Significance of the entire C-terminus in biological activities mediated by the RON receptor tyrosine kinase and its oncogenic variant RON160

The RON receptor tyrosine kinase regulates epithelial cell homeostasis and tumorigenesis by transducing multiple signals through its functional domains. The present study was to determine the significance of the entire C-terminus in RON or its variant RON160-mediated activities related to cell motility and tumorigenesis. Analysis of protein phosphorylation revealed that elimination of the entire C-terminus significantly impairs the ligand-dependent or independent RON or RON160 phosphorylation and dimerization. Phosphorylation of downstream signaling proteins such as Erk1/2, AKT, and p38 MAP kinase was also diminished in cells expressing the C-terminus-free RON or RON160. These dysfunctional activities were accompanied with the inability of truncated RON or RON160 to mediate cytoplasmic β-catenin accumulation. Functional analysis further demonstrated that truncation of the C-terminus significantly impairs RON or RON160-mediated cell proliferation, morphological changes, and cellular migration. Significantly, oncogenic RON160-mediated tumor growth in athymic nude mice was lost after the deletion of the C-terminus. Thus, the C-terminus is a critical component of the RON receptor. The entire C-terminus is required for RON or RON160-mediated intracellular signaling events leading to various cellular activities

Thin-layer cyclic voltammetric studies electron transfer across liquid/liquid interface

Thin layer cycle voltammetry is one of the most efficient methods to investigate liquid/liquid interface. The advantages of this approach lie on its simplicity and effectiveness. The paper represents the progress that has been made in experiments and theories, analyzing the factors that affect rate constants and discussing how to get steady-state currents

Multiscale bilateral filtering for improving image quality in digital breast tomosynthesis

Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/135115/1/mp3283.pd

Evaluation of the accuracy of SRTM3 and ASTER GDEM in the Tibetan Plateau mountain ranges

Topographic data on The Tibetan Plateau (TP) terrain are fundamental for geoscientific research, but are difficult to obtain. The Shuttle Radar Topographic Mission (SRTM) Digital Elevation Model (DEM) and the Advanced Spaceborne Thermal Emission and Reflection Radiometer Global Digital Elevation Model (ASTER GDEM) are two commonly used GDEM data. Verifying the accuracy of the two dataset for the TP mountain areas provides a reference point for the application of both DEMs. For evaluating the elevation accuracy and topographic information, we used 8242 field measurements from Differential Global Positioning System (DGPS) points and DEM data generated from 1:100,000 topographic maps to examine the accuracy of ASTER GDEM V2 and SRTM3 V4.1 elevation results. The average RMSE for elevation differences between DGPS and ASTER GDEM across the study areas was 18.56m while the average RMSE between DGPS and SRTM3 was 10.39m. The average RMSEs of ASTER GDEM and SRTM3 in glaciated areas were 8.55m and 5.87m, respectively. The vertical accuracy of SRTM3 is better than that of ASTER GDEM. The vertical accuracy of both DEMs do not vary with altitude, but is related to aspect and slope

Experimental Test of Bell inequalities with Six-Qubit Graph States

We report on the experimental realization of two different Bell inequality tests based on six-qubit linear-type and Y-shape graph states. For each of these states, the Bell inequalities tested are optimal in the sense that they provide the maximum violation among all Bell inequalities with stabilizing observables and possess the maximum resistance to noise.Comment: 4 pages, 2 figure

3D computational modeling and perceptual analysis of kinetic depth effects

Humans have the ability to perceive kinetic depth effects, i.e., to perceived 3D shapes from 2D projections of rotating 3D objects. This process is based on a variety of visual cues such as lighting and shading effects. However, when such cues are weak or missing, perception can become faulty, as demonstrated by the famous silhouette illusion example of the spinning dancer. Inspired by this, we establish objective and subjective evaluation models of rotated 3D objects by taking their projected 2D images as input. We investigate five different cues: ambient luminance, shading, rotation speed, perspective, and color difference between the objects and background. In the objective evaluation model, we first apply 3D reconstruction algorithms to obtain an objective reconstruction quality metric, and then use quadratic stepwise regression analysis to determine weights of depth cues to represent the reconstruction quality. In the subjective evaluation model, we use a comprehensive user study to reveal correlations with reaction time and accuracy, rotation speed, and perspective. The two evaluation models are generally consistent, and potentially of benefit to inter-disciplinary research into visual perception and 3D reconstruction