Multifunctional Chitosan-45S5 Bioactive Glass-Poly(3-hydroxybutyrate-<i>co</i>-3-hydroxyvalerate) Microsphere Composite Membranes for Guided Tissue/Bone Regeneration

Novel multifunctional chitosan-45S5 bioactive glass-poly­(3-hydroxybutyrate-<i>co</i>-3-hydroxyvalerate) (PHBV) microsphere (CS-BG-MS) composite membranes were developed with applicability in guided tissue/bone regeneration (GTR/GBR). The incorporation of 45S5 BG and PHBV MS into CS membranes not only provided the membranes with favorable surface roughness, hydrophilicity, and flexibility but also slowed down their degradation rate. Moreover, the CS membranes became bioactive after the incorporation of 45S5 BG and capable of releasing drugs of different physicochemical properties in a controlled and sustained manner with the addition of PHBV MS. Cell culture tests showed that osteoblast-like MG-63 human osteosarcoma cells had significantly higher adhesion, cell proliferation, and alkaline phosphatase (ALP) activity on CS-BG and CS-BG-MS membranes than on neat CS membranes. Therefore, the developed bioactive CS-BG-MS membranes with potential multidrug (e.g., antibacterial and antiosteoporosis drugs) delivery capability are promising candidate membranes for GTR/GBR applications

Additional file 6: of Unmet needs of activities of daily living among a community-based sample of disabled elderly people in Eastern China: a cross-sectional study

Unmet Needs Assessment. (DOCX 22 kb

Additional file 4: of Unmet needs of activities of daily living among a community-based sample of disabled elderly people in Eastern China: a cross-sectional study

Zarit Burden Interview. (DOCX 17 kb

Additional file 1: of Unmet needs of activities of daily living among a community-based sample of disabled elderly people in Eastern China: a cross-sectional study

Demographic Questionnaire. (DOCX 18 kb

Additional file 2: of Unmet needs of activities of daily living among a community-based sample of disabled elderly people in Eastern China: a cross-sectional study

Barthel Index. (DOC 36 kb

Additional file 5: of Unmet needs of activities of daily living among a community-based sample of disabled elderly people in Eastern China: a cross-sectional study

Family Caregiver Task Inventory. (DOCX 18 kb

<i>CF</i>_<i>2</i><i>–II</i> Alternative Splicing Isoform Regulates the Expression of Xenobiotic Tolerance-Related Cytochrome P450 <i>CYP6CY22</i> in <i>Aphis gossypii</i> Glover

The expression of P450 genes is regulated by trans-regulatory factors or cis-regulatory elements and influences how endogenous or xenobiotic substances are metabolized in an organism’s tissues. In this study, we showed that overexpression of the cytochrome P450 gene, CYP6CY22, led to resistance to cyantraniliprole in Aphis gossypii. The expression of CYP6CY22 increased in the midgut and remaining carcass of the CyR strain, and after repressing the expression of CYP6CY22, the mortality of cotton aphids increased 2.08-fold after exposure to cyantraniliprole. Drosophila ectopically expressing CYP6CY22 exhibited tolerance to cyantraniliprole and cross-tolerance to xanthotoxin, quercetin, 2-tridecanone, tannic acid, and nicotine. Moreover, transcription factor CF2–II (XM_027994540.2) is transcribed only as the splicing variant isoform CF2–II-AS, which was found to be 504 nucleotides shorter than CF2–II in A. gossypii. RNAi and yeast one-hybrid (Y1H) results indicated that CF2–II-AS positively regulates CYP6CY22 and binds to cis-acting element p (−851/-842) of CYP6CY22 to regulate its overexpression. The above results indicated that CYP6CY22 was regulated by the splicing isoform CF2-II-AS, which will help us further understand the mechanism of transcriptional adaption of cross-tolerance between synthetic insecticides and plant secondary metabolites mediated by P450s