Feasibility of cryopreserved tracheal xenotransplants with the use of short-course immunosuppression

AbstractObjective: We evaluated the feasibility of discordant xenotransplantation of the cryopreserved trachea with intermittent immunosuppression to help solve the shortage of donor tracheas. Methods: Two experiments were performed with heterotopic transplantation models in 14 guinea pigs and 85 rats. So that the minimal dose of FK506 for viable fresh xenografts could be determined, FK506 was given in escalating doses (0, 1.5, 2.5, and 3.5 mg/kg) for recipient animals after xenogeneic transplantation. With the goal of obtaining a long-term survival of the xenografts, the effect of cryopreservation on xenografts was assessed and thereafter different cycles of immunosuppression every third week were evaluated in fresh or cryopreserved xenografts in the second experiment. Results: An FK506 dosage of more than 2.5 mg/kg per day was much more effective than smaller dosages, as demonstrated by morphologic assessment. A higher dosage of FK506 potentially delayed the rejection of xenografts and can thus maintain tracheal xenograft viability for less than 4 weeks in rat recipients. In experiment 2, the cryopreserved xenografts showed less histologic viability than fresh xenografts but greater patency of the lumen. The patency of cryopreserved xenografts was favorably maintained for a longer period than that of fresh xenografts with either the same number or more cycles of immunosuppression. Conclusions: We conclude that the synergistic effect of cryopreservation and adequate intermittent immunosuppression may enable tracheal xenografts to remain viable over longer periods. (J Thorac Cardiovasc Surg 2001;121:241-8

Significance of Smoking as a Postoperative Prognostic Factor in Patients with Non-small Cell Lung Cancer

IntroductionIn this study, we investigated the influence of smoking on the postoperative prognosis in patients with non-small cell lung cancer.MethodsThe subjects consisted of 770 patients who underwent a resection of lung cancer in our department between 1994 and 2005. We compared the clinico-pathological findings between the smoking and never-smoking groups. The pack-year index (PYI) was used as a smoking index.ResultsThe smoking group consisted of 569 patients (74%), and the never-smoking group consisted of 201 patients (26%). The smokers were composed of 492 men and 77 women. Among the adenocarcinoma patients, there were 293 (61%) smokers and 185 (39%) never-smokers. The patients with squamous cell carcinoma included 204 (95%) smokers and 10 (5%) never-smokers. The proportion of patients with stage IA disease was significantly higher in the never-smokers than that of the smokers. The 5-year survival rate after surgery was 66% in the never-smoking group; however, the rates were 56% in patients with a PYI more than or equal to 20, and 55% in those with PYI more than 20. Seventy-nine (13.9%) patients in the smoking group and seven (3.5%) patients in the never-smoking group died of other diseases, with a significant difference (p < 0.01). Of these patients, 44 (56%) and 13 (16%) in the smoking group died of respiratory and cardiovascular disorders, respectively. In our series, excluding those who died of other diseases, there were no significant differences in the postoperative prognosis.ConclusionsIn the smoking group, the prognosis was poorer than that in the never-smoking group. The higher proportion of early stage disease (stage IA) and female gender were major causes of the better prognosis of the never-smokers. Nevertheless, the high pulmonary/cardiovascular complication-related mortality was another cause of the poor prognosis of the smokers with lung cancer

SALON: Simplified Sensing System for Activity of Daily Living in Ordinary Home

As aging populations continue to grow, primarily in developed countries, there are increasing demands for the system that monitors the activities of elderly people while continuing to allow them to pursue their individual, healthy, and independent lifestyles. Therefore, it is required to develop the activity of daily living (ADL) sensing systems that are based on high-performance sensors and information technologies. However, most of the systems that have been proposed to date have only been investigated and/or evaluated in experimental environments. When considering the spread of such systems to typical homes inhabited by elderly people, it is clear that such sensing systems will need to meet the following five requirements: (1) be inexpensive; (2) provide robustness; (3) protect privacy; (4) be maintenance-free; and, (5) work with a simple user interface. In this paper, we propose a novel senior-friendly ADL sensing system that can fulfill these requirements. More specifically, we achieve an easy collection of ADL data from elderly people while using a proposed system that consists of a small number of inexpensive energy harvesting sensors and simple annotation buttons, without the need for privacy-invasive cameras or microphones. In order to evaluate the practicality of our proposed system, we installed it in ten typical homes with elderly residents and collected the ADL data over a two-month period. We then visualized the collected data and performed activity recognition using a long short-term memory (LSTM) model. From the collected results, we confirmed that our proposed system, which is inexpensive and non-invasive, can correctly collect resident ADL data and could recognize activities from the collected data with a high recall rate of 72.3% on average. This result shows a high potential of our proposed system for application to services for elderly people