Development of the Multi-Analyte Test for Immune-Chromatographic Detection of Botulinum Toxins

Designed is the multi-analyte test for simultaneous immune chromatographic detection of A & B type botulinum neurotoxins (BT), using colloid gold nanoparticles. It is meant for food and environmental samples' analysis. The sensitivity of simultaneous BT detection of the A (30 ng/ml) and B (10 ng/ml) types is as high as that of mono-analytical tests, designed for one type BT detection. The test is demonstrated to be a specific one and can be used for BT detection in food stuffs

Construction of Immune-Chromatographic Indicator Elements for Burnet Rickettsia Detection

mc/ml, and the elapsed time – 20 minutes. The IE is specific to other members of the family Ricketsiaceae, for instance to R. prowazekii , to antigen complexes of R. prowazekii and R. sibirika , as well as to vaccinia virus (L-IVP strain). The IE engineered can be used for rapid indication of Burnet Rickettsia at different stages of laboratory investigation. Span time reduction, lack of necessity to perform any accessory technological operations, visual and (or) automatic registration of the results build up premises to observe immune-chromatographic method for Burnet Rickettsia detection as one of the alternatives for identification of these microorganisms under field conditions when monitoring ambient environment objects

Modern tendencies of diagnostics and treatment of acute coronary syndromes on the stage of medical first-aid

The purpose of work is generalization of the available data of literature about the modem being of diagnostics and treatment of acute coronary syndromes on the stage of medical first-aid. Basic principles of pathogenetic therapy of acute ST- segment elevation myocardial infarction (STEMI) are considered, experience of the use of markers of myocardial necrosis is presented, efficiency of the combined antiplatelet therapy is shown in treatment of acute coronary syndromes.Целью работы является обобщение имеющихся данных литературы о современном состоянии диагностики и лечения острого коронарного синдрома на догоспитальном зтапе скорой медицинской помощи. В работе рассмотрены основные принципы патогенетической терапии острого коронарного синдрома с подъемом сегмента ST, представлен опыт использования биомаркеров некроза миокарда на догоспитальном этапе, показана эффективность комбинированной антитромботической терапии в лечении острых коронарных синдромов

Crystallographic Features of Phase Transformations during the Continuous Cooling of a Ti6Al4V Alloy from the Single-Phase β-Region

Crystallographic relationships between α- and β-phases resulting from phase transformations, which took place during the continuous water quenching (WQ), air cooling (AC) and furnace cooling (FC) of a Ti6Al4V plates solution treated at 1065 °C, were investigated by methods of electron backscatter diffraction (EBSD) and transmission electron microscopy (TEM). WQ, AC and FC resulted in typical martensite, basket-weave and parallel-plate Widmanstatten structures, respectively. The experimental distribution of α/β-misorientations deviated from BOR at set discrete angles close to 22, 30, 35 and 43°. The experimental spectra of angles were confirmed by theoretical calculations of the possible misorientations between the α and β phases through the βo→α→βII –transformation path based on Burgers orientation relationship (BOR). Joint analysis of the experimental data and theoretical calculations revealed that the secondary βII-phase was precipitated according to the sequence βo→α→βII during continuous cooling from the single-phase β-region. Similar spectra for α/β-phase misorientations for all investigated cooling rates acknowledged the similar transformation mechanisms and dominant shear component of the phase transformations. © 2022 by the authors.Ministry of Education and Science of the Russian Federation, MinobrnaukaThis research was funded by the Ministry of Science and Higher Education of the Russian Federation (Ural Federal University Program of Development within the Priority-2030 Program)

Research Grain Structure of Zr–2,5 % Nb Alloy Tubes by SEM Methods

The research was performed on thin sections of the transverse and longitudinal sections of tubes with a diameter of 88 mm from an alloy of Zr–2,5 %Nb, which have a fine-grained structure. Using scanning electron microscopy (SEM), the average grain size and structural components of the alloy after manufacture are determined. EBSD analysis compiled maps of preferred crystallographic orientations.Проведено исследование на шлифах поперечного и продольного разреза трубного изделия диаметром 88 мм из сплава Zr–2,5 %Nb, обладающих мелкозернистой структурой (~ 1–2 мкм). Методами сканирующей электронной микроскопии (СЭМ) определены структурные составляющие сплава (твердый раствор α-Zr и частицы β-Nb), а также средний размер зерен α-Zr. Ориентационным анализом (EBSD) составлены карты преимущественных кристаллографических ориентировок

Development of Multi-Analytical Immune-Chromatographic Device for Toxin Indication

Objective of the work was to construct immune-chromatographic device for indication of five toxin types in a single processing cycle and investigation of its capacities regarding environment sample analysis and food testing. Obtained were the conjugates of monoclonal antibodies with nano-particles of colloid gold, formed were membrane composites for sandwich immune-chromatography of separate toxins. On the basis of distinct test panels designed was immune-chromatographic device for multiple analyses, which contained multi-analytical detection element, swab for surface sampling, and storage vessel with test buffer. This device allowed for concurrent identification of botulinic toxins A, B types concentrated up to 5 and 10 ng/ml, respectively; staphylococcal toxin B type - 40 ng/ml, cholera toxin - 500 ng/ml, and ricin - 80 ng/ml, within a single processing cycle. Demonstrated was the possibility of toxin detection in washings from ambient object surfaces, as well as in foodstuffs. Described was preliminary food sample preparation procedure

Modern strategies in reducing the risk of death from acute coronary syndrome

The aim of this work is to summarize the available literature data about modern treatment strategies acute coronary syndromes, their effectiveness in reducing the risk of severe complications and death. The paper describes the main principles of pathogenetic therapy of acute coronary syndrome with ST- segment elevation, presents modern strategy of reperfusion therapy, the effectiveness of combination antiplatelet therapy. Shows the role of prehospital care in reducing risk of death in implementing pharmacoinvasive strategy.Целью работы является обобщение имеющихся данных литературы о современных стратегиях лечения острых коронарных синдромов, их эффективности в снижении риска тяжелых осложнений и смерти. В работе рассмотрены основные принципы патогенетической терапии острого коронарного синдрома с подъемом сегмента ST, представлены современные стратегии реперфузионной терапии, показана эффективность комбинированной антитромботической терапии в лечении острых коронарных синдромов. Показана роль догоспитального этапа в снижении риска смерти при реализации фармакоинвазивной стратегии

Structure Alloy Steel Cr12–Ni–Mo–W–Nb–V–Si–B Quenched-and-Tempered

Scanning of electronic microscopy is made, including with use EBSD the structure has been investigated became after training and high tempering. The phase structure, morphology δ — ferrite, martensite, large carbides NbС, and as presence in a product expressed crystallographic structures became established.Сканирующей электронной микроскопией, в том числе с использованием ориентационного анализа (EBSD), была исследована структура стали Х12НМВБФСР после закалки и высокого отпуска. Установлены фазовый состав стали и морфология элементов структуры: δ-феррита, мартенсита, карбидов NbС, а также наличие в изделии выраженной кристаллографической структуры

The Orientation Microscopy Possibilities in the Analysis of Grain Structure in Low Carbon Steels after TMCP

The method of orientation microscopy (EBSD) is used to study the grain structure of low-carbon, low-alloy pipe steel sheets processed by controlled thermomechanical processing (TMCP). The possibilities of obtaining different grain characteristics depending on the specified analysis limitations are shown.Методом ориентационной микроскопии проанализирована зеренная структура образцов малоуглеродистой низколегированной трубной стали, полученных контролируемой термомеханической обработкой. Показаны возможности получения различных зеренных характеристик в зависимости от заданных «граничных» условий анализа.Работа выполнена при поддержке стипендии Президента Российской Федерации, проект СП-259.2018.1

EFFECT OF GD ON THE STRUCTURE OF NEAR-α TITANIUM ALLOY

The effects of gadolinium on the structure of the pseudo-α titanium alloy VT18U after standard heat treatments were studied by methods of metallographic analysis using an optical microscope, transmission and scanning electron microscopy. It was shown that intermetallic particles based on gadolinium and tin and particles of gadolinium oxide are present in the alloy after the treatments.Методами металлографического анализа с помощью оптического микроскопа, просвечивающей и растровой электронной микроскопии исследовано влияние гадолиния на структуру псевдо-α титанового сплава ВТ18У после стандартных термических обработок. Показано, что после обработок в сплаве присутствуют интерметаллидные частицы на основе гадолиния и олова и частицы оксида гадолиния