Viral sequences obtained from public databases and used in the study.

a<p>The CP (AlsVX) or partial NP gene sequence (WBYVV) was determined in this study by amplification of the viral genomic region using RT-PCR and sequencing of the products.</p>b<p>Established and putative members of the genus.</p

Phylogenetic analysis of partial putative nucleocapsid protein (NP) gene sequences (518 nt; RNA3) of established and putative members of genus <i>Emaravirus</i>.

<p>The NP gene sequence of <i>Pigeonpea sterility mosaic virus</i> is not available and could not be included. Bootstrap values higher than 80 (of 100 replicates) are indicated on the branches. For accession numbers, see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0042758#pone-0042758-t001" target="_blank">Table 1</a>. Bar indicates 0.1 Kimura units <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0042758#pone.0042758-Kimura1" target="_blank">[34]</a>.</p

Percentage nucleotide (upper triangle) and deduced amino acid identities (lower triangle) of the coat protein genes of three isolates of <i>Alstroemeria virus X</i> and other potexviruses (see Table 1 for sequence accession numbers).

<p>Percentage nucleotide (upper triangle) and deduced amino acid identities (lower triangle) of the coat protein genes of three isolates of <i>Alstroemeria virus X</i> and other potexviruses (see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0042758#pone-0042758-t001" target="_blank">Table 1</a> for sequence accession numbers).</p

Percentage nucleotide (518 nt; upper triangle) and deduced amino acid identities (lower triangle) of the nucleocapsid protein gene of WBYVV and related negative-strand ssRNA viruses (see Table 1 for sequence accession numbers).

<p>Percentage nucleotide (518 nt; upper triangle) and deduced amino acid identities (lower triangle) of the nucleocapsid protein gene of WBYVV and related negative-strand ssRNA viruses (see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0042758#pone-0042758-t001" target="_blank">Table 1</a> for sequence accession numbers).</p

Leaves of woolly burdock (<i>A. tomentosum</i>) plants with and without virus-like symptoms.

<p>Plants Vi4, Vi5 and Vi8 (orange labels) were infected with WBYVV and the leaves display vein yellowing and mosaic symptoms. Plants were photographed in the Viikki Research Farm area on May 18, 2011.</p