Yield of protein recovery and degree of hydrolysis associated protein hydrolysates from Bighead Carp (Aristichthys nobilis) by using enzymes

The present study compared the properties of Bighead carp (Aristichthys nobilis) visceral, viscera use of commercial enzymes (Alcalase, Papain, and Protamex), recovery protein and degree of hydrolysis in the various temperatures and times of hydrolysis process. According to results, increase in time and temperature in hydrolysis process, led to increase in both recovery protein and degree of hydrolysis. Maximum of these parameters were observed at 55°C and 60min. minimum of parameters were observed at 35°C and 15min. In addition, among enzymes being investigated, Alcalase had high rate of recovery protein and degree of hydrolysis in equal temperature and times different from others enzymes (Papain and Protamex). Maximum mean of recovery protein for Alcalase was %51.38±2.39 in 55°C and 60 min of hydrolysis process. Also, maximum degree of hydrolysis for Alcalaqse was more than 20% at 55°C and 60 min of hydrolysis process. In conclusion, use of Alcalase enzyme induces product protein hydrolysates from Bighead Carp (Aristichthys nobilis) viscera that have suitable quality than others enzymes (Papain, and Protamex)

Barriers to Family Caregivers’ Coping With Patients With Severe Mental Illness in Iran

The broad spectrum of problems caused by caring for a patient with mental illness imposes a high burden on family caregivers. This can affect how they cope with their mentally ill family members. Identifying caregivers’ experiences of barriers to coping is necessary to develop a program to help them overcome these challenges. This qualitative content analysis study explored barriers impeding family caregivers’ ability to cope with their relatives diagnosed with severe mental illness (defined here as schizophrenia, schizoaffective disorders, and bipolar affective disorders). Sixteen family caregivers were recruited using purposive sampling and interviewed using a semi-structured in-depth interview method. Data were analyzed by a conventional content analytic approach. Findings consisted of four major categories: the patient’s isolation from everyday life, incomplete recovery, lack of support by the mental health care system, and stigmatization. Findings highlight the necessity of providing support for caregivers by the mental health care delivery service system.The study was supported by Grant TBZMED·REC.5825 from the deputy of research in Tabriz University of Medical Sciences

Antimicrobial resistance of nosocomial strain of Acinetobacter baumannii in Children's Medical Center of Tehran: a 6-year prospective study

There are increasing reports of emergence of multiple drug resistant (MDR) Acinetobacter spp in the world; however there are a few reports in our country. 145 A. baumannii isolates from distinct wards and Children's Medical Center (CMC) in Tehran were studied in order to find the profile of antibiotic resistance among them. 40.6 (59/145) of A. baumannii isolates were identified as MDR. Overall susceptibility rates to cotrimoxazole, chloramphenicole and ciprofloxacin were 23.4, 16.9 and 20.1, respectively. Frequency susceptibility rates to amikacin, kanamycin, gentamycin and tobramycin decreased gradually from 81.2, 50, 50 and 62.5 in 2002 to 25, 15.6, 28.1 and 25 in 2007 respectively. Overall susceptibility rates to cephalosporines cephalotin, ceftazidime, cefteriaxon, ceftizoxime and cefixime were 9.3, 14.7, 16.2, 15.9 and 18, respectively. Susceptibility to carbapenems was assessed only in 2007. The susceptibility rates of Imipenem and meropenem were shown to be 50 and 46.8, respectively. Our data indicates that MDR A. baumannii strains are spreading and carbapenem resistance is becoming more common in Iran. Our findings also highlight the importance of clinicians' access to updated susceptibility data regarding A. baumannii in developing countries such as Iran