8 research outputs found

    Additional file 2: of Using proteomic analysis to investigate uniconazole-induced phytohormone variation and starch accumulation in duckweed (Landoltia punctata)

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    Down-regulated proteins after application of uniconazole. The down-regulated proteins with <0.8-fold change and p-value less than 0.05 and their ratios among all comparative groups listed in the table. (XLSX 46 kb

    Investigation of Charge-Ordered Barium Iron Fluorides with One-Dimensional Structural Diversity and Complex Magnetic Interactions

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    Three mixed-valence barium iron fluorides, Ba7Fe7F34, Ba2Fe2F9, and BaFe2F7, were prepared through hydrothermal redox reactions. The characteristic structures of these compounds feature diverse distributions of FeIIF6 octahedra and FeIIIF6 groups. Ba7Fe7F34 contained one-dimensional infinite ∞[FeIIFeIII6F34]14– double chains, comprising cis corner-sharing octahedra along the b direction; Ba2Fe2F9 contained one-dimensional ∞[Fe2F9]4– double chains, consisting of cis corner-sharing octahedra along the chain (a-axis direction) and trans corner-sharing octahedra vertical to the chain, while BaFe2F7 revealed three-dimensional (3D) frameworks that consist of isolated edge-sharing dinuclear FeII2F10 units linked via corners by FeIIIF6 octahedra. Magnetization and Mössbauer spectroscopy measurements revealed that Ba7Fe7F34 exhibits an antiferromagnetic phase transition at ∼11 K, where ferrimagnetic ∞[FeIIFeIII6F34]14– double chains are arranged in a paralleling manner, while Ba2Fe2F9 shows canted antiferromagnetic ordering at ∼32.5 K, leading to noncollinear spin ordering

    DataSheet_1_An endogenous promoter LpSUT2 discovered in duckweed: a promising transgenic tool for plants.zip

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    Promoters are one of the most critical elements in regulating gene expression. They are considered essential biotechnological tools for heterologous protein production. The one most widely used in plants is the 35S promoter from cauliflower mosaic virus. However, our study for the first time discovered the 35S promoter reduced the expression of exogenous proteins under increased antibiotic stress. We discovered an endogenous strong promoter from duckweed named LpSUT2 that keeps higher initiation activity under antibiotic stress. Stable transformation in duckweed showed that the gene expression of eGFP in the LpSUT2:eGFP was 1.76 times that of the 35S:eGFP at 100 mg.L-1 G418 and 6.18 times at 500 mg.L-1 G418. Notably, with the increase of G418 concentration, the gene expression and the fluorescence signal of eGFP in the 35S:eGFP were weakened, while the LpSUT2:eGFP only changed slightly. This is because, under high antibiotic stress, the 35S promoter was methylated, leading to the gene silencing of the eGFP gene. Meanwhile, the LpSUT2 promoter was not methylated and maintained high activity. This is a previously unknown mechanism that provides us with new insights into screening more stable promoters that are less affected by environmental stress. These outcomes suggest that the LpSUT2 promoter has a high capacity to initiate the expression of exogenous proteins. In conclusion, our study provides a promoter tool with potential application for plant genetic engineering and also provides new insights into screening promoters.</p

    Supplemental Material, Appendix_2 - Evaluating Completeness of Reporting in Behavioral Interventions Pilot Trials

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    <p>Supplemental Material, Appendix_2 for Evaluating Completeness of Reporting in Behavioral Interventions Pilot Trials by Meha Bhatt, Laura Zielinski, Nitika Sanger, Ieta Shams, Candice Luo, Bianca Bantoto, Hamnah Shahid, Guowei Li, Luciana P. F. Abbade, Ikunna Nwosu, Yanling Jin, Mei Wang, Yaping Chang, Guangwen Sun, Lawrence Mbuagbaw, Mitchell A. H. Levine, Jonathan D. Adachi, Lehana Thabane, and Zainab Samaan in Research on Social Work Practice</p

    Supplemental Material, Appendix_1 - Evaluating Completeness of Reporting in Behavioral Interventions Pilot Trials

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    <p>Supplemental Material, Appendix_1 for Evaluating Completeness of Reporting in Behavioral Interventions Pilot Trials by Meha Bhatt, Laura Zielinski, Nitika Sanger, Ieta Shams, Candice Luo, Bianca Bantoto, Hamnah Shahid, Guowei Li, Luciana P. F. Abbade, Ikunna Nwosu, Yanling Jin, Mei Wang, Yaping Chang, Guangwen Sun, Lawrence Mbuagbaw, Mitchell A. H. Levine, Jonathan D. Adachi, Lehana Thabane, and Zainab Samaan in Research on Social Work Practice</p

    Supplemental Material, Appendix_3 - Evaluating Completeness of Reporting in Behavioral Interventions Pilot Trials

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    <p>Supplemental Material, Appendix_3 for Evaluating Completeness of Reporting in Behavioral Interventions Pilot Trials by Meha Bhatt, Laura Zielinski, Nitika Sanger, Ieta Shams, Candice Luo, Bianca Bantoto, Hamnah Shahid, Guowei Li, Luciana P. F. Abbade, Ikunna Nwosu, Yanling Jin, Mei Wang, Yaping Chang, Guangwen Sun, Lawrence Mbuagbaw, Mitchell A. H. Levine, Jonathan D. Adachi, Lehana Thabane, and Zainab Samaan in Research on Social Work Practice</p

    Supplemental Material, Supplementary_Material - A Systematic Survey of Control Groups in Behavioral and Social Science Trials

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    <p>Supplemental Material, Supplementary_Material for A Systematic Survey of Control Groups in Behavioral and Social Science Trials by Mei Wang, Guangwen Sun, Yaping Chang, Yanling Jin, Alvin Leenus, Muhammad Maaz, Guowei Li, Meha Bhatt, Luciana P. F. Abbade, Ikunna Nwosu, Laura Zielinski, Nitika Sanger, Bianca Bantoto, Candice Luo, Ieta Shams, Hamnah Shahid, Jonathan Adachi, Lawrence Mbuagbaw, Mitchell Levine, Zainab Samaan, and Lehana Thabane in Research on Social Work Practice</p
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