12 research outputs found
RNAi inhibits viral protein.
<p>RD cells transfected with siRNA were infected with strain EV71-2006-52-9 at an MOI of 0.01. At 36 h postinfection, total protein was extracted and analyzed with western blotting. β-Actin was used as the internal loading control. The protein measurements were made in three independent experiments.</p
Transfection efficiency of siRNA in RD cells.
<p>(A) Cellular distribution of BLOCK-iT Fluorescent Oligo in transfected RD cells. RD cells were transfected with different concentrations of BLOCK-iT Fluorescent Oligo and 2 μl of Lipofectamine 2000. At 24 h after transfection, the cells were observed under a fluorescence microscope. (B) RD cells transfected with BLOCK-iT Fluorescent Oligo were quantified with flow cytometry. The cells were assayed in three independent experiments.</p
RNAi inhibits viral RNA.
<p>RD cells were treated with each siRNA and then infected with strain EV71-2006-52-9 at an MOI of 0.01. At 24 h postinfection, the viral RNA was extracted and analyzed with real-time RT–PCR. The data shown represent the means ± SD of three independent experiments (**p < 0.01).</p
Sequences of the synthesized siRNA molecules and their positions in the EV71 2A<sup>pro</sup> genomic region.
<p>Sequences of the synthesized siRNA molecules and their positions in the EV71 2A<sup>pro</sup> genomic region.</p
siRNAs protect cells against EV71-induced cytopathic effects.
<p>Morphological changes in RD cells were observed after infection. Cells were transfected with each siRNA at a final concentration of 60 nM and then infected with strain EV71-2006-52-9 at an MOI of 0.01. Micrographs were taken at 48 h postinfection under an inverted microscope. The tests were performed in three independent experiments.</p
RNAi inhibits the replication of different EV71 strains.
<p>RD cells transfected with siRNA were infected with strain EV71-2008-43-16 at an MOI of 0.01. Total protein was extracted at 36 h after infection, and VP1 expression was analyzed with western blotting. β-Actin was used as the internal loading control. Protein measurements were made in three independent experiments.</p
Viral titer assay.
<p>Virus titers were determined in term of TCID<sub>50</sub>. RD cells pretreated with siRNAs were infected with strain EV71-2006-52-9 at an MOI of 0.01. At 48 h postinfection, the supernatants were collected to detect the progeny viral titers. Data are the means ± SD of three independent experiments (**p < 0.01).</p
Bis-tridentate Ir(III) Phosphors and Blue Hyperphosphorescence with Suppressed Efficiency Roll-Off at High Brightness
Narrowband blue emitters are indispensable in achieving
ultrahigh-definition
OLED displays that satisfy the stringent BT 2020 standard. Hereby,
a series of bis-tridentate Ir(III) complexes bearing electron-deficient
imidazo[4,5-b]pyridin-2-ylidene carbene coordination
fragments and 2,6-diaryloxy pyridine ancillary groups were designed
and synthesized. They exhibited deep blue emission with quantum yields
of up to 89% and a radiative lifetime of 0.71 μs in the DPEPO
host matrix, indicating both the high efficiency and excellent energy
transfer process from the host to dopant. The OLED based on Irtb1 showed an emission at 468 nm with a maximum external
quantum efficiency (EQE) of 22.7%. Moreover, the hyper-OLED with Irtb1 as a sensitizer for transferring energy to terminal
emitter v-DABNA exhibited a narrowband blue emission at 472 nm and
full width at half-maximum (FWHM) of 24 nm, a maximum EQE of 23.5%,
and EQEs of 19.7, 16.1, and 12.9% at a practical brightness of 100,
1000, and 5000 cd/m2, respectively
Distribution of the outbreak strains and onset time.
<p>Distribution of the outbreak strains and onset time.</p
Phylogenetic analysis of the six outbreak strains.
<p>A phylogenetic tree was constructed from multiple alignment of the core genome SNPs of the 6 isolates in the present study and another 4 previous isolates from GenBank, where FPR3757 USA300 was included as an outgroup. A phylogenetic tree with 1000 bootstrap resamples of the alignment data sets was generated using the neighbor-joining method in MEGA5.0 with the contribution model of "Kimura 2-parameter". Bootstrap values are indicated at the nodes. The scale bar indicates the number of substitutions per position for a unit branch length.</p