5 research outputs found
DataSheet1_Genome-wide identification of dysregulated alternative splicing and RNA-binding proteins involved in atopic dermatitis.PDF
Objectives: We explored the role and molecular mechanisms of RNA-binding proteins (RBPs) and their regulated alternative splicing events (RASEs) in the pathogenesis of atopic dermatitis (AD).Methods: We downloaded RNA-seq data (GSE121212) from 10 healthy control skin samples (healthy, Ctrl), 10 non-lesional skin samples with AD damage (non-lesional, NL), and 10 lesional skin samples with AD damage (lesional, LS). We performed the analysis of differentially expressed genes (DEGs), differentially expressed RBPs (DE-RBPs), alternative splicing (AS), functional enrichment, the co-expression of RBPs and RASEs, and quantitative polymerase chain reaction (qPCR).Results: We identified 60 DE-RBP genes by intersecting 2141 RBP genes from existing reports with overall 2697 DEGs. Most of the DE-RBP genes were found to be upregulated in the AD LS group and related to immune and apoptosis pathways. We observed different ASEs and RASEs among the healthy, AD NL, and AD LS groups. In particular, alt3p and alt5p were the main ASEs and RASEs in AD NL and AD LS groups, compared to the healthy group. Furthermore, we constructed co-expression networks of DE-RBPs and RAS, with particular enrichment in biological pathways including cytoskeleton organization, inflammation, and immunity. Subsequently, we selected seven genes that are commonly present in these three pathways to assess their expression levels in the peripheral blood mononuclear cells (PBMCs) from both healthy individuals and AD patients. The results demonstrated the upregulation of four genes (IFI16, S100A9, PKM, and ENO1) in the PBMCs of AD patients, which is highly consistent with DE-RBP genes analysis. Finally, we selected four RAS genes regulated by RBPs that were related to immune pathways and examined their RASEs in PBMCs from both AD patients and healthy controls. The results revealed an increased percentage of RASEs in the DDX60 gene in AD, which is highly consistent with AS analysis.Conclusion: Dysregulated RBPs and their associated RASEs may have a significant regulatory role in the development of AD and could be potential therapeutic targets in the future.</p
Construction of Monoatomic-Modified Defective Ti<sup>4+</sup><sub>Ī±</sub>Ti<sup>3+</sup><sub>1āĪ±</sub>O<sub>2āĪ“</sub> Nanofibers for Photocatalytic Oxidation of HMF to Valuable Chemicals
Efficiently upgrading 5-hydroxymethylfurfural
(HMF) into
high-value-added
products, such as 2,5-diformylfuran (DFF) and 2,5-furan dicarboxylic
acid (FDCA), through a photocatalytic process by using solar energy
has been incessantly pursued worldwide. Herein, a series of transition-metal
(TM = Ni, Fe, Co, Cu) single atoms were supported on Ti4+Ī±Ti3+1āĪ±O2āĪ“ nanofibers (NFs) with certain defects (Ov),
denoted as TM SAC-Ti4+Ī±Ti3+1āĪ±O2āĪ“ NFs (TM =
Ni, Fe, Co, Cu), aiming to enhance the photocatalytic conversion of
HMF. A super HMF conversion rate of 57% and a total yield of 1718.66
Ī¼mol gā1 hā1 (DFF and FDCA)
surpassing that of the Ti4+Ī±Ti3+1āĪ±O2āĪ“ NFs by 1.6
and 2.1 times, respectively, are realized when TM is Co (Co SAC-Ti4+Ī±Ti3+1āĪ±O2āĪ“ NFs). Experiments combined with density
functional theory calculation (DFT) demonstrate that the TM single
atoms occupy the Ti site of Ti4+Ī±Ti3+1āĪ±O2āĪ“ NFs,
which plays a dominant role in the photo-oxidation of HMF. Raman,
X-ray photoelectron spectroscopy (XPS), and electron paramagnetic
resonance (EPR) characterizations confirm the strong electron local
exchange interaction in TM SAC-Ti4+Ī±Ti3+1āĪ±O2āĪ“ NFs
and demonstrate the substitution of Ti by the TM SACs. The projected
density of states and charge density difference reveal that the strong
interaction between metal-3d and O-2p orbitals forms TiāOāTM
bonds. The bonds are identified as the adsorption site, where TM single
atoms on the surface of Ti4+Ī±Ti3+1āĪ±O2āĪ“ NFs reduce
HMF molecule adsorption energy (Eads).
Furthermore, the TM single atom modulates the electronic structure
of TM SAC-Ti4+Ī±Ti3+1āĪ±O2āĪ“ NFs through electron transfer, leading
to narrow band gaps of the photocatalysts and enhancing their photocatalytic
performance. This study has uncovered a newer strategy for enhancing
the photocatalytic attributes of semiconducting materials
Gradient Structure Design of Flexible Waterborne Polyurethane Conductive Films for Ultraefficient Electromagnetic Shielding with Low Reflection Characteristic
Highly efficient
electromagnetic shielding materials entailing
strong electromagnetic wave absorption and low reflection have become
an increasing requirement for next-generation communication technologies
and high-power electronic instruments. In this study, a new strategy
is employed to provide flexible waterborne polyurethane composite
films with an ultra-efficient electromagnetic shielding effectiveness
(EMI SE) and low reflection by constructing gradient shielding layers
with a magnetic ferro/ferric oxide deposited on reduced graphene oxide
(rGO@Fe<sub>3</sub>O<sub>4</sub>) and silver-coated tetraneedle-like
ZnO whisker (T-ZnO/Ag) functional nanoparticles. Because of the differences
in density between rGO@Fe<sub>3</sub>O<sub>4</sub> and T-ZnO/Ag, a
gradient structure is automatically formed during the film formation
process. The gradient distribution of rGO@Fe<sub>3</sub>O<sub>4</sub> over the whole thickness range forms an efficient electromagnetic
wave absorption network that endows the film with a strong absorption
ability on the top side, while a thin layer of high-density T-ZnO/Ag
at the bottom constructs a highly conductive network that provides
an excellent electromagnetic reflection ability for the film. This
specific structure results in an āabsorbāreflectāreabsorbā
process when electromagnetic waves penetrate into the composite film,
leading to an excellent EMI shielding performance with an extremely
low reflection characteristic at a very low nanofiller content (0.8
vol % Fe<sub>3</sub>O<sub>4</sub>@rGO and 5.7 vol % T-ZnO/Ag): the
EMI SE reaches 87.2 dB against the X band with a thickness of only
0.5 mm, while the shielding effectiveness of reflection (SE<sub>R</sub>) is only 2.4 dB and the power coefficient of reflectivity (<i>R</i>) is as low as 0.39. This result means that only 39% of
the microwaves are reflected in the propagation process when 99.9999998%
are attenuated, which is the lowest value among the reported references.
This composite film with remarkable performance is suitable for application
in portable and wearable smart electronics, and this method offers
an effective strategy for absorption-dominated EMI shielding
PāDoped NiMn<sub>2</sub>O<sub>4</sub> Hollow Tubular Nanofiber Spinel Composites for Electrocatalytic Dechlorination
Electrochemical
degradation of dichloromethane (DCM) to produce
chloromethane is a hopeful strategy for the remediation of chlorinated
volatile organic compounds. However, developing high-efficiency electrocatalysts
without a noble metal remains a challenge. Here, we successfully constructed
P-doped NiMn2O4 hollow tubular nanofibers (Px-NM-HTNFs) by the combination of an electrostatic
spinning technique and a chemical vapor deposition technique, which
were used as efficient dechlorination electrocatalysts. Physicochemical
characterization and in situ characterization demonstrated that the
unique hollow tubular nanostructures and P-doped structure can effectively
accelerate charge transfer, expose more active sites, and optimize
the adsorption capacity of the electrocatalyst for DCM. The experimental
tests revealed that the P-doped electrocatalysts exhibited a remarkable
electrocatalytic dechlorination performance, achieving a chloromethane
production rate of 11665.46 Ī¼mol gā1 hā1 at ā3.03 V (vs Ag/AgCl/Me4NCl)
for P1.0-NM-HTNFs. In addition, the transfer coefficient
Ī± of 0.3 proved that the electrochemical degradation of DCM
conforms to the mechanism of synergistic dissociation electron transfer.
P1.0-NM-HTNFs generate the adsorption atom H*, thus facilitating
the dechlorination reaction. This work provides an idea for constructing
manganeseāspinel composite catalysts and producing chloromethane
with high value added for DCM electrochemical dechlorination
Synthesis and Identification of New Flavonoids Targeting Liver X Receptor Ī² Involved Pathway as Potential Facilitators of AĪ² Clearance with Reduced Lipid Accumulation
Alzheimerās
disease (AD) is associated with impaired AĪ²
degradation in the brain. Enhancing the process of AĪ² clearance
is an attractive potential AD therapy. Treatment with LXR agonists
may reduce AĪ² levels in vivo. However, the clinical potential
of many LXR agonists is limited because of their nonselective actions
on LXRĪ±/Ī², which lead to undesired hepatic lipogenesis
via LXRĪ±-dependent pathways. In this study, ABCA1 up-regulators
were identified from a series of flavonoids and were found to preferentially
activate LXRĪ² and up-regulate expression of ABCA1 and apoE in
different cell lines. Further investigations confirmed that these
compounds facilitate intracellular AĪ² clearance in AĪ²-loaded
BV2 cells. Administration of compound <b>19</b> reduced total
brain AĪ² and plaque burden in APP/PS1 double transgenic mice,
associated with elevated ABCA1 and apoE expression. Compared with
the nonselective LXR agonists, the active compounds reported here
induced less accumulation of undesired lipids and triglycerides in
HepG2 cells