Benefits of the antioxidant and anti-inflammatory activity of etoricoxib in the prevention of ovarian ischemia/reperfusion injury induced experimentally in rats

Yilmaz, Ismayil/0000-0001-7894-3613WOS: 000337510400027PubMed: 24888933Aim This study is a biochemical investigation of the effect of etoricoxib, a selective cyclooxygenase (COX)-2 inhibitor, on ischemia/reperfusion (I/R) injury experimentally induced in rat ovaries. Methods Experimental animals were divided into four groups: (i) ovarian ischemia/reperfusion (IRG); (ii) 30mg/kg etoricoxib+ovarian ischemia/reperfusion (EIRG-30); (iii) 60mg/kg etoricoxib+ovarian ischemia/reperfusion (EIRG-60); and (iv) a sham operation (SG) control group. Results the results showed levels of malondialdehyde in the IRG, EIRG-30, EIRG-60 and SG group ovarian tissue of 20.2 +/- 3.4, 11.2 +/- 3.2, 5.5 +/- 1.9 and 3.8 +/- 1.5mol/g protein, respectively. Myeloperoxidase activity for these groups was 24.2 +/- 6.7, 13 +/- 2.4, 4 +/- 1.8 and 3.5 +/- 1.9U/g protein, and total glutathione levels were 1.6 +/- 0.8, 4.5 +/- 1.9, 6.5 +/- 1.9 and 7.5 +/- 2.4nmol/g protein, respectively. COX-1 activity in IRG, EIRG-30, EIRG-60 and SG group rat ovarian tissue was 5.0 +/- 2.8, 12.2 +/- 2.4, 16.7 +/- 2.8 and 17.5 +/- 4.7U/mg protein, and COX-2 activity was 18.3 +/- 2.7, 3.5 +/- 1, 1.8 +/- 0.7 and 0.7 +/- 0.3U/mg protein, respectively. Conclusion Etoricoxib prevented oxidative damage induced with I/R in rat ovarian tissue. This property of etoricoxib suggests that it can be clinically beneficial in the prevention of damage that may arise with reperfusion by detorsion for the protection of the ovaries against torsion

Investigation and Histopathological Evaluation of the Effects of Omeprazole on the Ischemia-Reperfusion Induced Oxidative Damage and DNA Mutation in Rat Ovarian Tissue

In this study, the biochemical and histopathological effects of omeprazole on ischemia-reperfusion (I/R) induced oxidative damage in rat ovarian tissue were investigated. The moment that the animals remained motionless in supine position was considered the appropriate time to perform surgery. The ovaries of the rats were reached through a 2.0-2.5 cm long vertical incision in the lower abdomen. Subsequently, in the omeprazole (OIR) and the control groups (I/RC), a vascular clip was placed in the lower part of the right ovary (the part where the ovary is attached to the uterus) and ischemia was maintained for 3 h. (No ischemia was applied in the healthy group.) After this period, the vascular clip was removed in order to provide reperfusion for 2 h. Afterwards, all the animals were terminated by high dose-anesthesia, the ovaries were removed and histopathological and biochemical studies were performed. Omeprazole has an antioxidant effect and it can have a protective function in the oxidative damage induced by ischemia-reperfusion. We have found that omeprazole prevents oxidative damage due to ischemia-reperfusion injury in rat ovarian tissue

The impaired balances of oxidant/antioxidant and COX-1/COX-2 in ovarian ischemia-reperfusion injury and prevention by Nimesulide

Cetin, Nihal/0000-0003-3233-8009; Gul, Mehmet Ali/0000-0002-5849-0116;WOS: 000321086300017The aims of this study were to investigate the association of ovarian I/R injury with oxidant/antioxidant and cyclooxygenase activity and to examine the effect of nimesulide in I/R injury. Rats were divided into four groups: sham group, ischemia-reperfusion group (IR), nimesulide 25 mg/kg group (NIM 25), and nimesulide 50 mg/kg group (NIM 50). the severe oxidative stress and inflammation that occurred in the ovarian tissue treated I/R were recovered by treatment of nimesulide. the histopathological findings, severe haemorrhage, oedema, vascular congestion accompanied with migration and adhesion of polimorphonuclear leukocytes in the endothelium were observed in the IR group that MDA, MPO and COX-2 levels were found high whereas GSH and COX-1 levels were found low. the severe histopathological findings in IR group were moderate in NIM-25 group whereas those were slight in NIM-50 group. This finding suggests that nimesulide prevents injury due to reperfusion following ischemia better when used with dosage 50 mg/kg