Motif analysis of Kac peptides.

<p>(<b>A</b>) Acetylation motifs and conservation of acetylation sites. The height of each letter corresponds to the frequency with which that amino acid residue is found at that position. (<b>B</b>) Heat map representing the amino acid composition of the Kac sites, showing the frequency of the different types of amino acids surrounding Kac sites. (<b>C</b>) Cellular distribution of acetylated proteins and sites. (<b>D</b>) Sequence logo plots of normalized amino acid frequencies ±10 amino acids from the lysine acetylation site in cellular compartments. (<b>E</b>) Comparison analysis of acetylation motifs between <i>Aspergillus flavus</i>, <i>Phytophthora sojae</i>, <i>Botrytis cinerea</i>, and <i>Bacillus amyloliquefaciens</i>.</p

Protein information involved in synthesis of aflatoxin in <i>A</i>. <i>flavus</i>.

<p>Protein information involved in synthesis of aflatoxin in <i>A</i>. <i>flavus</i>.</p

Acetylation of metabolic enzymes identified as involved in glycolysis/gluconeogenesis and the citric acid cycle.

<p>The identified numbers of lysine-acetylated enzymes and proteins are shown in red. The identified Kac proteins found in mammalian cells are marked with●, those in <i>Escherichia coli</i> with ★, and those in <i>Saccharopolyspora erythraea</i> with▲.</p

Lysine acetylation status is analyzed by using SDS-PAGE and western blotting.

<p>(<b>A</b>) Confirmation of acetylated proteins present in <i>A</i>. <i>flavus</i>. 15μg protein samples were loaded for SDS-PAGE analysis. Acetylated Lys antibody (PTM Biolabs) was used in a 1:1000 dilution. (<b>B</b>) Distribution of lysine-acetylated peptides based on the number of acetylation sites.</p

Proteome-wide profiling of protein lysine acetylation in <i>Aspergillus flavus</i> - Fig 3

<p><b>(A)</b> GO, <b>(B)</b> subcellular-localization analysis, and <b>(C)</b> KEGG-pathway enrichment of the identified Kac proteins. (<b>D</b>) Comparison analysis of enriched pathways between <i>Aspergillus flavus</i>, <i>Phytophthora sojae</i>, <i>Botrytis cinerea</i>, and <i>Bacillus amyloliquefaciens</i>.</p

Comparisons of the protein and mRNA expression patterns of three representative DEPs at four artificial ageing stages (WH98, WH50, WH20, and WH01) by iTRAQ and qRT-PCR.

<p>Solid lines represent mRNA expression patterns, and dotted lines represent protein expression patterns.</p

Differentially expressed proteins and their functional classification analysis during artificial ageing.

<p>(A) Differentially expressed proteins during artificial ageing compared with unaged seeds; (B) sub-cellular localization analysis; (C) eukaryotic orthologous group (KOG) analysis.</p

Quantitative Proteomic Analysis of Wheat Seeds during Artificial Ageing and Priming Using the Isobaric Tandem Mass Tag Labeling

<div><p>Wheat (<i>Triticum aestivum</i> L.) is an important crop worldwide. The physiological deterioration of seeds during storage and seed priming is closely associated with germination, and thus contributes to plant growth and subsequent grain yields. In this study, wheat seeds during different stages of artificial ageing (45°C; 50% relative humidity; 98%, 50%, 20%, and 1% Germination rates) and priming (hydro-priming treatment) were subjected to proteomics analysis through a proteomic approach based on the isobaric tandem mass tag labeling. A total of 162 differentially expressed proteins (DEPs) mainly involved in metabolism, energy supply, and defense/stress responses, were identified during artificial ageing and thus validated previous physiological and biochemical studies. These DEPs indicated that the inability to protect against ageing leads to the incremental decomposition of the stored substance, impairment of metabolism and energy supply, and ultimately resulted in seed deterioration. Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis revealed that the up-regulated proteins involved in seed ageing were mainly enriched in ribosome, whereas the down-regulated proteins were mainly accumulated in energy supply (starch and sucrose metabolism) and stress defense (ascorbate and aldarate metabolism). Proteins, including hemoglobin 1, oleosin, agglutinin, and non-specific lipid-transfer proteins, were first identified in aged seeds and might be regarded as new markers of seed deterioration. Of the identified proteins, 531 DEPs were recognized during seed priming compared with unprimed seeds. In contrast to the up-regulated DEPs in seed ageing, several up-regulated DEPs in priming were involved in energy supply (tricarboxylic acid cycle, glycolysis, and fatty acid oxidation), anabolism (amino acids, and fatty acid synthesis), and cell growth/division. KEGG and protein-protein interaction analysis indicated that the up-regulated proteins in seed priming were mainly enriched in amino acid synthesis, stress defense (plant-pathogen interactions, and ascorbate and aldarate metabolism), and energy supply (oxidative phosphorylation and carbon metabolism). Therefore, DEPs associated with seed ageing and priming can be used to characterize seed vigor and optimize germination enhancement treatments. This work reveals new proteomic insights into protein changes that occur during seed deterioration and priming.</p></div

The protein-protein interaction network analysis of up-regulated proteins (A) and down-regulated proteins (B) identified by TMT-labeling during seed priming.

<p>The protein-protein interaction network analysis of up-regulated proteins (A) and down-regulated proteins (B) identified by TMT-labeling during seed priming.</p

Functional enrichment-based clustering of protein groups during artificial ageing.

<p>(A) Biological process; (B) Cellular component; (C) Molecular function; (D) KEGG pathway; (D) Protein domain.</p