Functional comparison of plasma-membrane Na/Hantiporters from two pathogenic species-3

Phase and proteins extracted. After separation of proteins via SDS-PAGE they were transferred to nitrocellulose membrane and detected with anti-GFP antibody.<p><b>Copyright information:</b></p><p>Taken from "Functional comparison of plasma-membrane Na/Hantiporters from two pathogenic species"</p><p>http://www.biomedcentral.com/1471-2180/8/80</p><p>BMC Microbiology 2008;8():80-80.</p><p>Published online 20 May 2008</p><p>PMCID:PMC2424070.</p><p></p

Functional comparison of plasma-membrane Na/Hantiporters from two pathogenic species-1

At 30°C. Cells with empty YEp352 vector (-) were used as negative, cells expressing Nha1p as positive control, respectively. Pictures were taken after 4 (YNB) or 7 days (YNB + salts) of incubation.<p><b>Copyright information:</b></p><p>Taken from "Functional comparison of plasma-membrane Na/Hantiporters from two pathogenic species"</p><p>http://www.biomedcentral.com/1471-2180/8/80</p><p>BMC Microbiology 2008;8():80-80.</p><p>Published online 20 May 2008</p><p>PMCID:PMC2424070.</p><p></p

Functional comparison of plasma-membrane Na/Hantiporters from two pathogenic species-4

or expressing Cnh1p (■), Cnh1p (▲), Cnh1p (♦) antiporters was measured. The initial concentrations of cations in cells (corresponding to 100%) were as follows: Na, 110.5 ± 6.8 nmol (mg dry wt), K, 549.5 ± 25.7 nmol (mg dry wt)(representative results are shown).<p><b>Copyright information:</b></p><p>Taken from "Functional comparison of plasma-membrane Na/Hantiporters from two pathogenic species"</p><p>http://www.biomedcentral.com/1471-2180/8/80</p><p>BMC Microbiology 2008;8():80-80.</p><p>Published online 20 May 2008</p><p>PMCID:PMC2424070.</p><p></p

Growth of various species and on YPD medium supplemented with salts at 30°C

<p><b>Copyright information:</b></p><p>Taken from "Functional comparison of plasma-membrane Na/Hantiporters from two pathogenic species"</p><p>http://www.biomedcentral.com/1471-2180/8/80</p><p>BMC Microbiology 2008;8():80-80.</p><p>Published online 20 May 2008</p><p>PMCID:PMC2424070.</p><p></p

Fractionation of Soda Pulp Lignin in Aqueous Solvent through Membrane-Assisted Ultrafiltration

An industrial wheat straw lignin was fractionated by a multistep process involving microfiltration followed by two membrane-assisted ultrafiltration steps starting from an aqueous solvent solution. The parent lignin and the different fractions were fully characterized in terms of chemical composition and physicochemical properties by gel permeation chromatography, gas chromatography–mass spectrometry, high-performance liquid chromatography, thermogravimetric analysis, differential scanning calorimetry analysis, and Fourier transform infrared spectroscopy. The results show that the proposed process allows us to selectively control the molar mass distribution of the fractions and the related dependent properties. This strategy offers a better understanding of the structural complexity of soda pulp raw lignin and emerges as an essential tool for lignin valorization in the context of material science and preparative organic chemistry