Clinical application of 8-0 polypropylene scleral-sutured fixed intraocular lens suspension implantation with the double knots technique in aphakic eyes

AIM: To observe the clinical efficacy of 8-0 polypropylene scleral-sutured fixed intraocular lens(IOL)suspension implantation with the double knots technique in aphakic eyes.METHODS: Retrospective case series study. The data of 30 aphakic cases(31 eyes, 22 males)that underwent IOL suspension in our hospital from January 2021 to November 2022 were collected. The suspension of IOL(AcrySof IQ or Tecnis ZCB00)was performed by 8-0 polypropylene scleral-sutured with the double knots technique. The visual acuity, intraocular pressure(IOP), IOL position and complications with at least 6 mo of follow-up were observed.RESULTS: The mean preoperative uncorrected visual acuity(UCVA, LogMAR)and best-corrected visual acuity(BCVA, LogMAR)were 2.53±0.78 and 0.35±0.26, respectively, which were 0.58±0.26 and 0.36±0.27 at 6 mo postoperatively, respectively. And the differences in UCVA were statistically significant(t=15.408, P<0.01), whereas the difference in BCVA was not(t=-1.677, P=0.104). There were no intraoperative complications, with IOL position all centered, but 3 eyes had IOL tilt, 2 eyes had intraocular hypertension, 5 eyes had corneal edema, and 1 eye had suture exposure postoperatively. There were no complications such as hyphema, vitreous hemorrhage, macular edema, corneal endothelial decompensation, hypotony, choroidal detachment, retinal detachment, fulminant superior choroidal hemorrhage, endophthalmitis, or others.CONCLUSION: The 8-0 polypropylene scleral-sutured fixed intraocular lens suspension implantation with the double knots technique can improve the postoperative visual acuity of aphakic patients, and fewer complications, which is an option for the treatment of aphakia, dislocation of the lens and ligament abnormalities

Open-Circuit Fault Diagnosis of Three-Phase PWM Rectifier Using Beetle Antennae Search Algorithm Optimized Deep Belief Network

Effective open-circuit fault diagnosis for a two-level three-phase pulse-width modulating (PWM) rectifier can reduce the failure rate and prevent unscheduled shutdown. Nevertheless, traditional signal-based feature extraction methods show poor distinguishability for insufficient fault features. Shallow learning diagnosis models are prone to fall into local extremum, slow convergence speed, and overfitting. In this paper, a novel fault diagnosis strategy based on modified ensemble empirical mode decomposition (MEEMD) and the beetle antennae search (BAS) algorithm optimized deep belief network (DBN) is proposed to cope with these problems. Initially, MEEMD is applied to extract useful fault features from each intrinsic mode function (IMF) component. Meanwhile, to remove features with redundancy and interference, fault features are selected by calculating the importance of each feature based on the extremely randomized trees (ERT) algorithm, and the dimension of fault feature vectors is reduced by principal component analysis. Additionally, the DBN stacked with two layers of a restricted Boltzmann machine (RBM) is selected as the classifier, and the BAS algorithm is used as the optimizer to determine the optimal number of units in the hidden layers of the DBN. The proposed method combined with feature extraction, feature selection, optimization, and fault classification algorithms significantly improves the diagnosis accuracy

Effect of forceful suction and air disinfection machines on aerosol removal

Abstract Backgrounds Dental procedures involving drilling and grinding can produce a significant amount of suspended aerosol particles (PM) and bioaerosols. This study aims to analyze the size and concentration of aerosol particles generated during drilling and to investigate the effectiveness of two air exchange systems, namely forceful suction (FS) and air disinfection machines (DM), in removing PM. Methods For this study, 100 extracted permanent teeth were collected and divided into three groups: without suction (n = 50), suction with forceful suction (n = 25), and suction with air disinfection machines (n = 25). The removal rate of suspended aerosol particles was analyzed using particle counters and air data multimeter. Results When drilling and grinding were performed without vacuum, 0.75% of the aerosol particles generated were PM2.5-10, 78.25% of total suspended aerosol particles (TSP) were PM2.5, and 98.68% of TSP were PM1. The nanoanalyzer measurements revealed that the aerodynamic diameter of most aerosol particles was below 60 nm, with an average particle diameter of 52.61 nm and an average concentration of 2.6*1011 ultrafine aerosol particles. The air change per hour (ACH) was significantly lower in the air disinfection machines group compared to the forceful suction group. Additionally, the number of aerosol particles and mass concentration was significantly lower in the air disinfection machines group compared to the forceful suction group in terms of PM2.5 levels. However, the forceful suction group also reduced the mass concentration in PM10 level than the air disinfection machines group. Conclusion In conclusion, the air exchange system can reduce the aerosol particles generated during drilling and grinding. Comparing the two air exchange systems, it was found that the air disinfection machines group reduces the number of aerosol particles and mass concentration in PM2.5 levels, while the forceful suction group reduces the mass concentration in PM10 level

Generation of reactive oxygen species from oxygen microbubbles in phosphoric acid solution and its application of ferrous iron oxidation

Background: Microbubbles have been widely used in advanced oxidation processes due to the generation of reactive oxygen species (ROS). In this study, the oxidation of ferrous ions in phosphoric acid solutions has been examined, focusing on the advanced oxidation activity of oxygen microbubbles. Methods: Ultraviolet spectroscopy was used to determine the species and content of ROS. Electron spin-resonance spectroscopy and radical quenching experiments have confirmed the path of ROS generation. Significant findings: It is concluded that the main ROS in the phosphoric acid solution was H2O2, and the generation of H2O2 was due to sequential single electron reduction of oxygen molecules. The amount of H2O2 generated was determined to be dependent on the temperature and phosphoric acid concentration, and under the optimal conditions (100 degrees C and 7 mol/L H3PO4), 4.23 mmol/L H2O2 was produced from microbubbles. The kinetics analysis with respect to the oxidation of ferrous ions suggested significant reduction of reaction activation energy from greater than 56.1kJ/mol to 43.84 kJ/mol due to the oxidation via ROS, demonstrating that microbubble intensification is a facile and effective advanced oxidation method. (c) 2021 Taiwan Institute of Chemical Engineers. Published by Elsevier B.V. All rights reserved

Genome sequence of Aspergillus flavus A7, a marine-derived fungus with antibacterial activity

Due to the specific properties of the marine environment, marine microorganisms have exclusive physicochemical characteristics that are different from those of terrestrial microorganisms, which can produce various secondary metabolites (SMs) with considerable structural diversity and biological activity. In this study, three strains of coepiphytic Aspergillus with potential antibacterial activities, A7 (Aspergillus flavus), B27 (Aspergillus flavipes) and R12 (Aspergillus sydowii), were isolated from the South China Sea. Via the Illumina MiSeq sequencing platform, the genomes of the three strains were sequenced, and genome comparison showed the highest diversity of the biosynthetic gene clusters (BGCs) in A7. Meanwhile, a comparison of physiological and genomic characteristics between A7 and other Aspergillus flavus strains demonstrated the superior environmental adaptability of A7, which is apparently consistent with the genetic richness of BGCs. By assigning reads to known BGCs, putative BGCs were allocated in A7 that corresponded to various SMs, including naphthopyrone, pyranonigrin E, cyclopiazonic acids, etc. Based on gene homology analysis, we surmise that a region is involved in the biosynthesis of ustiloxin-like RiPPs, a less thoroughly studied SM in fungi. Our results provide genetic information for the investigation of marine Aspergillus sp., which may help to elucidate their chemical diversity and adaptive strategies.The accepted manuscript in pdf format is listed with the files at the bottom of this page. The presentation of the authors' names and (or) special characters in the title of the manuscript may differ slightly between what is listed on this page and what is listed in the pdf file of the accepted manuscript; that in the pdf file of the accepted manuscript is what was submitted by the author

Potassium Hydroxide Concentration-Dependent Water Structure on the Quartz Surface Studied by Combining Sum-Frequency Generation (SFG) Spectroscopy and Molecular Simulations

Vibrational sum-frequency generation (SFG) spectroscopy and molecular simulations were used to investigate the molecular structures at the quartz surface, and the influence of bulk potassium hydroxide concentration was systematically examined. It was found that when the potassium hydroxide concentration was less than 10(-2) M, the structure of water molecules at the quartz surface was dependent on the quartz surface potential as evidenced by the increase of SFG signal as a function of the alkaline concentration. However, when the alkaline concentration was more than 10( )(-2)M, a monotonic decrease of interfacial water SFG spectra intensity was observed, which has been proposed to be due to the decreased number of interfacial water molecules and proton disordering caused by the screening effect originated from the adsorption of cations. Furthermore, besides the typical hydrogen-bonded interfacial water peaks (3200 and 3400 cm(-1)), the quartz/H2O interface showed an additional red-shifted peak centered at similar to 2930 cm(-1). The results of SFG spectra and chemistry calculations confirmed that the red-shifted vibrational peak was due to the O-H stretch vibration of water molecules strongly hydrogen bonded with the OH- adsorbed at the surface

Molecular Characterization of a New Alkaline-Tolerant Xylanase from Humicola insolens Y1

An endo-1,4-β-xylanase-encoding gene, xyn11B, was cloned from the thermophilic fungus Humicola insolens Y1. The gene encodes a multimodular xylanase that consists of a typical hydrophobic signal sequence, a catalytic domain of glycoside hydrolase (GH) family 11, a glycine-rich linker, and a family 1 carbohydrate binding module (CBM1). Deduced Xyn11B shares the highest identity of 74% with a putative xylanase from Podospora anserina S mat+. Recombinant Xyn11B was successfully expressed in Pichia pastoris and purified to electrophoretic homogeneity. Xyn11B had a high specific activity of 382.0 U mg−1 towards beechwood xylan and showed optimal activity at pH 6.0 and 50°C. Distinct from most reported acidic fungal xylanases, Xyn11B was alkaline-tolerant, retaining 30.7% of the maximal activity at pH 9.0. The Km and Vmax values for beechwood xylan were 2.2 mg mL−1 and 462.8 μmol min−1 mg−1, respectively. The enzyme exhibited a wider substrate specificity and produced a mixture of xylooligosaccharides. All these favorable enzymatic properties make Xyn11B attractive for potential applications in various industries