El Diario de Pontevedra : periódico liberal: Ano XXIV Número 6898 - 1907 abril 17

Main clinical and morphological characteristics of human placentae from the alcohol-exposed group. (DOC 131 kb

El Diario de Pontevedra : periódico liberal: Ano XXVIII Número 8256 - 1911 novembro 18

Histomorphometric characterization of the effects of in utero alcohol exposure on human placentae from WG20 to WG25. (a, b) Immunohistochemistry performed against CD31 and toluidine blue counterstaining visualizing microvessels (brown) present in placental villi (blue) from control and alcohol-exposed groups collected at gestational ages ranging from [20–25 WG]. (c) Percentage of villi classified by sizes in placentae from control and alcohol-exposed groups collected at gestational ages ranging from [20–25 WG].*p < 0.05 vs the control group using the unpaired t test (d) Repartition of vessels per size of villi in placentae from control and alcohol-exposed groups collected at gestational ages ranging from [20–25 WG]. (e) Luminal vascular area per size of villi in placentae from control and alcohol-exposed groups collected at gestational ages ranging from [20–25 WG]. (TIFF 19794 kb

Additional file 5: Table S5. of PLGF, a placental marker of fetal brain defects after in utero alcohol exposure

Main clinical and morphological characteristics of human placentae from the alcohol-exposed group. (DOC 131 kb

Additional file 7: Table S7. of PLGF, a placental marker of fetal brain defects after in utero alcohol exposure

Statistical analysis. (DOCX 23 kb

Effect of Substance P on the cytotoxic activity of <i>Bacillus cereus</i>.

<p>(<b>A</b>) Dose response curve of the effect of Substance P (SP) and of the reversed sequence peptide (SPrev). (<b>B</b>) Time course response of the bacteria to SP and SPrev (10⁻⁶ M). ( = p<0.001).</p

Effect of Substance P treated <i>Bacillus cereus</i> on caspase 1 induction and on the morphology of HaCaT cells.

<p>(<b>A</b>) Cells exposed to SP treated bacteria showed an increase of caspase 1 production ( = <i>p<0.05</i>). (<b>B</b>) After a 30 min infection with control bacteria the morphology of HaCaT cells remained unchanged but when the cells were exposed to SP treated <i>B. cereus</i> we observed a complete disorganization of the actin cytoskeleton (□) and the formation of multiple cytoplasmic vacuoles (γ). Bar = 20 µm.</p

Bidimensional electrophoresis analysis of secreted proteins by <i>Bacillus cereus</i> after a 13 h exposure to Substance P reverse (Control) or Substance P (SP) (10⁻⁶ M).

<p>(<b>A</b>) Twelve spots were modified after exposure of the bacteria to SP. (<b>B</b>) Two proteins (1 and 2) were over produced whereas the expression of 10 others was down regulated. Identified proteins are presented in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0078773#pone-0078773-t001" target="_blank">Table 1</a>. Results are representative of three independent experiments.</p

SDS page analysis of secreted Bacillus cereus proteins after 1 or 13(SP) or Substance P reverse (SPrev) (10⁻⁶ M).

<p>Bacteria exposed for 1 h to SP showed an increase in the production of the collagenase (ColT) and of superoxide dismutase (SOD). Bacteria treated for 13 h with SP presented in contrast an increase in the S-layer crystal protein (SL). Results are representative of three independent experiments.</p

TEM observation of control (SPrev) and Substance P (SP) treated <i>Bacillus cereus</i>.

<p>Whereas control bacteria presented a continuous intact surface (<b>A</b>), bacteria exposed to SP showed a detachment of the S-layer (γ) (<b>B</b>). Long amorphous structures were observed in the vicinity of bacterial, suggesting a desquamation of the S-layer (<b>C, D</b>).</p

MALDI-TOF/TOF identification of the proteins in Figure 4 which expression was modified by Substance P in <i>Bacillus cereus</i>.

<p>MALDI-TOF/TOF identification of the proteins in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0078773#pone-0078773-g004" target="_blank">Figure 4</a> which expression was modified by Substance P in <i>Bacillus cereus</i>.</p