Genomic determinants of nitric oxide biosynthesis in Lactobacillus plantarum: Potential opportunities and reality

As the genomic determinants of nitric oxide (NO) biosynthesis were identified in mammals and microorganisms, it became possible to systematically analyze the genetic conditioning of the process in clinically significant lactobacilli. A computer screening of the genetic determinants of synthetic pathways leading to NO production was performed to verify the NO-synthase origin of NO in Lactobacillus plantarum. Experimental evidence for enzymatic NO generation from L-arginine, rather than nitrite, was obtained by EPR spectroscopy. It was shown with the example of L. plantarum NO synthase that the observed functional activity of proteins is due to a complex transformation of the genetic program into a real catalytic function under certain conditions. © 2007 Pleiades Publishing, Inc

Involvement of Iron in Biofilm Formation by Lactobacillus rhamnosus

© 2016, Springer Science+Business Media New York.We investigated the effect of high iron availability on growth and biofilm formation of Lactobacillus rhamnosus strain BB. Biofilm development by L. rhamnosus BB was significantly increased by adding iron into the MRS medium, while bacterial growth was not affected. Iron chelator EDTA had no effect on L. rhamnosus BB growth and biofilm formation, but prevented the stimulation of biofilm development by iron. Our results are the first evidence of iron involvement in biofilm formation by lactobacilli—bacteria that were considered to be iron independent with poor biofilm formation capacity

Modulation of nitric oxide (NO) biosynthesis in lactobacilli

We characterized effects of nitric oxide synthase (NOS) substrate L-arginine and classical inhibitors of mammalian NOS on nitric oxide (NO) biosynthesis in probiotic bacteria Lactobacillus plantarum 8P-A3. NO-synthase origin of nitric oxide detected by fluorescent NO indicator 1,2-diaminoanthraquinone (DAA) was confirmed by induction of NO production by exogenous L-arginine. None of the used inhibitors of three isoforms of mammalian NOSs (L-NAME, L-NIL, nNOS inhibitor I) showed significant inhibitory effect of lactobacillar NO-synthase activity. © 2011 Allerton Press, Inc

Alternative pathways of nitric oxide formation in Lactobacilli: Evidence for nitric oxide synthase activity by EPR

The study of the ability of Lactobacillus plantarum 8P-A3 to synthesize nitric oxide (NO) showed that this strain lacks nitrite reductase. However, analysis by the EPR method revealed the presence of nitric oxide synthase activity in this strain. Like mammalian nitric oxide synthase, lactobacillar NO synthase is involved in the formation of nitric oxide from L-arginine. L. plantarum 8P-A3 does not produce NO in the denitrification process. The regulatory role of NO in symbiotic bacteria is emphasixed. © Nauka/Interperiodica 2006

Secretome of Intestinal bacilli: A natural guard against pathologies

© 2017 Ilinskaya, Ulyanova, Yarullina and Gataullin. Current studies of human gut microbiome usually do not consider the special functional role of transient microbiota, although some of its members remain in the host for a long time and produce broad spectrum of biologically active substances. Getting into the gastrointestinal tract (GIT) with food, water and probiotic preparations, two representatives of Bacilli class, genera Bacillus and Lactobacillus, colonize epithelium blurring the boundaries between resident and transient microbiota. Despite their minor proportion in the microbiome composition, these bacteria can significantly affect both the intestinal microbiota and the entire body thanks to a wide range of secreted compounds. Recently, insufficiency and limitations of pure genome-based analysis of gut microbiota became known. Thus, the need for intense functional studies is evident. This review aims to characterize the Bacillus and Lactobacillus in GIT, as well as the functional roles of the components released by these members of microbial intestinal community. Complex of their secreted compounds is referred by us as the "bacillary secretome." The composition of the bacillary secretome, its biological effects in GIT and role in counteraction to infectious diseases and oncological pathologies in human organism is the subject of the review

Meat quality of quail fed diets enriched with probiotic lactobacilli

The article describes the effect of probiotic strains Lacticaseibacillus rhamnosus AG16, Limosilactobacillus fermentum HFD1, and Lactiplantibacillus plantarum LS-4.4 on the meat quality of quail. The strains were grown on a whey-based nutrient medium, and in the form of suspension were introduced into the diet of birds for two months. The use of these feed additives, especially L. fermentum HFD1, resulted in a decrease in the pH of the quail breast meat. All tested strains induced a decrease in water holding capacity and cooking loss of quail breast meat compared to the control variant. The meat of quail fed with probiotic lactobacilli was less stiff, that resulted in less chewing effort in the raw meat and less cutting effort in the cooked meat

Probiotics for plants: NO-producing lactobacilli protect plants from drought

After the inoculation of wheat roots with a suspension of the bacterium Lactobacillus plantarum, reduction or decrease of oxidative stress detected by the accumulation of H2O2 and MDA was found in leaves. Activation of catalase and increased integral antioxidant capacity in seedlings treated with NO-producing lactobacilli were detected during the determination of the contribution of bacterial NO to the plant stress reaction. Thus, for the first time, we have demonstrated that lactobacilli affect plant adaptive responses to stress by the involvement of nitric oxide. © 2014 Pleiades Publishing, Inc

Role of Nitric Oxide Produced by Lactobacilli in Relaxation of Intestinal Smooth Muscles

© 2016, Springer Science+Business Media New York.Application of NO-producing lactobacilli to a rat jejunum segment induced muscle relaxation that was potentiated after activation of bacterial NO production with NO synthase substrate L-arginine. Similar changes in the intestinal contractile activity were observed in response to exogenous NO formed by sodium nitroprusside. These results indicated the involvement of NO synthesized by probiotic lactobacilli in the regulation of the intestinal motor function

Role of Nitric Oxide Produced by Lactobacilli in Relaxation of Intestinal Smooth Muscles

© 2016 Springer Science+Business Media New York Application of NO-producing lactobacilli to a rat jejunum segment induced muscle relaxation that was potentiated after activation of bacterial NO production with NO synthase substrate L-arginine. Similar changes in the intestinal contractile activity were observed in response to exogenous NO formed by sodium nitroprusside. These results indicated the involvement of NO synthesized by probiotic lactobacilli in the regulation of the intestinal motor function

Role of Nitric Oxide Produced by Lactobacilli in Relaxation of Intestinal Smooth Muscles

© 2016, Springer Science+Business Media New York.Application of NO-producing lactobacilli to a rat jejunum segment induced muscle relaxation that was potentiated after activation of bacterial NO production with NO synthase substrate L-arginine. Similar changes in the intestinal contractile activity were observed in response to exogenous NO formed by sodium nitroprusside. These results indicated the involvement of NO synthesized by probiotic lactobacilli in the regulation of the intestinal motor function