Study of “Catalytic Functions” of the “Friend” Roles of The Analects in Business Dealings

In international business dealings, we inevitably encounter problems arising from cultural obstacles, which in business dealings influence us greatly. However, the influence of cultural barriers can be decreased by the role of friends in business dealings. Also, the discussion of the “friend” roles of The Analects gives us theoretical basis for helping us to overcome cultural barriers. Previous research has showed that nowadays business dealings are affected by cultural obstacles, but it is found that the “friend” roles of The Analects can help us, to some extent, to remove the obstacles in business dealings. This paper cited many examples of the “friend” roles of The Analects, and their excellent characters are illustrated. These characters help us to promote the common cause, strengthen business relations, extend the partnership, and enjoy the cooperation in business dealings. Therefore, according to the “friend” roles of The Analects, we can stimulate “catalytic functions” in business dealings

Mixed Mortar Element Method for P NC 1 /P 0 Element and Its Multigrid Method for the Incompressible Stokes Problem

We discuss a mortar-type P NC 1 /P 0 element method for the incompressible Stokes problem. We prove the inf-sup condition and obtain the optimal error estimate. Meanwhile, we propose a W-cycle multigrid for solving this discrete problem and prove the optimal convergence of the multigrid method, that is, the convergence rate is independent of the mesh size and mesh level. Finally, numerical experiments are presented to confirm our theoretical results

Protein kinase Cα downregulation via siRNA-PKCα released from foldable capsular vitreous body in cultured human retinal pigment epithelium cells

We previously found that downregulation of protein kinase Cα (PKCα) can inhibit retinal pigment epithelium (RPE) cell proliferation involved in the development of proliferative vitreoretinopathy (PVR). In this study, we tested whether PKCα could be downregulated via small interfering RNA (siRNA)-PKCα released from foldable capsular vitreous body (FCVB) in cultured human RPE cells. SiRNA-PKCα content, determined by ultraviolet (UV) spectrophotometer, was released from FCVB containing 200, 300, 400, 500, and 600 nm siRNA-PKCα in a time-dependent manner from 1 to 96 hours and a dose-dependent manner at five concentrations. The content (y) had a good linear relationship with time (x), especially in the 600 nm siRNA-PKCα group (y = 16.214x, R2 = 0.9809). After treatment with siRNA-PKCα released from FCVBs, the PKCα was significantly decreased by RT-PCR, Western blot, and immunofluorescence analysis in RPE cells. These results indicate that PKCα was significantly downregulated by siRNA-PKCα released from FCVB in human RPE cells and provide us with a new avenue to prevent PVR

BMP4 inhibits myogenic differentiation of bone marrow–derived mesenchymal stromal cells in mdx mice

AbstractBackground aimsBone marrow–derived mesenchymal stromal cells (BMSCs) are a promising therapeutic option for treating Duchenne muscular dystrophy (DMD). Myogenic differentiation occurs in the skeletal muscle of the mdx mouse (a mouse model of DMD) after BMSC transplantation. The transcription factor bone morphogenic protein 4 (BMP4) plays a crucial role in growth regulation, differentiation and survival of many cell types, including BMSCs. We treated BMSCs with BMP4 or the BMP antagonist noggin to examine the effects of BMP signaling on the myogenic potential of BMSCs in mdx mice.MethodsWe added BMP4 or noggin to cultured BMSCs under myogenic differentiation conditions. We then injected BMP4- or noggin-treated BMSCs into the muscles of mdx mice to determine their myogenic potential.ResultsWe found that the expression levels of desmin and myosin heavy chain decreased after treating BMSCs with BMP4, whereas the expression levels of phosphorylated Smad, a downstream target of BMP4, were higher in these BMSCs than in the controls. Mdx mouse muscles injected with BMSCs pretreated with BMP4 showed decreased dystrophin expression and increased phosphorylated Smad levels compared with muscles injected with non-treated BMSCs. The opposite effects were seen after pretreatment with noggin, as expected.ConclusionsOur results identified BMP/Smad signaling as an essential negative regulator of promyogenic BMSC activity; inhibition of this pathway improved the efficiency of BMSC myogenic differentiation, which suggests that this pathway might serve as a target to regulate BMSC function for better myogenic differentiation during treatment of DMD and degenerative skeletal muscle diseases

Co-seismic signatures in magnetometer, geophone, and infrasound data during the Meinong Earthquake

This paper utilizes 10 stations of co-located seismometer, QuakeFinder/infrasound to observe co-seismic signatures triggered by the 6 February 2016 M 6.6 Meinong Earthquake. Each QuakeFinder system consists of a 3-axes induction magnetometer, an air conductivity sensor, a geophone, and temperature/relative humidity sensors. There are no obvious charges in the positive/negative ions, the temperature, and the humidity, while the magnetometer, the geophone, and infrasound data detect clear co-seismic signatures, similar to seismic waves recorded by seismometers. The magnetometers register high-frequency pulsations, like seismic waves, and superimpose with low-frequency variations, which could be caused by the magnetometer shaking/tilting and/or the underground water level change, respectively, upon the arrival of seismic waves. The spectrum centering around 2.0 Hz of the co-seismic geophone fluctuations is similar to that of the seismic waves. However, the energy of co-seismic geophone fluctuations (also magnetometer pulsations) yields an exponential decay to the distance of a station to the epicenter, while the energy of the seismic waves is inversely proportional to the square of the distance. This suggests that the mechanisms for detecting seismic waves of the QuakeFinder system and seismometers are different. In general, the geophone and magnetometer/infrasound system are useful to record high- and low-frequency seismic waves, respectively

The co-benefits of clean air and low-carbon policies on heavy metal emission reductions from coal-fired power plants in china

China has implemented a series of measures to address air pollutants and carbon emissions from coal-fired power plants, which can mitigate toxic heavy metal emissions simultaneously. By integrating plant-level information and energy activity data, we investigated the co-benefits of clean air and low-carbon policies by compiling a detailed inventory of historical heavy mental emissions (i.e., Hg, Pb, Cd, Cr, Ni, Sb, Mn, Co, Cu, Zn, As, and Se) for China's coal-fired power plants during 2005–2020. Several scenarios were then designed to assess the evolution of heavy metal emissions for each coal-fired power plant with consideration given to the coal washing rate, air pollution control devices, operational hours and lifetime. The total emissions decreased from 12.9 thousand tons in 2005 to 8.8 thousand tons in 2020, which was mainly due to the widely installation of upgraded end-of-pipe devices and the decommissioning of small and emission-intensive plants, especially in Sichuan, Jiangsu and Zhejiang. Scenario analysis shows that reducing the operational lifetime to 20 years is the most effective measure to reduce national HM emissions, but the effects differ widely between regions. This study provides insights for the precise co-control of both heavy metals and carbon emissions, which is highly important for meeting the requirements of the Minamata Convention and carbon neutrality

Changes of predominant species/biovars and sequence types of Brucellaisolates, Inner Mongolia, China

BACKGROUND: Human brucellosis incidence in China was divided into 3 stages, high incidence (1950-1960s), decline (1970-1980s) and re-emergence (1990-2000s). Human brucellosis has been reported in all the 32 provinces, of which Inner Mongolia has the highest prevalence, accounting for over 40% of the cases in China. To investigate the etiology alteration of human brucellosis in Inner Mongolia, the species, biovars and genotypes of 60 Brucella isolates from this province were analyzed. METHODS: Species and biovars of the Brucella strains isolated from outbreaks were determined based on classical identification procedures. Strains were genotyped by multi locus sequence typing (MLST). Sequences of 9 housekeeping genes were obtained and sequence types were defined. The distribution of species, biovars and sequence types (STs) among the three incidence stages were analyzed and compared. RESULTS: The three stages of high incidence, decline and re-emergence were predominated by B. melitensis biovar 2 and 3, B. abortus biovar 3, and B. melitensis biovar 1, respectively, implying changes in the predominant biovars. Genotyping by MLST revealed a total of 14 STs. Nine STs (from ST28 to ST36), accounting for 64.3% of all the STs, were newly defined and different from those observed in other countries. Different STs were distributed among the three stages. ST8 was the most common ST in 1950-1960s and 1990-2000s, while ST2 was the most common in 1970-1980s. CONCLUSIONS: The prevalence of biovars and sequence types of Brucella strains from Inner Mongolia has changed over time in the three stages. Compared with those from other countries, new sequence types of Brucella strains exist in China