33 research outputs found
Data_Sheet_1_Remote diffusion-weighted imaging lesions and blood pressure variability in primary intracerebral hemorrhage.docx
ObjectiveThe aim of this study was to examine the association between remote diffusion-weighted imaging lesions (R-DWILs) and blood pressure variability (BPV) in patients with primary intracerebral hemorrhage (ICH).MethodsWe conducted a retrospective review of a consecutive cohort of 375 patients with primary ICH within 24 h onset. R-DWILs were defined as hyperintensity lesions in DWI remote from the hematoma. Blood pressure recordings were extracted up to 24 h post-admission. BPV was measured using SD, coefficient of variation (CV), and successive variation (SV).ResultsRemote DWI lesions were detected in 65 (17.3%) primary ICH patients. In multivariable logistic regression analysis, parameters of BPV were independently associated with R-DWILs, and the results remained consistent after being adjusted with mean SBP. SD, CV, and SV values in the highest quintile, showed 3- to 8-fold increased risk of R-DWILs, compared with the lowest quintile. ΔSBP demonstrated a significant difference in 2 different predictive models. Max SBP only dictated a significant difference in model 1. Mean SBP, admission SBP, and min SBP, failed to present an association with R-DWILs in model 1 or model 2.ConclusionOur results provided additional evidence that BPV is associated with the development of R-DWILs in primary ICH.</p
Interaction between Amyloid‑β Peptide and Heme Probed by Electrochemistry and Atomic Force Microscopy
Heme binds to amyloid β-peptide (Aβ) in the
brain of Alzheimer’s disease (AD) patients, thus forming Aβ-heme
complexes and leading the characteristic pathological features of
AD. The interaction between heme and Aβ might have important
biological relevance to AD etiology. In this work, the electrochemical
performances of heme after incubation with Aβ1–42, Aβ
fragments, and mutated Aβ were systematically investigated using
cyclic voltammetry and differential pulse voltammetry. Our results
indicated that His13 and His14 were possible binding sites, and Aβ
bound two molecules of heme with a binding constant of <i>K</i><sub>a1</sub> = 7.27 × 10<sup>6</sup> M<sup>–1</sup> (<i>n</i><sub>1</sub> = 1.5) and <i>K</i><sub>a2</sub> = 2.89 × 10<sup>6</sup> M<sup>–1</sup> (<i>n</i><sub>1</sub> = 1.8). Detailed analysis with atomic force microscopy
(AFM) of Aβ1–42 in the absence or presence of heme under
the same incubation conditions showed that heme inhibited the formation
of Aβ fibrils. According to results of the spectroscopic characterization,
Arg5 was the key residue in making the heme-Aβ1–42 complex
as a peroxidase
Changes in antioxidant enzyme activity in <i>E</i>. <i>crassipes</i> and <i>P</i>. <i>stratiotes</i> exposed to 100 mg/L Cd for different times.
<p>A: CAT activity. B: APX activity. C: GR activity. D: SOD activity. Data are presented as mean ± standard error. Different letters following mean values indicate significant differences (Tukey’s test, <i>P</i><0.05).</p
Comparative proteomics analyses of four <i>E</i>. <i>crassipes</i> samples treated with 100 mg/L Cd for different times.
<p>A: Representative 2-DE (the control sample of the first set of gel images) showing spot numbers of identified proteins. B: Hierarchical clustering of the identified protein expression profiles of different samples. Different colors correspond to the protein log-transformed fold-change ratios depicted in the bar on the left of the figure. C: Venn diagram analysis of differentially expressed proteins of each treated sample compared with the control sample (0d). “+” and “-” represent up-regulated and down-regulated proteins, respectively.</p
Changes in malondialdehyde (MDA) and proline content in <i>E</i>. <i>crassipes</i> and <i>P</i>. <i>stratiotes</i> exposed to 100 mg/L Cd for different times.
<p>A: MDA content change. B: Proline content change. Data are presented as mean ± standard error. Different letters following mean values indicate significant differences (Tukey’s test, <i>P</i><0.05).</p
Identification and analysis of differentially expressed proteins in leaves of <i>Eichhornia crassipes</i> treated by Cd stress for different times.
<p><sup>a</sup>Acc. No., database accession numbers according to NCBInr;</p><p><sup>b</sup>Theo. Mw/pI, theoretical Mw/pI;</p><p><sup>c</sup>Exp. Mw/pI, experimental Mw/pI;</p><p><sup>d</sup>SC, sequence coverage;</p><p><sup>e</sup>Ratio, different protein spot intensity ratios of samples after 2 d, 3 d and 5 d exposure relative to the control (0d).</p><p>Identification and analysis of differentially expressed proteins in leaves of <i>Eichhornia crassipes</i> treated by Cd stress for different times.</p
Functional classification of the identified proteins.
<p>Functional classification of the identified proteins.</p
Changes in photosynthetic characteristics and reactive oxygen species (ROS) of <i>E</i>. <i>crassipes</i> and <i>P</i>. <i>stratiotes</i> exposed to 100 mg/L Cd for different times.
<p>A: Photosynthetic rate change. B: Stomatal conductance change. C: <i>In situ</i> detection of H<sub>2</sub>O<sub>2</sub> and O<sub>2</sub><sup>-</sup>. Data are presented as mean ± standard error. Different letters following mean values indicate significant differences (Tukey’s test, <i>P</i><0.05).</p
Conductive Polymer-Coated VS<sub>4</sub> Submicrospheres As Advanced Electrode Materials in Lithium-Ion Batteries
VS<sub>4</sub> as an electrode material in lithium-ion batteries
holds intriguing features like high content of sulfur and one-dimensional
structure, inspiring the exploration in this field. Herein, VS<sub>4</sub> submicrospheres have been synthesized via a simple solvothermal
reaction. However, they quickly degrade upon cycling as an anode material
in lithium-ion batteries. So, three conductive polymers, polythiophene
(PEDOT), polypyrrole (PPY), and polyaniline (PANI), are coated on
the surface to improve the electron conductivity, suppress the diffusion
of polysulfides, and modify the interface between electrode/electrolyte.
PANI is the best in the polymers. It improves the Coulombic efficiency
to 86% for the first cycle and keeps the specific capacity at 755
mAh g<sup>–1</sup> after 50 cycles, higher than the cases of
naked VS<sub>4</sub> (100 mAh g<sup>–1</sup>), VS<sub>4</sub>@PEDOT (318 mAh g<sup>–1</sup>), and VS<sub>4</sub>@PPY (448
mAh g<sup>–1</sup>). The good performances could be attributed
to the improved charge-transfer kinetics and the strong interaction
between PANI and VS<sub>4</sub> supported by theoretical simulation.
The discharge voltage ∼2.0 V makes them promising cathode materials
Positive Synergy between the Helical Poly(phenylacetylene) Backbones and the Helical <i>L</i>‑Proline Oligopeptide Pendants for Enhanced Enantioseparation Properties
A series of optically active helical poly(phenylacetylene)s
(PPA-Pro1, PPA-Pro3, PPA-Pro6, PPA-Pro9, and PPA-Pro12) bearing different chain
lengths of L-proline oligopeptide in the side chains
were obtained
by polymerizing the corresponding novel phenylacetylene monomers.
The monomer adopted a trans-rich helix structure
when the L-proline oligopeptide chain length was
longer, according to the optical activities and 2D-NMR analysis. The
helical structure could be maintained and significantly influenced
the polymers’ helical conformation by introducing the L-proline oligopeptide to the pendants. By the way, the
morphology of PPA-Pro3 was observed
by atomic force microscope (AFM) on highly oriented pyrolytic graphite
(HOPG), and the information on the helix direction, pitch, and chain
arrangement was obtained. Also, the chiral separation properties of
these polymer-based chiral stationary phases (CSPs) were investigated
using high-performance liquid chromatography (HPLC). The poly(phenylacetylene)s
showed enhanced enantioseparation properties toward various racemates
depending on the longer chain length of the L-proline
oligopeptide in the pendants and the positive synergy between the
helical backbone and helical side chains. Particularly, PPA-Pro9 showed comparable or even superior enantioseparation
properties for racemates 2 and 9 to four commercial columns (Daicel
Chiralpak or Chiralcel AD, AS, OD, and OT), indicating that these
poly(phenylacetylene)-based CSPs have potential practical values.
This work presented here provides inspiration for the further development
of CSPs based on a new paradigm