Domains analysis of <i>At</i>GUS, <i>P</i>GUS and <i>Au</i>GUS.

a<p>the E value was obtained by alignment with pfam02837, glycosyl hydrolase family 2, sugar binding domain;</p>b<p>the E value was obtained by alignment with pfam00703, glycosyl hydrolase family 2, immunoglobulin-like beta-sandwich domain;</p>c<p>the E value was obtained by alignment with pfam02836, glycosyl hydrolase family 2, TIM barrel domain.</p

A three-dimensional model of β-glucuronidase(<i>At</i>GUS).

<p>I: sugar binding domain; II: immunoglobulin-like beta-sandwich domain; III: TIM barrel domain; IV: non-conservative domain; catalytic face and stability face were shown CF and SF for short.</p

Mental ion effect on <i>At</i>GUS-E and <i>At</i>GUS(-3t)-E.

<p>Mental ion effect on <i>At</i>GUS-E and <i>At</i>GUS(-3t)-E.</p

Far-UV spectra of <i>At</i>GUS-E and <i>At</i>GUS(-3t)-E.

<p>Far-UV spectra were recorded at 25°C in the range from 200 to 250 nm with a spectral resolution of 0.2 nm.</p

Intron deletion of <i>At</i>gus (A) and electrophoresis of the target sequence(B and C).

<p>M: marker, DL2000; L1: the target gene with intron; L2: the full length of the gene without intron(<i>At</i>gus); L3: the target gene without C-terminal non-conservative sequence[<i>At</i>gus(-3t)].</p

Enzymatic properties of <i>At</i>GUS-E and <i>At</i>GUS(-3t)-E.

<p>(A) the optimum pH, (B) the optimum temperature, and the thermal stability of <i>At</i>GUS(-3t)-E (C) and <i>At</i>GUS-E (D).</p

Additional file 1: of Effect of in ovo folic acid injection on hepatic IGF2 expression and embryo growth of broilers

The summary of regression analysis for methylation data. (DOC 42 kb

One-Step Hydrothermal Synthesis of High-Performance Gas-Sensing Crystals CdIn₂O₄ with Octahedral Shape

Octahedrally shaped CdIn₂O₄ was synthesized in a one-step hydrothermal reaction without any surfactant. These octahedral particles used as an ethanol sensor revealed excellent gas response, which is comparable to the well-known SnO₂-based gas sensors. The octahedral CdIn₂O₄ powder possesses the active {111} facets, which contribute to the higher gas-sensing performance of octahedral CdIn₂O₄ than that of the nanosized CdIn₂O₄ powder obtained by coprecipitation method. The XPS results demonstrated that the CdIn₂O₄ octahedra enclosed by {111} facets provided more oxygen vacancies, resulting in the enhanced performance. The calculation also showed higher surface energies for the {111} orientation, which confirms the mechanism of the enhanced gas-sensing performance of the octahedral particles. The as-formed crystals with controlled morphology provide insights for the designed strategy of high-performance gas-sensing materials, and this synthetic route provides flexibility and selectivity for the deliberate preparation of other functional materials

Genotype of rs11077 SNP site in XPO5 and its association with HCC survival.

<p>rs11077 SNP site in XPO5 (C/C: SNPs, A/C: heterozygous SNP, A/A:WT). Cum =  cumulative.</p

Phylogenetic tree of target protein with GHF2 β-glucuronidase (GUS), β-galactosidase (GAL), β-mannosidase (MAN).

<p>The scale corresponds to a genetic distance of 0.2 substitution per position (20% difference). <i>Aspergillus niger</i> CBS 513.88 GUS: XP_001388566; <i>Penicillium purpurogenum</i> GUS: ABU68712; <i>Aspergillus oryzae</i> RIB40 GUS: XP_001825002; <i>Penicillium canescens</i> GUS: AAV91787; <i>Escherichia coli</i> K-12 GUS: AAC74689; <i>Mus musculus</i> GUS: AAA37696; <i>Chlorocebus aethiops</i> GUS: AAC34593; <i>Canis lupus</i> GUS: AAC48809; <i>Thermotoga maritima</i> MSB8 GUS: AAD36143; <i>Kluyveromyces lactis</i> GAL: AAA35265; <i>Bacillus megaterium</i> DSM 319 GAL: CAA04267; <i>Lactobacillus sakei</i> GAL: CAA57730; <i>Pseudoalteromonas haloplanktis</i> GAL: CAA10470; <i>Escherichia coli</i> GAL: AAA24053; <i>Cellulomonas fimi</i> ATCC 484 MAN: AAD42775; <i>Aspergillus aculeatus</i> MAN: BAA29029; <i>Mus musculus</i> MAN: AAK18177; <i>Bos Taurus</i> MAN: AAC48460; <i>Homo sapiens</i> MAN: AAC39573.</p