1 research outputs found
Carbon Nanodots-Based Fluorescent Turn-On Sensor Array for Biothiols
Biothiols
play important roles in biological processes. In this
study, a novel sensor array-based method was proposed to detect and
differentiate biothiols. The sensor array was constructed using three
kinds of Ag<sup>+</sup>–sensitive carbon nanodots (CDs). The
CDs were synthesized with amino acids and urea as carbon sources via
a simple microwave method. Results revealed that Ag<sup>+</sup> can
bind with CDs and depress the fluorescence of CDs, while the subsequently
joined biothiols can take Ag<sup>+</sup> away from CDs and recover
the fluorescence of CDs. Due to the different binding ability between
Ag<sup>+</sup> and various CDs, as well as Ag<sup>+</sup> and various
biothiols, the CD–Ag<sup>+</sup> array exhibits a unique pattern
of fluorescence variations when interacting with six biothiol samples
(cysteamine, dithiothreitol, mercaptosuccinic acid, glutathione, mercaptoacetic
acid, and mercaptoethanol). Principal component analysis (PCA) was
applied to analyze the pattern and generate a clustering map for a
clearer identification of these biothiols. PCA can also be employed
to simplify the established three-sensor array into a two-sensor array.
Both the three- and two-sensor arrays can identify these biothiols
in a wide biothiol concentration range (>10 μM)