Tamoxifen and mifepriston modulate nicotine induced conditioned place preference in female rats

WOS: 000289958600009PubMed ID: 21272620An increasing number of studies suggest that nicotine/tobacco addiction is modulated by ovarian hormones. The levels of estrogen and progesterone appear to be important in the success of quit attempts and smoking cessation. In women smokers with the diagnosis or risk of breast cancer, the estrogen receptor modulator tamoxifen (TAM) is widely used, and even though the detrimental health effects of smoking are known, this vulnerable group has difficulty quitting and continues to smoke. The current study tested the effect of the estrogen receptor modulator TAM and the progesterone receptor antagonist mifepriston (RU486) on nicotine-induced conditioned place preference (CPP) in adult female rats. A three chambered CPP apparatus was used and nicotine was paired with the initially non-preferred chamber. Rats received nicotine or saline and hormone receptor modulators (vehicle, TAM, RU486) in a 2 x 3 experimental design. We have previously shown that nicotine induces CPP in male Sprague-Dawley rats but not in females. Our results show that while nicotine alone does not induce CPP in female rats, rats treated with TAM exhibit nicotine-induced CPP. Although RU486 has an aversive effect when applied alone, this is ameliorated by nicotine. These results confirm the role of ovarian hormone receptors in nicotine-induced CPP and may have clinical implications for developing more efficient smoking cessation approaches in women smokers. (C) 2011 Elsevier Inc. All rights reserved.Ege UniversityEge University [2006-BAM-02]This work was supported by Ege University Research Fund (Grant Number: 2006-BAM-02)

The association between probationers’ addiction levels and socioeconomic-psychological characteristics

Abstract Objective The aim of this study is to evaluate probationers’ addiction levels and associated socioeconomic and psychological features in Izmir Probation Directorate. Methods This cross-sectional study was conducted in Izmir Probation Directorate between August 27, 2018 and November 27, 2018. The study’s dependent variable was adult probationers’ addiction level which was evaluated by the Addiction Profile Index-Clinical Version (API-C). The independent variables were individual factors, social environmental factors, API-C scale subdimensions and perceived social support. For paired comparisons, Student’s t test and ANOVA were used. Linear regression analysis was used for multiple comparisons. p < 0.05 was accepted as the limit of statistical significance. Results A total of 200 male probationers participated in the study (82.3%, n = 243). The participants’ average age was 29.9 ± 7.7. The participants’ average addiction score was 5.65 ± 2.33. According to the results of the regression analysis; education level (B = 1.438, 95% CI 0.936, 1.941, p < 0.001) working status at a job (B = 2.687, 95% CI 1.428, 3.945, p < 0.001) father’s education level (B=-1.117, 95% CI -1.473, -0.762, p < 0.001) and anger management problems (B = 0.750, 95%CI 0.517, 0.982, p < 0.001) were explanatory for addiction level. The model was 50.8% explanatory of addiction level (p < 0.05). Conclusion Probationers who grew up with only their mother, whose father had a low education level, who had higher levels of anger management problems and who were unemployed had higher levels of addiction. These results emphasize the need for social work in the rehabilitation processes of individuals. Treating the risk factors indicated by the study results as screening and follow-up parameters in the probation population can be useful in improving the success of the probation program

Previous chronic exposure eliminates the conditioning effect of nicotine in rats

WOS: 000293719700004PubMed ID: 21640169Smoking continues to be a major health problem and unfortunately smoking cessation interventions have limited success; the conditioning effects of nicotine and individual differences in tobacco addiction are important factors that underlie this setback. The aim of the current study was to investigate nicotine-induced conditioned place preference (CPP) in male and female rats which were previously exposed to a free choice of oral nicotine or water and showed different preferences for nicotine; subsequently nicotine intake also varied between subjects. Exposure patterns were varied in three experiments to allow for assessing the effect of adult v.s. adolescent exposure. The design of CPP testing enabled testing for the possible confounding effects of withdrawal or tolerance. A total of 150 male and female rats were used in three experiments. The oral nicotine choice was provided for at least six weeks in all experiments. Our results replicate our previous findings that nicotine induces CPP in male, but not female rats not pre-exposed to nicotine. Previous nicotine exposure, irrespective of the amount of nicotine consumed, eliminated the conditioning effects of nicotine in a new context. The diminished CPP response was more pronounced in rats exposed to nicotine as adolescents than those exposed as adults. This reduced responsiveness cannot be explained by tolerance. The neuroplastic changes caused by chronic nicotine administration or the strong conditioning to receiving nicotine in the home cage before CPP testing may underlie the weakened responsiveness. These findings support the well known clinical notion that smoking cessation attempts are more successful in a novel environment, not previously connected with smoking. (C) 2011 Elsevier Inc. All rights reserved.Ege UniversityEge University [001 BAM 2006-02]This work was supported by Ege University Research Fund (Grant 001 BAM 2006-02)

CART expression in limbic regions of rat brain following forced swim stress: Sex differences

WOS: 000238162000004PubMed ID: 16644010Our previous studies showed the modulation of cocaine and amphetamine regulated transcript (CART) positive neurons and CART mRNA by adrenalectomy and corticosterone replacement in hypothalamic nuclei of male rat brain. More recently, we have shown by CART immunohistochemistry that restraint and forced swim (FS) stress have sexually dimorphic and regionally specific effects on CART expression in the hypothalamic nuclei of male and female Sprague-Dawley rats. This study aimed to evaluate the effects of FS stress on CART peptide expression in hypothalamus, amygdala and hippocampus of male and female (in or near estrus) Sprague-Dawley rats. Initially basal CART levels in regions of interest were determined in male and female rats; no sex differences were observed. In FS test, rats were forced to swim on two consecutive days, in a Plexiglas cylinder for 15 and 6 min, respectively. Rats were decapitated on the second day, 10 min after the stress procedure. Hypothalami, amygdalae and hippocampi were dissected and homogenized. CART peptide expression in these regions was measured by Western blotting. In males, FS increased CART expression in hypothalamus and amygdala. On the other hand, in females, FS lowered CART expression in amygdala. CART expression in hippocampus was not affected by the stress procedure in either sex. Our results suggest sexually dimorphic modulation of CART expression in hypothalamus and amygdala by FS procedure. Although modulation of the CART peptide by glucocorticoids and gonadal hormones appears likely, future studies are needed to elucidate the underlying mechanisms in the involvement of CART peptide in stress response. (c) 2006 Elsevier Ltd. All rights reserved.NCRR NIH HHSUnited States Department of Health & Human ServicesNational Institutes of Health (NIH) - USANIH National Center for Research Resources (NCRR) [RR00165]; NIDA NIH HHSUnited States Department of Health & Human ServicesNational Institutes of Health (NIH) - USANIH National Institute on Drug Abuse (NIDA) [DA00418, 3 R01 DA010732-05S1

Smoke-free Ege: An attempt for an integrative prevention strategy for tobacco control at a University setting

Introduction Ege University is a pioneering university at Ege region with its 70 000 students and approximately 3000 academic staff. Tobacco control policies, parallel to the current regulations in the country, are an important issue for Ege University too, yet it bares important opportunities as well as barriers for an integrative approach for tobacco control on University Campus. Aim This study aims to explain the construction of Smoke-Free Ege Working Group, its formal initiation at the World No Tobacco Day (May 31st) and its subsequent action plan structured in the light of MPOWER. Methods Since 1999, smoking cessation outpatient service delivery points have been the main units of motivation for tobacco control policies at Ege University. Although Turkey is moving well about tobacco regulations, adherence to regulations is deficient and high prevalence of smoking among university staff and students remains as a major concern throughout the years. In March 2018, the administrative bodies of Medical School, have dealt their concerns to the units serving care for cessation and have gathered them at a first meeting. The Dean and Medical Manager of Medical Faculty, with a non-smoker administrative team, mentored and facilitated these efforts. Starting at this point, with regular meetings, the representatives determined their objectives, current barriers and opportunities. The construction of Smoke-Free Ege Working Group has rooted from these consecutive meetings. In the light of MPOWER, specific objectives at University Campus were listed. The major aim of the group was recognized as “changing the norms and culture for smoking”. This needed a good promotion strategy as well as collaboration with staff, students and administrative units. The efforts were collaborated with the Communications Unit of the University. A logo was created, brochures to raise awareness for the Smoke Free issue and the Working group, were prepared. The formal presentation of the Working Group was planned as a colorful activity on World No Tobacco Day, May 31st. To gather more visibility at the social media and press, representatives from three major opponent sports teams of Izmir city were invited. Results The Working Group was introduced to the media in the presence of the Rector, Dean, Medical managerl and all administrative units of the Medical School. Staff who succeeded to stop smoking, at the cessation service of the University, were awarded with certificates. The media showed great interest in the activity as it was put as “the famous sportsmen are supporting the efforts for a Smoke-free university”. Students and staff attended and shared the activity in social media as the entrance of the meeting was also colorfully designed for such promotion. Conclusions The Working Group has achieved to attract attention of staff, students and responsible bodies of the University and now moving forward for Smoke –Free Councils with students and staff representatives. Barriers and opportunities will be discussed, actions in the light of MPOWER, will be planned at these Councils. Smoke –Free Unit certifications and awards, designing smoking points outside of education and hospital settings and changing the “norm” are targeted

Prevalence and clinical/molecular characteristics of PTEN mutations in Turkish children with autism spectrum disorders and macrocephaly

Background: Phosphatase and tensin homolog (PTEN) germline mutations are associated with cancer syndromes (PTEN hamartoma tumor syndrome; PHTS) and in pediatric patients with autism spectrum disorder (ASD) and macrocephaly. The exact prevalence of PTEN mutations in patients with ASD and macrocephaly is uncertain; with prevalence rates ranging from 1% to 17%. Most studies are retrospective and contain more adult than pediatric patients, there is a need for more prospective pediatric studies