Behavior and activity scores.

<p>Behavior and activity scores.</p

Ferritin immunoreactivity, and the ferritin heavy chain (FTH) and light chain (FTL) protein levels in cortex at day 3 after sham or subarachnoid hemorrhage induction with deferoxamine (DFX) treatment or vehicle, scale bar = 20μm.

<p>Values are mean ± SD; n = 3 for each group, #p<0.01 vs. SAH+vehicle group at day 3.</p

Effects of deferoxamine on blood-brain barrier disruption after subarachnoid hemorrhage - Fig 3

<p><b>(A</b>) Occludin immunoreactivity and protein levels in cortex after sham or subarachnoid hemorrhage induction with deferoxamine (DFX) treatment or vehicle at day 3, scale bar = 20μm. Values are mean ± SD; n = 3 for each group, #p<0.01, *p<0.05 vs. SAH+vehicle group at day 3. <b>(B)</b> ZO-1 immunoreactivity and protein levels in cortex after sham or subarachnoid hemorrhage induction with deferoxamine (DFX) treatment or vehicle at day 3, scale bar = 20μm. Values are mean ± SD; n = 3 for each group, #p<0.01 vs. SAH+vehicle group at day 3. <b>(C)</b> Claudin-5 immunoreactivity and protein levels in cortex after sham or subarachnoid hemorrhage induction with deferoxamine (DFX) treatment or vehicle at day 3, scale bar = 20μm. Values are mean ± SD; n = 3 for each group, *p<0.05 vs. SAH+vehicle group at day 3.</p

Univariate and multivariate analysis of variables associated with survival in PCa patients.

<p>HR = hazard ratio; CI = confidence interval; PSA = prostate-specific antigen.</p>a<p>not included in multivariate analysis.</p

Representative images for IHC staining and FISH analysis of <i>PTEN,</i> HER2 and Ki-67 expression in PCa.

<p>(A1–A3) FISH images of undeleted, hemizygous and homozygous <i>PTEN</i> deletion in PCa. A1, <i>PTEN</i> deletion negative case showed both paired red signals (10q23/<i>PTEN</i> locus) and green signals in tumor cells. A2, Representative case with <i>PTEN</i> hemizygous deletion showed one red signals and pairs of green signals in tumor cells. A3, Representative case with <i>PTEN</i> homozygous deletion showed absence of red signals but retained pairs of green signals. For all assays, at least 50 cancer cell nuclei were evaluated. (B1–B3) The detection of HER2 expression by IHC and FISH in PCa. B1, FISH analysis of representative case without <i>HER2</i> amplification. B2, FISH analysis of case with <i>HER2</i> amplification. B3, HER2 IHC staining shows complete membranous reactivity of strong intensity (3+) in tumor cells (original magnification, ×200). (C1–C3) The Ki-67 staining by IHC in PCa cells. C1, No staining (0) of Ki-67 in tumor cells. C2, Low Ki-67 (LI<10%) nuclear positivity in tumor cells. C3, High Ki-67(LI≥10%) nuclear positivity in tumor cells.</p

Kaplan-Meier survival analysis of PCa patients in relation to <i>ERG</i> rearrangement status.

<p>(A) low ki-67 LI (<10%) subgroup, (B) high ki-67 LI (≥10%) subgroup.</p

Clinicopathological demographics of 190 Chinese prostate cancer patients.

<p>Clinicopathological demographics of 190 Chinese prostate cancer patients.</p

FISH probe design and representative images of <i>ERG</i> rearrangement.

<p>(A) Schematic map of ‘<i>TMPRSS2</i>’ and ‘<i>ERG</i>’ position on 21q22.2–22.3. T and C orientate toward the telomeric and centromeric regions, respectively. BACs located 5′ and 3′ to <i>ERG</i> were used as probes for interphase FISH. Chromosomal coordinates are from the March 2006 build of the human genome using the UCSC Genome Browser. The <i>TMPRSS2</i>and <i>ERG</i> loci are separated by approximately 3 Mb. (B) FISH was performed using BACs as indicated with the corresponding fluorescent label on formalin-fixed paraffin-embedded tissue sections for break-apart FISH of the <i>ERG</i> gene. (B & E), <i>ERG</i> rearrangement negative case, as indicated by two pairs of co-localized green and red signals. (C & F), <i>ERG</i> rearrangement positive (translocation) case showed one pair of split 5′ and 3′ signals. (D & G), <i>ERG</i> rearrangement positive (with deletion) case showed loss of one green labeled probe 5′ to <i>ERG</i>.</p

Kaplan-Meier curves illustrating cancer related survival among PCa patients.

<p>The patients were stratified by <i>ERG</i> rearrangement and Ki-67 LI in combination and log-rank test was performed.</p

Kaplan-Meier survival curves for PCa patients with and without <i>ERG</i> rearrangement.

<p>The cancer-related survival rates were compared between patients with and without <i>ERG</i> rearrangement using the log-rank test.</p