Aedes aegypti CLIPB9 activates prophenoloxidase-3 in the presence of CLIPA14 after fungal infection

Melanization is an integral part of the insect defense system and is often induced by pathogen invasion. Phenoloxidases (POs) are critical enzymes that catalyze melanin formation. PO3 is associated with the antifungal response of the mosquito, Aedes aegypti, but the molecular mechanism of the prophenoloxidase-3 (PPO3) activation is unclear. Here we report that PPO3 cleavage activation is mediated by a clip-domain serine protease, CLIPB9. We purified recombinant CLIPB9 and found that it cleaved PPO3 and increased PO activity in the hemolymph. We then identified CLIPA14 (a serine protease homolog) by co-immunoprecipitation using anti-CLIPB9 antibody. After being cleaved by CLIPB9, Ae. aegypti CLIPA14 acted as a cofactor for PPO3 activation. In addition, dsRNA co-silencing of CLIPB9 and CLIPA14 genes reduced melanization after infection with the entomopathogen, Beauveria bassiana, making the adult mosquitoes more sensitive to fungal infection. These results illustrate the roles of CLIPB9 and CLIPA14 in the PPO activation pathway and revealed the complexity of the upstream serine protease network controlling melanization

Biological and Psychological Perspectives of Resilience: Is It Possible to Improve Stress Resistance?

The term “resilience” refers to the ability to adapt successfully to stress, trauma and adversity, enabling individuals to avoid stress-induced mental disorders such as depression, posttraumatic stress disorder (PTSD) and anxiety. Here, we review evidence from both animal models and humans that is increasingly revealing the neurophysiological and neuropsychological mechanisms that underlie stress susceptibility, as well as active mechanisms underlying the resilience phenotype. Ultimately, this growing understanding of the neurobiological mechanisms of resilience should result in the development of novel interventions that specifically target neural circuitry and brain areas that enhance resilience and lead to more effective treatments for stress-induced disorders. Stress resilience can be improved, but the outcomes and effects depend on the type of intervention and the species treated

Optical Temperature Sensing of YbNbO4:Er3+ Phosphors Synthesized by Hydrothermal Method

The novel YbNbO4:Er3+ phosphors were firstly synthesized through the hydrothermal method by adding LiOH·H2O as flux in the H2O/EG system. YbNbO4:Er3+ phosphors showed the agglomerated irregular polygons coexisting with some tiny grains. XRD and Raman spectra were measured to understand the phase structure and the crystal growth mechanism of YbNbO4:Er3+ phosphors. The upconversion (UC) emission spectra, the pump power dependency and UC mechanism were studied under 980 nm excitation. Based on the fluorescence intensity ratio technique, YbNbO4:Er3+ exhibited the maximum sensor sensitivity of 0.00712 K−1 at 220 K, providing a promising application in optical low-temperature sensors

Impacts of deep-rooted fruit trees on recharge of deep soil water using stable and radioactive isotopes

Deep-rooted fruit trees mine more water from deep soils than their shallow-rooted counterparts. Understanding how deep soil water (DSW) is replenished and subsequently depleted by deep-rooted fruit trees, therefore, are important for informing sustainable water resources management particularly in arid regions. In this study, we collected soil samples from the surface down to 20 m under four land use types (farmland, 8-year apple orchard, 12-year peach orchard, and 25-year apple orchard) in China's Loess Plateau. We then measured the soil water content, stable (δ2H and δ18O) and radioactive (3H) isotopic compositions. The radioactive isotope was used to constrain the age of soil water while the stable isotopes were used to determine the types of storms that would have contributed to recharging the DSW. We then implemented a soil water balance model to identify the mechanisms underlying the changes in DSW. Mechanistically, our results show that water movement in these soils was predominantly via piston flow. The age of DSW below 8 m was determined to be older than 55 years. Altogether, these results support an interpretation that DSW may have only been recharged by high-intensity, low-frequency rainfall events during the wet season (July to September), but that the magnitude of DSW recharge was likely to be influenced by subsequent water mining by deep-rooted fruit trees. The deep-rooted fruit trees consumed more DSW than farmland vegetation, substantially limiting the magnitude of DSW recharge under the orchards. Our simple soil water balance model, informed by water stable isotopes and supplemented with information from tritium, provides a technique for partitioning soil water balance (SWB) and insights into the long-term effects of land use change on water resources in arid regions

Association between different combination of measures for obesity and new-onset gallstone disease.

Body mass index(BMI) is a calculation index of general obesity. Waist circumference(WC) is a measure of body-fat distribution and always used to estimate abdominal obesity. An important trait of general obesity and abdominal obesity is their propensity to coexist. Using one single measure of obesity could not estimate persons at risk for GSD precisely.This study aimed to compare the predictive values of various combination of measures for obesity(BMI, WC, waist to hip ratio) for new-onset GSD.We prospectively studied the predictive values of various combination of measures for obesity for new-onset GSD in a cohort of 88,947 participants who were free of prior gallstone disease, demographic characteristics and biochemical parameters were recorded.4,329 participants were identified to have GSD among 88,947 participants during 713 345 person-years of follow-up. Higher BMI, WC and waist to hip ratio (WHtR) were significantly associated with higher risks of GSD in both genders even after adjustment for potential confounders. In males, the hazard ratio for the highest versus lowest BMI, WC, WHtR were 1.63(1.47~1.79), 1.53(1.40~1.68), 1.44(1.31~1.58), respectively. In females, the hazard ratio for the highest versus lowest BMI, WC, WHtR were 2.11(1.79~2.49), 1.85(1.55~2.22), 1.84(1.55~2.19), respectively. In male group, the combination of BMI+WC improved the predictive ability of the model more clearly than other combinations after adding them to the multivariate model in turn, while for females the best predictive combination was BMI+WHtR.Elevated BMI, WC and WHtR were independent risk factors for new-onset GSD in both sex groups after additional adjustment was made for potential confounders. In males, the combination of BMI+WC seemed to be the most predictable model to evaluate the effect of obesity on new-onset GSD, while the best combination in females was BMI+WHtR

Interval Number-Based Safety Reasoning Method for Verification of Decentralized Power Systems in High-Speed Trains

Decentralized power systems are commonly used in high-speed trains. However, many parameters in decentralized power systems are uncertain and inevitably have errors. We present a reasoning method based on the interval numbers for decentralized power systems in high-speed trains. Uncertain parameters and their unavoidable errors are quantitatively described by interval numbers. We also define generalized linear equations with interval numbers (LAIs), which can be used to describe the movement of the train. Furthermore, it is proven that the zero sets of LAIs are convex. Therefore, the inside of the fault-tolerance area can be formed by their vertexes and edges and represented by linear inequalities. Consequently, we can judge whether the system is working properly by verifying that the current system state is in the fault-tolerance area. Finally, a fault-tolerance area is obtained, which can be determined by linear equations with an interval number, and we test the correctness of the fault-tolerance area through large-scale random test cases

Impacts of deep-rooted fruit trees on recharge of deep soil water using stable and radioactive isotopes

Deep-rooted fruit trees mine more water from deep soils than their shallow-rooted counterparts. Understanding how deep soil water (DSW) is replenished and subsequently depleted by deep-rooted fruit trees, therefore, are important for informing sustainable water resources management particularly in arid regions. In this study, we collected soil samples from the surface down to 20 m under four land use types (farmland, 8-year apple orchard, 12-year peach orchard, and 25-year apple orchard) in China's Loess Plateau. We then measured the soil water content, stable (δ2H and δ18O) and radioactive (3H) isotopic compositions. The radioactive isotope was used to constrain the age of soil water while the stable isotopes were used to determine the types of storms that would have contributed to recharging the DSW. We then implemented a soil water balance model to identify the mechanisms underlying the changes in DSW. Mechanistically, our results show that water movement in these soils was predominantly via piston flow. The age of DSW below 8 m was determined to be older than 55 years. Altogether, these results support an interpretation that DSW may have only been recharged by high-intensity, low-frequency rainfall events during the wet season (July to September), but that the magnitude of DSW recharge was likely to be influenced by subsequent water mining by deep-rooted fruit trees. The deep-rooted fruit trees consumed more DSW than farmland vegetation, substantially limiting the magnitude of DSW recharge under the orchards. Our simple soil water balance model, informed by water stable isotopes and supplemented with information from tritium, provides a technique for partitioning soil water balance (SWB) and insights into the long-term effects of land use change on water resources in arid regions