a novel antioxidative system (cysteine/cystine shuttle system) in Escherichia coli. However, as for Cys-containing dipeptides, the biological functions, effects, and even contents have still remained largely elusive. The potential functions should be a part of cellular redox system and important in basic and applied biology. For its progress, establishment of reli-able quantitation method is the first. However, such accurate analysis is unexpectedly difficult even in Cys, because thiol compounds convert through disulfide-exchange and air oxidation during sample preparation. Addressing this problem, in this study, thiol molecules like Cys-containing dipeptides were derivatized by using monobromobimane (thiol-specific alkylating reagent) and detected as S-bimanyl derivatives by liquid chromatography coupled to tandem mass spectrometry (LC–MS/MS). Sample separation was processed with a C18 column (2.1 mm × 150 mm, 1.7 μm) and with water-acetonitrile gradient mobile phase containing 0.1% (v/v) formic acid at flow rate of 0.25 ml/min. The mass spectrometer was operated in the multiple reaction monitoring in positive/negative mode with electrospray ionization. The derivatization could indeed avoid the unfavorable reactions, namely, developed the method reflecting their correct contents on sampling. Furthermore, the method was successfully applied to monitoring Cys-containing dipeptides in E. coli Cys producer overexpressing bacD gene. This is the first report of the quantitative analysis of Cys-containing dipeptides, which should be useful for further study of fermentative production of Cys-containing dipeptides

A promoter haplotype of the interleukin-18 gene is associated with juvenile idiopathic arthritis in the Japanese population

Recently, we reported that genetic polymorphisms within the human IL18 gene were associated with disease susceptibility to adult-onset Still's disease (AOSD), which is characterized by extraordinarily high serum levels of IL-18. Because high serum IL-18 induction has also been observed in the systemic type of juvenile idiopathic arthritis (JIA), we investigated whether similar genetic skewing is present in this disease. Three haplotypes, S01, S02, and S03, composed of 13 genetic polymorphisms covering two distinct promoter regions, were determined for 33 JIA patients, including 17 with systemic JIA, 10 with polyarthritis, and 6 with oligoarthritis. Haplotypes were also analyzed for 28 AOSD patients, 164 rheumatoid arthritis (RA) patients, 102 patients with collagen diseases, and 173 healthy control subjects. The frequency of individuals carrying a diplotype configuration (a combination of two haplotypes) of S01/S01 was significantly higher in the JIA patients, including all subgroups, than in the healthy controls (P = 0.0045, Fischer exact probability test; odds ratio (OR) = 3.55, 95% confidence interval (CI) = 1.55–8.14). In patients with systemic JIA, its frequency did not differ statistically from that of normal controls. Nevertheless, it is possible that haplotype S01 is associated with the phenotype of high IL-18 production in systemic JIA because the patients carrying S01/S01 showed significantly higher serum IL-18 levels compared with patients with other diplotype configurations (P = 0.017, Mann-Whitney U test). We confirmed that the frequency of the diplotype configuration of S01/S01 was significantly higher in AOSD patients than in healthy control subjects (P = 0.011, OR = 3.45, 95% CI = 1.42–8.36). Furthermore, the RA patients were also more predisposed to have S01/S01 (P = 0.018, OR = 2.00, 95% CI = 1.14–3.50) than the healthy control subjects, whereas the patients with collagen diseases did not. In summary, the diplotype configuration of S01/S01 was associated with susceptibility to JIA as well as AOSD and RA, and linked to significantly higher IL-18 production in systemic JIA. Possession of the diplotype configuration of S01/S01 would be one of the genetic risk factors for susceptibility to arthritis in the Japanese population