The principle for design of the amplification primers.

<p>Multiplex PCR is performed as described previously [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0169219#pone.0169219.ref027" target="_blank">27</a>, <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0169219#pone.0169219.ref028" target="_blank">28</a>, <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0169219#pone.0169219.ref029" target="_blank">29</a>]. Asymmetric PCR procedure was used for generation of labeled single-stranded DNA in excess for hybridization and detection.</p

Mutations detected in the deaf individuals.

<p>Mutations detected in the deaf individuals.</p

Microarray design.

<p>(A) Copy of the microarray; (B) the microarray layout. QC: chemical control; PC: external control; BC: blank control; NC: negative control; IC: internal control; MC: magnetic control; W: wild-type; M: mutant.</p