Research and application of key technology of intelligent coal caving in high gas fully-mechanized top coal caving face

At present, intelligent coal caving in high gas fully mechanized top coal caving face is facing many problems, mainly including poor recognition accuracy of coal gangue, incomplete control research on coal flow and gas concentration, cumbersome design and development of follow-up process, and slow response of control system.In order to solve these problems, this paper developed a cloud side collaborative intelligent coal caving control system based on 5G communication.The system includes 5G network, cloud server, edge processor, terminal execution equipment, etc.It has a high data transmission speed, control response speed and data processing capability.Based on the analysis of fully mechanized coal caving process parameters and intelligent coal caving process flow, elaborates the four key technologies of intelligent coal caving the coal gangue identification technology, big block coal identification and coal flow load balance technology, gas safety linkage control technology, and digital twin technology of coal caving following machine.The application test was carried out in Baode 81309 fully mechanized top coal caving face.After the intelligent fully mechanized top coal caving technology was adopted, the top coal recovery rate increased from 86% to 93%, the coal gangue rate decreased from 21% to 15%, the total production efficiency increased by 10%, and the number of coal caving operators decreased from 3 to 4 to 1 to 2.The cloud edge collaborative intelligent coal caving control system based on 5G communication developed in this paper and its key technologies can also be extended to other fully mechanized coal caving faces, which is of great value for reducing personnel and increasing efficiency and safe and efficient mining of coal mines

Gujin Dan is a Chinese medicine formulation that stimulates cell proliferation and differentiation by controlling multiple genes involved in MC3T3-E1 cells

Background: With the development of Traditional Chinese medicine (TCM) in recent years, the use of TCM in the treatment of osteoporosis has received much attention and research. Gujin Dan (GJD) is one of the representative Chinese medicine formulations that work synergistically with 19 herbs and has been used for decades to treat cervical spondylosis, lumbar disc herniation, osteoarthritis and osteoporosis. However, the exact molecular mechanism by which GJD is used to strengthen bones in the treatment of osteoporosis remains largely unknown. / Methods: In this study, an aqueous extract of GJD was prepared and its components were identified by high-performance liquid chromatography (HPLC). The effect of GJD aqueous extract on MC3T3-E1 cells was determined by Cell Counting Kit-8 (CCK-8) assay, alkaline phosphatase (ALP), and alizarin red S staining (ARS), combined with RNA sequencing (RNA-seq) and qRT-PCR. / Results: Our study showed that GJD significantly promoted the proliferation of MC3T3-E1 cells, as well as the synthesis and mineralisation of the extracellular matrix. GJD significantly increased the expression levels of genes that promote cell proliferation such as Adamts1, Mcam, Cyr61, Fos, Cebpd, Fosl2, Sirt1, Nipbl, Sema3c and Kcnq1ot1, up-regulated genes that inhibit apoptosis such as Gadd45a, Birc3, up-regulated genes that inhibit osteoclastogenesis such as Bcl6, Nfkbiz, Clcf1, Bcl3, Lgals3, Wisp1, Dusp1 and Fblim1, up-regulated genes that promote MC3T3-E1 cell differentiation such as Junb, Egr1, Klf10, Atf6, Malat1, Btg2, Sertad4, Zfyve16, Tet2, Creb5, Snai2, Fam46a, Calcrl and Pdzrn3. In addition, GJD mildly upregulated the expression levels of gene markers such as Atf4, Fn1, Usp7, Sox4, Col16a1, Spp1, Bmp1, Runx2, Bglap, Col12a1, and Alpl in osteoblasts. / Conclusions: Our results show that GJD promotes the differentiation and proliferation of MC3T3-E1 cells, inhibits osteoclast formation, and prevents osteoblast apoptosis. The present study significantly improves the current understanding of the molecular effects of GJD on MC3T3-E1 cells. This study also provides a new strategy for the further use of Chinese medicinal preparations against bone metabolism-related diseases

Observation of Fungi, Bacteria, and Parasites in Clinical Skin Samples Using Scanning Electron Microscopy

This chapter highlights the description of the clinical manifestation and its pathogen and the host tissue damage observed under the Scanning Electron Microscope, which helps the clinician to understand the pathogen’s superstructure, the change of host subcell structure, and the laboratory workers to understand the clinical characteristics of pathogen-induced human skin lesions, to establish a two-way learning exchange database with vivid image

GJB2 mutation spectrum in 2063 Chinese patients with nonsyndromic hearing impairment

Background: Mutations in GJB2 are the most common molecular defects responsible for autosomal recessive nonsyndromic hearing impairment (NSHI). The mutation spectra of this gene vary among different ethnic groups. Methods: In order to understand the spectrum and frequency of GJB2 mutations in the Chinese population, the coding region of the GJB2 gene from 2063 unrelated patients with NSHI was PCR amplified and sequenced. Results: A total of 23 pathogenic mutations were identified. Among them, five (p.W3X, c.99delT, c.155_c.158delTCTG, c.512_c.513insAACG, and p.Y152X) are novel. Three hundred and seven patients carry two confirmed pathogenic mutations, including 178 homozygotes and 129 compound heterozygotes. One hundred twenty five patients carry only one mutant allele. Thus, GJB2 mutations account for 17.9% of the mutant alleles in 2063 NSHI patients. Overall, 92.6% (684/739) of the pathogenic mutations are frame-shift truncation or nonsense mutations. The four prevalent mutations; c.235delC, c.299_c.300delAT, c.176_c.191del16, and c.35delG, account for 88.0% of all mutantalleles identified. The frequency of GJB2 mutations (alleles) varies from 4% to 30.4% among different regions of China. It also varies among different sub-ethnic groups. Conclusion: In some regions of China, testing of the three most common mutations can identify at least one GJB2 mutant allele in all patients. In other regions such as Tibet, the three most common mutations account for only 16% the GJB2 mutant alleles. Thus, in this region, sequencing of GJB2 would be recommended. In addition, the etiology of more than 80% of the mutant alleles for NSHI in China remains to be identified. Analysis of other NSHI related genes will be necessary

Discovery of DNA Motif Utilising an Integrated Strategy Based on Random Projection and Particle Swarm Optimization

During the process of gene expression and regulation, the DNA genetic information can be transferred to protein by means of transcription. The recognition of transcription factor binding sites can help to understand the evolutionary relations among different sequences. Thus, the problem of recognition of transcription factor binding sites, i.e., motif recognition, plays an important role for understanding the biological functions or meanings of sequences. However, when the established search space processes much noise subsequences, many optimization algorithms tend to be trapped into local optimum. In order to solve this problem, a particle swarm optimization and random projection-based algorithm (PSORPS) is proposed for recognizing DNA motifs. First, a random projection strategy is employed to filter the noise subsequences for constructing the objective space. Moreover, the sequence segments distributed in the majority of DNA sequences can be obtained and used for the population initialization of PSO. Then, the motifs of DNA sequences can be automatically searched by using a designed PSO algorithm in the constructed l-mer objective space. Finally, to alleviate the base deviation and further improve the recognition accuracy, the two operators of associated drift and independent drift are performed on the optimization results obtained by PSO. The experiments are conducted on real-world biological datasets, and the experimental results verify the effectiveness of the proposed algorithm

Reusable and Dual-Potential Responses Electrogenerated Chemiluminescence Biosensor for Synchronously Cytosensing and Dynamic Cell Surface N‑Glycan Evaluation

A novel reusable and dual-potential responsive electrogenerated chemiluminescence (ECL) biosensor was fabricated for synchronous detection of cancer cells and their surface N-glycan. In this strategy, a cancer cell recognized aptamer hybridized with a capture DNA was immobilized on electrochemically reduced MoS₂ nanosheets, and Ru­(phen)₃²⁺ as ECL probes was intercalated into the grooves of the double-strand DNA. In the presence of target cells, the capture DNA and Ru­(phen)₃²⁺ were released from the electrode interface owing to the specific interaction between cancer cells and the aptamer. Meanwhile, concanavalin A (Con A), a mannose binding protein, and a conjugated gold nanoparticle modified graphite-C₃N₄ (Con A@Au-C₃N₄) was used as a negative ECL nanoprobe and applied for the cell surface N-glycan evaluation owing to the excellent ECL properties of g-C₃N₄ at negative potential. The cytosensing and cell surface N-glycan evaluation could be simultaneously realized with high sensitivity and excellent selectivity based on the ratio of ECL intensity between the negative potential and positive potential (ΔECL_n/ΔECL_p), avoiding the traditional routing cell counting procedures. Moreover, the aptamer modified electrode can be regenerated in the presence of capture DNA solutions for cyclic utilization. As a proof-of-concept, the ECL cytosensor showed excellent performances for the analysis of the MCF-7 cancer cell and its surface N-glycan evaluation in human serum samples. The reusable and dual potential response ECL biosensor endows a feasibility tool for clinical diagnosis and drug screening especially in complex biological systems

Hydrazine-Linked Convergent Self-Assembly of Sophisticated Concave Polyhedrons of beta-Ni(OH)(2) and NiO from Nanoplate Building Blocks

Intelligent self-assembly of nanobuilding blocks during the formation of Ni(OH)(2) single crystals with concave polyhedron structure was studied. The nickel hydroxide nanoplates pile up together with the aid of hydrazine molecules and grow along the [001] direction to form the brand-new geometry of concave polyhedrons. The novel nanostructure looks like a regularly twisted triangular prism wringed smoothly in the middle and could be attributed to the inherent spatial asymmetry of the nickel hydroxide crystal structure and the hydrogen bonds linked with the added hydrazine molecules. On the basis of the putative mechanism, polyhedrons with different longitudinal sizes (0.3-1 mu m) were synthesized by adjusting the quantities of hydrazine. We also obtained NiO polyhedron crystals with similarly piled plates by calcinations of the Ni(OH)(2) polyhedrons. Our preliminary electrochemical experiments show that the Ni(OH)(2) polyhedrons have potential applications in electrochemical storage