Case report: Sarcomatoid urothelial carcinoma of the renal pelvis masquerading as a renal abscess

Sarcomatoid urothelial carcinoma (SUC), a rare tumor of the urinary tract epithelium, exhibits a high degree of malignancy and therefore a poor prognosis. Due to the absence of specific clinical presentations and imaging findings, SUC of the renal pelvis masquerades as a renal abscess is frequently under-recognized or misdiagnosed as benign inflammatory disease, resulting in delayed or erroneous treatment. Here, we report a patient with SUC of the renal pelvis who presented with a renal abscess. Repeated anti-inflammatory treatment was ineffective. Unexpectedly, cancerous cells were detected in subsequent exfoliative cytology of nephrostomy drainage fluid. In accordance with this, radical surgery and postoperative chemotherapy were conducted. Fortunately, neither recurrence nor metastasis occurred during a one-year follow-up

Genome-wide analysis of FRF gene family and functional identification of HvFRF9 under drought stress in barley

FHY3 and its homologous protein FAR1 are the founding members of FRS family. They exhibited diverse and powerful physiological functions during evolution, and participated in the response to multiple abiotic stresses. FRF genes are considered to be truncated FRS family proteins. They competed with FRS for DNA binding sites to regulate gene expression. However, only few studies are available on FRF genes in plants participating in the regulation of abiotic stress. With wide adaptability and high stress-resistance, barley is an excellent candidate for the identification of stress-resistance-related genes. In this study, 22 HvFRFs were detected in barley using bioinformatic analysis from whole genome. According to evolution and conserved motif analysis, the 22 HvFRFs could be divided into subfamilies I and II. Most promoters of subfamily I members contained abscisic acid and methyl jasmonate response elements; however, a large number promoters of subfamily II contained gibberellin and salicylic acid response elements. HvFRF9, one of the members of subfamily II, exhibited a expression advantage in different tissues, and it was most significantly upregulated under drought stress. In-situ PCR revealed that HvFRF9 is mainly expressed in the root epidermal cells, as well as xylem and phloem of roots and leaves, indicating that HvFRF9 may be related to absorption and transportation of water and nutrients. The results of subcellular localization indicated that HvFRF9 was mainly expressed in the nuclei of tobacco epidermal cells and protoplast of arabidopsis. Further, transgenic arabidopsis plants with HvFRF9 overexpression were generated to verify the role of HvFRF9 in drought resistance. Under drought stress, leaf chlorosis and wilting, MDA and O2− contents were significantly lower, meanwhile, fresh weight, root length, PRO content, and SOD, CAT and POD activities were significantly higher in HvFRF9-overexpressing arabidopsis plants than in wild-type plants. Therefore, overexpression of HvFRF9 could significantly enhance the drought resistance in arabidopsis. These results suggested that HvFRF9 may play a key role in drought resistance in barley by increasing the absorption and transportation of water and the activity of antioxidant enzymes. This study provided a theoretical basis for drought resistance in barley and provided new genes for drought resistance breeding

Temeljna študija o učinkovitosti na mestu izdelanega samostojnega betonskega pilota X prereza

X-section cast-in-place concrete (XCC) piles are used because they have a higher bearing capacity than circularsection cast-in-place concrete (CCC) piles of the same cross-sectional area. Although the bearing capacity of XCC piles has been studied, the performance of XCC single piles, especially for the stress-transfer mechanism dependent on the geometrical effects, is still not fully understood. This paper reports two comparative field static load tests on an XCC and a CCC single pile of the same cross-sectional area. In addition, corresponding threedimensional numerical back-analyses are performed to provide a fundamental understanding. The measured and computed results reveal that the XCC single pile has an approximately 25% higher ultimate bearing capacity than the CCC single pile. This is because the XCC single pile has an approximately 20% larger total side resistance, which is caused by a 60% larger pile perimeter and a slightly smaller unit side resistance. Lateral soil arching effects are developed, causing a non-uniform effective normal stress and a shear stress across the circumference of the XCC single pile. It is suggested that XCC single piles have a higher efficiency in terms of material saving compared with CCC single piles.Na mestu izdelani betonski piloti X prereza (XCC) se uporabljajo, ker imajo višjo nosilnost kot na mestu izdelani betonski piloti krožnega prereza (CCC) z enako površino prereza. Čeprav je bila preučevana nosilnost XCC pilotov, uspešnost samostojnih XCC pilotov, predvsem glede mehanizma prenosa napetosti v odvisnosti od geometrijskih učinkov, še vedno ni povsem jasna. V prispevku je podana primerjava terenskih statičnih obremenilnih preizkusov za XCC in CCC samostojna pilota z enako površino prečnega prereza. Za boljše razumevanje so bile dodatno izvedene tridimenzionalne numerične povratne analize. Izmerjeni in izračunani rezultati kažejo, da ima XCC samostojni pilot približno 25% višjo mejno nosilnost kot CCC samostojni pilot. To je zato, ker ima XCC samostojni pilot približno 20% višji skupni stranski odpor, ki je zaradi 60% večjega obsega prereza pilota in nekoliko manjše stranske odpornosti na enoto površine. V zemljini so se razvili bočni ločni učinki, ki povzročajo neenakomerno razporeditev efektivne normalne in strižne napetosti po obsegu XCC samostojnega pilota. Ugotovimo lahko, da imajo XCC samostojni piloti višjo učinkovitost z vidika privarčevanega materiala v primerjavi s CCC samostojnimi piloti

Superelastic Graphene Aerogel/Poly(3,4-Ethylenedioxythiophene)/MnO2 Composite as Compression-Tolerant Electrode for Electrochemical Capacitors

Ultra-compressible electrodes with high electrochemical performance, reversible compressibility and extreme durability are in high demand in compression-tolerant energy storage devices. Herein, an ultra-compressible ternary composite was synthesized by successively electrodepositing poly(3,4-ethylenedioxythiophene) (PEDOT) and MnO2 into the superelastic graphene aerogel (SEGA). In SEGA/PEDOT/MnO2 ternary composite, SEGA provides the compressible backbone and conductive network; MnO2 is mainly responsible for pseudo reactions; the middle PEDOT not only reduces the interface resistance between MnO2 and graphene, but also further reinforces the strength of graphene cellar walls. The synergistic effect of the three components in the ternary composite electrode leads to high electrochemical performances and good compression-tolerant ability. The gravimetric capacitance of the compressible ternary composite electrodes reaches 343 F g−1 and can retain 97% even at 95% compressive strain. And a volumetric capacitance of 147.4 F cm−3 is achieved, which is much higher than that of other graphene-based compressible electrodes. This value of volumetric capacitance can be preserved by 80% after 3500 charge/discharge cycles under various compression strains, indicating an extreme durability

Integration of G-Quadruplex and Pyrene as a Simple and Efficient Ratiometric Fluorescent Platform That Programmed by Contrary Logic Pair for Highly Sensitive and Selective Coralyne (COR) Detection

The effective and accurate detection of the anticancer drug coralyne (COR) is highly significant for drug quality control, medication safety and good health. Although various COR sensors have been reported in recent years, previous ones can only exhibit single-signal output (turn ON or turn OFF) with poor reliability and anti-interference ability. Therefore, exploring novel platform with dual-signal response for COR detection is urgently needed. Herein, we reported the first ratiometric fluorescent platform for highly sensitive and selective COR detection by integrating G-quadruplex (G4) and Pyrene (Py) as signal probes and harnessing A-COR-A interaction. In the absence of COR, the platform shows a low fluorescence signal of PPIX (F642) and a high one of Py monomer (F383). With the addition of COR, two delicately designed poly-A ssDNAs will hybridize with each other via A-COR-A coordination to form complete G4, yielding the increased fluorescence signal of PPIX and the decreased one of Py due to the formation of Py excimer. Based on the above mechanism, we constructed a simple and efficient sensor that could realize the ratiometric fluorescent detection of COR with high sensitivity and selectivity. A linear relationship between F642/F383 and COR’s concentration is obtained in the range from 1 nM to 8 μM. And the limit of detection of COR could reach to as low as 0.63 nM without any amplification, which is much lower than that of most COR sensors reported so far. Notably, the logical analysis of COR can be carried out under the control of a “YES-NOT” contrary logic pair, enabling the smart dual-channel response with an adequate S/N ratio and improved reliability and anti-interference ability. Moreover, this system also presents satisfactory performance in fetal bovine serum (FBS) samples

Isolation and characterization of a marine bacterium Vibrio diabolicus strain L2-2 capable of biotransforming sulfonamides

Sulfonamides (SAs) have attracted much attention because of their high detection rates in natural water. In this study, a marine bacterium Vibrio diabolicus strain L2-2 was isolated which could metabolize 9 SAs to a different extent. Compared with SAs and their analogs, SAs with N-oxides of heterocyclic structure were easier to be transformed to their N-4-acetylated metabolites or their isoxazole ring rearrangement isomers by strain L2-2. And, gene vdnatA and vdnatG were likely to be the key genes in SAs acetylation process, which might code Arylamine N-acetyltransferase. The biotransformation rates of sulfathiazole(STZ), sulfamonomethoxine(SMT), sulfadiazine (SDZ), sulfamethoxazole(SMX) and sulfisoxazole(SIX) could reach 29.39 +/- 5.63, 24.97 +/- 4.45, 79.41 +/- 4.05, 64.64 +/- 1.71, 32.82 +/- 4.46% in 6 days, respectively. Besides, the overall optimal conditions for SAs biotransformation were less than 100 mg/L for total SAs in neutral or weakly alkaline medium with the salinity of 10-20%o and additional nutrients like glucose, sucrose or glycerine. Furthermore, toxicity was demonstrated to be significantly reduced after biotransformation. Together, this study introduced a strategy to use V. diabolicus strain L2-2 to realize simultaneous removal and detoxification of multiple SAs in freshwater and seawater, and revealed SAs removal pathways and relevant molecular mechanism

The Influencing Factors of Pro-Environmental Behaviors of Farmer Households Participating in Understory Economy: Evidence from China

To promote the sustainable development of agriculture and forestry economy, it is of great significance to guide farmers to consciously pursue pro-environmental behaviors in the development of the understory economy. Based on field survey data from Yong’an city of Fujian Province and Luoshan County of Henan Province, factor analysis and Oprobit models were mainly used to analyze the influencing factors and influencing the degree of the pro-environmental behaviors of farmer households participating in the understory economy. Quantitative data showed that individual characteristics of farmers (i.e., gender, education, career, feasibility evaluation, and the proportion of farming labor to household labor) and forest land management status (i.e., forest land transfer, the working time in understory economy, and proportion of understory economic income) have an obvious effect on the adoption of pro-environmental behaviors. Findings also revealed three key variables (i.e., the farmers’ environmental perception, social constraints, and government incentives) that are associated with the willingness to adopt pro-environmental behaviors. The contribution ratios of the influencing factors were environmental perception, social constraints, and government incentives. In addition to economic benefits, perceptual factors and informal institutions also play an important role in driving farmers to adopt pro-environmental behaviors. Based on the findings, it is necessary to strengthen the publicity and the education of farmers, increase environmental responsibility, accelerate the establishment and improvement of ecological reward-and-punishment mechanisms, and enhance the training of green production techniques

Biotransformation mechanism of Vibrio diabolicus to sulfamethoxazole at transcriptional level

Sulfamethoxazole (SMX) has attracted much attention due to its high probability of detection in the environment. Marine bacteria Vibrio diabolicus strain L2-2 has been proven to be able to transform SMX. In this study, the potential resistance and biotransformation mechanism of strain L2-2 to SMX, and key genes responses to SMX at environmental concentrations were researched. KEGG pathways were enriched by down-regulated genes including degradation of L-Leucine, L-Isoleucine, and fatty acid metabolism. Resistance mechanism could be concluded as the enhancement of membrane transport, antioxidation, response regulator, repair proteins, and ribosome protection. Biotransformation genes might involve in arylamine N-acetyltransferases (nat), cytochrome c553 (cyc-553) and acyl-CoA synthetase (acs). At the environmental concentration of SMX (0.1-10 mu g/L), nat was not be activated, which meant the acetylation of SMX might not occur in the environment; however, cyc-553 was up-regulated under SMX stress of 1 mu g/L, which indicated the hydroxylation of SMX could occur in the environment. Besides, the membrane transport and antioxidation of strain L2-2 could be activated under SMX stress of 10 mu g/L. The results provided a better understanding of resistance and biotransformation of bacteria to SMX and would support related researches about the impacts of environmental antibiotics

A modified analytical solution of soil stress distribution for XCC pile foundations

X-section cast-in-place concrete pile (XCC pile) is a new type of pile foundation, which has an X-shaped cross section. Compared to the traditional circular pile of the same cross-sectional area, the bearing capacity of an XCC pile is higher due to increased cross-sectional perimeter. Since Geddes solution is based on St. Venant's principle, leading to the results independent of the cross-sectional geometry and size, large differences are induced when estimating the soil stress distribution for XCC pile foundations. This paper derives a modified analytical solution, which is dependent on the cross-sectional geometry of XCC pile, from Geddes solution. Validation of this modified solution was conducted through three-dimensional numerical analysis and proven more suitable for XCC pile foundations. Parametric study on three geometrical parameters is conducted using this modified solution. The results indicate that the stress in founding soil due to skin friction decreases with increasing pile radius and central angle of concave, but increases with increasing length of flat side. The stress due to end-bearing decreases with increasing pile radius and length of flat side, but increases with increasing central angle of concave. From the parametric studies, the recommended dimensions of XCC pile radius, length of flat side, and central angle of concave are recommended ranges from 200 to 600 mm, 30 to 60 mm, and 90 degrees to 150 degrees, respectively.GUNAWAN, Anthony, CIVL, SENG, HKUST. [email protected]